公开(公告)号：US06686172B2

公开(公告)日：2004-02-03

申请号：US10069847

申请日：2002-02-28

申请人： Naoto Matsuyama ,  Mina Fukuhara ,  Masaharu Takayama ,  Koji Mizuno

发明人： Naoto Matsuyama ,  Mina Fukuhara ,  Masaharu Takayama ,  Koji Mizuno

IPC分类号： C12Q134

CPC分类号： G01N33/6815 ,  C12Q1/26 ,  C12Q1/34 ,  Y10S435/968 ,  Y10S435/975

摘要： The present invention provides a method and a kit for detecting or quantitatively determining homocysteine rapidly and simply and with high sensitivity by oxidizing the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof in the presence of an SH reagent to produce hydrogen peroxide and determining the produced hydrogen peroxide by color development using an oxidative color-developing agent. By using the method and kit of the present invention, homocysteine in biological samples, in particular, in body fluids such as blood and urine can be detected and quantitatively determined rapidly and simply and with high sensitivity.

摘要翻译： 本发明提供一种方法和试剂盒，用于通过在SH试剂存在下氧化残留的同型半胱氨酸共底物，产生的同型半胱氨酸转化酶产物或其酶反应产物，快速且简单地并且具有高灵敏度来检测或定量测定同型半胱氨酸， 产生过氧化氢，并使用氧化显色剂通过显色测定产生的过氧化氢。 通过使用本发明的方法和试剂盒，生物样品中的同型半胱氨酸，特别是体液如血液和尿液中的高半胱氨酸可以快速且简单且高灵敏度地进行检测和定量测定。

公开(公告)号：US5801178A

公开(公告)日：1998-09-01

申请号：US597983

申请日：1996-02-07

申请人： Kazunaga Yazawa ,  Kazuo Watanabe ,  Yasuharu Ijuin ,  Mayumi Shikano ,  Yasuji Soda ,  Tetsuya Kosaka ,  Naoto Matsuyama ,  Koji Mizuno

发明人： Kazunaga Yazawa ,  Kazuo Watanabe ,  Yasuharu Ijuin ,  Mayumi Shikano ,  Yasuji Soda ,  Tetsuya Kosaka ,  Naoto Matsuyama ,  Koji Mizuno

IPC分类号： C07C233/09 ,  C07C233/15 ,  C07C233/27 ,  C07C233/38 ,  C07C275/28 ,  C07C275/40 ,  C07D295/18 ,  A61K31/495 ,  A61K31/165 ,  C07D295/185

CPC分类号： C07C233/38 ,  C07C233/09 ,  C07C233/15 ,  C07C233/27 ,  C07C275/28 ,  C07C275/40 ,  C07D295/18

摘要： An aniline derivative of the formula (1) ##STR1## wherein R.sup.1 is an eicosapentaenoyl or docosahexaenoyl; R.sup.2 and R.sup.3 are each independently an alkyl or alkoxy having 1 to 6 carbon atoms, or a halogen atom; R.sup.4 is a hydrogen atom, an alkyl or alkoxy having 1 to 6 carbon atoms, or a halogen atom; and A is a single bond, --C(.dbd.O)NH--(CH.sub.2).sub.n --NH-- wherein n is 2 or 3, or a bivalent group of the following formula ##STR2## wherein m and p are each independently 0 or 1. The aniline derivative of the present invention has high inhibitory activity against and high selectivity for ACAT derived from macrophage and is useful as an agent for the prophylaxis and treatment of arteriosclerosis.

摘要翻译： 式（1）的苯胺衍生物其中R 1是二十碳五烯酰基或二十二碳六烯酰基; R2和R3各自独立地为具有1至6个碳原子的烷基或烷氧基或卤素原子; R4是氢原子，具有1至6个碳原子的烷基或烷氧基或卤素原子; 并且A是单键，-C（= O）NH-（CH 2）n -NH-，其中n是2或3，或下式的二价基团，其中m和p各自独立地 0或1.本发明的苯胺衍生物对来自巨噬细胞的ACAT具有高的抑制活性和高选择性，可用作预防和治疗动脉硬化的药剂。

公开(公告)号：US07135306B2

公开(公告)日：2006-11-14

申请号：US10546089

申请日：2004-01-28

申请人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

发明人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

IPC分类号： C12Q1/34 ,  C12Q1/37 ,  G01N33/53

CPC分类号： C12Q1/26 ,  C12N9/1007 ,  C12Q2326/00 ,  G01N33/6815

摘要： A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.

摘要翻译： 用于检测或测量样品中同型半胱氨酸的方法包括以下步骤：（a）将样品中存在的D-氨基酸与D-氨基酸转化酶反应，以将D-氨基酸转化为不作为 D-氨基酸氧化酶或D-氨基酸乙酰转移酶的底物; （b）用硫醇化合物还原样品中的高半胱氨酸; （c）使还原的高半胱氨酸与甲基转移酶和甲基供体反应以新产生D-氨基酸; 和（d）在SH试剂的存在下使所产生的D-氨基酸与D-氨基酸氧化酶或D-氨基酸乙酰转移酶反应以产生过氧化氢，并且通过使用氧化颜色显色所产生的过氧化氢 发展代理

公开(公告)号：US20060166300A1

公开(公告)日：2006-07-27

申请号：US10546089

申请日：2004-01-28

申请人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

发明人： Nobuyoshi Esaki ,  Tohru Yoshimura ,  Naoto Matsuyama ,  Koji Mizuno ,  Takayuki Bogaki ,  Toshitaka Minetoki ,  Kenji Ozeki

IPC分类号： C12Q1/48

CPC分类号： C12Q1/26 ,  C12N9/1007 ,  C12Q2326/00 ,  G01N33/6815

摘要： A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino acid acetyltransferase; (b) reducing homocysteine in the sample with a thiol compound; (c) reacting the reduced homocysteine with a methyltransferase and a methyl donor to newly produce D-amino acid; and (d) reacting the produced D-amino acid with the D-amino acid oxidase or the D-amino acid acetyltransferase in the presence of an SH reagent to produce hydrogen peroxide, and color-developing the produced hydrogen peroxide by using an oxidative color-developing agent.