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公开(公告)号：US20140020131A1
公开(公告)日：2014-01-16
申请号：US13526912
申请日：2012-06-19
申请人： Dennis L. Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：US20150184194A1
公开(公告)日：2015-07-02
申请号：US14129038
申请日：2012-06-19
申请人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：US09574208B2
公开(公告)日：2017-02-21
申请号：US14129038
申请日：2012-06-19
申请人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译： 公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。 所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。 还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。 包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物 进一步公开。
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公开(公告)号：US09234194B2
公开(公告)日：2016-01-12
申请号：US12706489
申请日：2010-02-16
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 还提供了包含FRT位点的文库群的试剂盒，以及制备修饰的FRT重组位点的文库的方法。 重组生成修饰的FRT重组位点可用于多种目标多重核苷酸重组方法。
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公开(公告)号：US06337100B1
公开(公告)日：2002-01-08
申请号：US09586959
申请日：2000-06-05
IPC分类号： A23D700
CPC分类号： C12N9/0083 , C12N15/8247
摘要： Antisense expression of a stearoyl-ACP desaturase gene in sunflower results in more than a four-fold increase in seed stearate, and can enhance palmitate content as well. Thus, sunflower oil containing over 40 percent saturated fatty acids can be produced, which is desirable in the context of various products, such as coating fat, margarine, soap and shortening.
摘要翻译： 向日葵中的硬脂酰-ACP去饱和酶基因的反义表达导致硬脂酸硬脂酸酯的增加超过4倍，并且还可以提高棕榈酸酯含量。 因此，可以生产含有超过40％的饱和脂肪酸的向日葵油，这在各种产品的情况下是可取的，例如包衣脂肪，人造奶油，肥皂和起酥油。
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6.发明申请Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开提高位点特异性多核苷酸交换效率的方法和组合公开(公告)号：US20070143881A1
公开(公告)日：2007-06-21
申请号：US11427947
申请日：2006-06-30
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
IPC分类号： A01H1/00
CPC分类号： C12N15/8213 , C12N15/8205 , C12N15/8207
摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.
摘要翻译： 使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。 所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率 ，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。 还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。
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公开(公告)号：US06709865B1
公开(公告)日：2004-03-23
申请号：US09714767
申请日：2000-11-16
申请人： Dennis L. Bidney , Jonathan P. Duvick , Carol Hendrick , Xu Hu , Guihua Lu , Oswald R. Crasta
发明人： Dennis L. Bidney , Jonathan P. Duvick , Carol Hendrick , Xu Hu , Guihua Lu , Oswald R. Crasta
IPC分类号： C12N500
CPC分类号： C12N9/0073 , C07K14/415 , C12N9/0006 , C12N15/8216 , C12N15/8237 , C12N15/8239 , C12N15/8261 , C12N15/8282 , Y02A40/146
摘要： Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a LOX protein are provided. Nucleotide sequences comprising the LOX promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译： 提供了调节发育和防御反应的方法和组合物。 提供了编码LOX蛋白的核苷酸序列。 还提供了包含LOX启动子的核苷酸序列。 序列可用于表达盒中用于调节发育，发育途径和植物防御反应。 还提供了转化的植物，植物细胞，组织和种子。
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公开(公告)号：US06380461B1
公开(公告)日：2002-04-30
申请号：US09525258
申请日：2000-03-14
申请人： Dennis L. Bidney , David G. Charne , Glenn S. Cole , Mark K. Mancl , Igor Falak , Katherine A. P. Nazarian , Christopher J. Scelonge
发明人： Dennis L. Bidney , David G. Charne , Glenn S. Cole , Mark K. Mancl , Igor Falak , Katherine A. P. Nazarian , Christopher J. Scelonge
IPC分类号： A01H500
CPC分类号： C12N9/0008 , C12N9/0006 , C12N9/88 , C12N15/8271 , C12N15/8282
摘要： Methods for increasing resistance in plants to pathogens by the expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme. The present invention relates to a method of producing a pathogen resistant hybrid plant by crossing the appropriate transgenic expressing a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme with pathogen tolerant lines or inbreds obtained through conventional genetic manipulations, or by transformation of tolerant plants or plant tissues with a hydrogen peroxide/reactive oxygen species producing gene or by altering the expression of an endogenase hydrogen peroxide/reactive oxygen species producing gene. The synergistic effect of expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme in a tolerant background gives significant and unexpectedly high resistance to the pathogens.
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公开(公告)号：US06265638B1
公开(公告)日：2001-07-24
申请号：US09407574
申请日：1999-09-28
IPC分类号： C12N1582
CPC分类号： C12N15/8241 , C12N15/8205 , C12N15/8209
摘要： Compositions and methods for the efficient co-transformation of a plant are provided. Novel compositions are Agrobacterium strains that have been engineered to comprise at least two binary vector plasmids in addition to a helper plasmid comprising the vir functions. Each of the binary vectors comprises its own T-DNA borders flanking a heterologous nucleotide sequence of interest. Methods of the invention comprise the use of these novel multiple-binary vector Agrobacterium strains to co-transform a plant. In this manner, heterologous nucleotide sequences of interest residing on different binary vectors can be independently introduced into the plant in a single transformation event and incorporated in the plant's nuclear DNA in an unlinked manner. The invention also provides for regenerated, fertile transgenic plants, transgenic seeds produced therefrom, and T1 and subsequent generations.
摘要翻译： 提供了植物有效共同转化的组合物和方法。 除了包含vir功能的辅助质粒之外，新型组合物是经过工程改造以包含至少两种二元载体质粒的农杆菌菌株。 每个二元载体包含在感兴趣的异源核苷酸序列侧翼的其自身的T-DNA边界。 本发明的方法包括使用这些新型多元二元载体农杆菌菌株共同转化植物。 以这种方式，驻留在不同二元载体上的感兴趣的异源核苷酸序列可以在单个转化事件中独立地引入植物中，并以非连接的方式并入植物的核DNA中。 本发明还提供了再生的，可育的转基因植物，由其产生的转基因种子，以及T1和随后的几代。
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公开(公告)号：US08318493B2
公开(公告)日：2012-11-27
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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