公开(公告)号：US06686172B2

公开(公告)日：2004-02-03

申请号：US10069847

申请日：2002-02-28

申请人： Naoto Matsuyama ,  Mina Fukuhara ,  Masaharu Takayama ,  Koji Mizuno

发明人： Naoto Matsuyama ,  Mina Fukuhara ,  Masaharu Takayama ,  Koji Mizuno

IPC分类号： C12Q134

CPC分类号： G01N33/6815 ,  C12Q1/26 ,  C12Q1/34 ,  Y10S435/968 ,  Y10S435/975

摘要： The present invention provides a method and a kit for detecting or quantitatively determining homocysteine rapidly and simply and with high sensitivity by oxidizing the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof in the presence of an SH reagent to produce hydrogen peroxide and determining the produced hydrogen peroxide by color development using an oxidative color-developing agent. By using the method and kit of the present invention, homocysteine in biological samples, in particular, in body fluids such as blood and urine can be detected and quantitatively determined rapidly and simply and with high sensitivity.

摘要翻译： 本发明提供一种方法和试剂盒，用于通过在SH试剂存在下氧化残留的同型半胱氨酸共底物，产生的同型半胱氨酸转化酶产物或其酶反应产物，快速且简单地并且具有高灵敏度来检测或定量测定同型半胱氨酸， 产生过氧化氢，并使用氧化显色剂通过显色测定产生的过氧化氢。 通过使用本发明的方法和试剂盒，生物样品中的同型半胱氨酸，特别是体液如血液和尿液中的高半胱氨酸可以快速且简单且高灵敏度地进行检测和定量测定。