公开(公告)号：US06770453B1

公开(公告)日：2004-08-03

申请号：US09519533

申请日：2000-03-06

申请人： Steven J. Potts ,  David C. Slaughter ,  James F. Thompson ,  Jennifer J. Payne

发明人： Steven J. Potts ,  David C. Slaughter ,  James F. Thompson ,  Jennifer J. Payne

IPC分类号： C12Q134

CPC分类号： C12Q1/34 ,  G01N33/5308 ,  G01N33/56961 ,  G01N2333/42 ,  G01N2333/924 ,  G01N2400/28 ,  G01N2400/46

摘要： Methods for the detection of chitinous contaminants of non-chitinous biological materials are described. The methods are accurate, highly reproducible, rapid and relatively inexpensive. The methods are well suited to commercial applications, particular in the food and agriculture industry where biological materials (e.g., food products) are regularly screened for contaminants (e.g., insect, mold, fungus, etc.). In some cases, the methods involve contacting a biological sample with a probe that is a lectin that binds chitin, contacting the sample with a pectinase; and detecting binding of said lectin to a chitin where the binding indicates the presence of chitin in the biological sample.

摘要翻译： 描述了检测非壳质生物材料的几丁质污染物的方法。 这些方法是准确的，高度可重复的，快速的和相对便宜的。 这些方法非常适合商业应用，特别是在食品和农业工业中，其中定期筛选污染物（例如昆虫，霉菌，真菌等）的生物材料（例如食品）。 在一些情况下，所述方法包括使生物样品与作为凝集素结合几丁质的探针接触，使样品与果胶酶接触; 并检测所述凝集素与几丁质的结合，其中所述结合指示生物样品中几丁质的存在。

公开(公告)号：US06750034B1

公开(公告)日：2004-06-15

申请号：US09607745

申请日：2000-06-30

申请人： Andrew L. Darrow ,  Jenson (Jian-Shen) Qi ,  Patricia Andrade-Gordon

发明人： Andrew L. Darrow ,  Jenson (Jian-Shen) Qi ,  Patricia Andrade-Gordon

IPC分类号： C12Q134

CPC分类号： C12N9/6454 ,  A61K38/00 ,  Y10T436/24

摘要： Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed D-G. The deduced amino acid sequence, and it's alignment with other well characterized serine proteases clearly indicates that it is a member of the S1 serine protease family. We have found that the protease D-G mRNA is widely expressed in several tissues throughout the body including epidermis, fibroblasts, keratinocytes, colon, small intestine, stomach, lung, kidney, bone marrow, lymph node, thymus, ovary, prostate, uterus and spinal cord. Interestingly, this protease contains a hydrophobic stretch of amino acids which is a putative transmembrane near the NH2-terminus. Thus, this serine protease is thought to be synthesized as a type II integral protein. We expressed a soluble form of this novel human protease by inserting the portion of the protease D-G cDNA, encoding the catalytic domain, in a zymogen activation construct designed to permit the generic activation of heterologous serine protease catalytic domains. The result is an active preparation of protease D-G that has an activity against a subset of amidolytic substrates. This enzymatically active protease D-G preparation is now amenable to further biochemical analyses for the identification of physiological substrates as well as specific inhibitors.

摘要翻译： 这里我们描述了编码我们称为D-G的新型丝氨酸蛋白酶的cDNA的分子鉴定。 推导的氨基酸序列，与其他良好表征的丝氨酸蛋白酶的比对清楚地表明它是S1丝氨酸蛋白酶家族的成员。 我们已经发现蛋白酶DG mRNA在全身的几种组织中广泛表达，包括表皮，成纤维细胞，角质形成细胞，结肠，小肠，胃，肺，肾，骨髓，淋巴结，胸腺，卵巢，前列腺，子宫和脊髓 绳子 有趣的是，该蛋白酶含有氨基酸的疏水延伸，氨基酸是NH2末端附近的推定跨膜。 因此，认为该丝氨酸蛋白酶被合成为II型整合蛋白。 我们通过将编码催化结构域的蛋白酶D-G cDNA的部分插入设计用于允许异源丝氨酸蛋白酶催化结构域的通用活化的酶原激活构建体中来表达这种新型人类蛋白酶的可溶形式。 其结果是蛋白酶D-G的活性制备，其具有对酰氨基底物的子集的活性。 这种酶活性蛋白酶D-G制剂现在适合进一步的生物化学分析用于鉴定生理底物以及特异性抑制剂。

公开(公告)号：US06667161B1

公开(公告)日：2003-12-23

申请号：US09412896

申请日：1999-10-05

申请人： Stephen C. Johnson ,  Ming Lou ,  Shijia Yan

发明人： Stephen C. Johnson ,  Ming Lou ,  Shijia Yan

IPC分类号： C12Q134

CPC分类号： C07H15/203 ,  C07H7/027 ,  C07H15/24 ,  C07H15/26 ,  C07H17/02 ,  C07H17/075 ,  C12Q1/34

摘要： The current invention relates to the design, synthesis, and biochemical evaluation of chromogenic substrate compounds for sialidases of bacterial, viral, protozoa, and vertebrate (including human) origin. In particular, this invention provides a novel class of effective compounds as chromogenic substrates of these sialidases which yield chromogenic products after reactions catalyzed by sialidase take place. Also provided are methods of making these substrate compounds, methods of diagnosis and prognosis of sialidase related diseases using these substrate compounds.

摘要翻译： 本发明涉及用于细菌，病毒，原生动物和脊椎动物（包括人）来源的唾液酸酶的显色底物化合物的设计，合成和生物化学评估。 特别地，本发明提供了一种新型的有效化合物作为这些唾液酸酶的显色底物，其在由唾液酸酶催化的反应发生后产生显色产物。 还提供了使用这些底物化合物制备这些底物化合物的方法，唾液酸酶相关疾病的诊断和预后的方法。

公开(公告)号：US06440683B1

公开(公告)日：2002-08-27

申请号：US09246290

申请日：1999-02-08

申请人： Chuanzheng Song ,  Ronald Kriz ,  John Knopf

发明人： Chuanzheng Song ,  Ronald Kriz ,  John Knopf

IPC分类号： C12Q134

CPC分类号： C12N9/18 ,  C12N9/20

摘要： The invention provides a novel calcium-independent cytosolic phospholipase A2-Gamma enzyme, polynucleotides encoding such enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade.

摘要翻译： 本发明提供了一种新的不依赖钙的胞质磷脂酶A2-Gamma酶，编码这种酶的多核苷酸以及用于筛选由花生四烯酸级联介导的抗炎活性的未知化合物的方法。

公开(公告)号：US06387643B1

公开(公告)日：2002-05-14

申请号：US09252586

申请日：1999-02-18

申请人： Robert Leroy Heinrikson ,  Michael B. Fairbanks ,  Ana M. Mildner

发明人： Robert Leroy Heinrikson ,  Michael B. Fairbanks ,  Ana M. Mildner

IPC分类号： C12Q134

CPC分类号： C12Y302/01031 ,  C07K16/40 ,  C12N9/2402 ,  C12Y302/01166

摘要： The present invention provides isolated human heparanase polypeptides, and the isolated polynucleotide molecules that encode them, as well as vectors and host cells comprising such polynucleotide molecules. The invention also provides a method for the identification of an agent that alters heparanase activity.

摘要翻译： 本发明提供了分离的人肝素酶多肽，以及编码它们的分离的多核苷酸分子，以及包含这种多核苷酸分子的载体和宿主细胞。 本发明还提供了鉴定改变乙酰肝素酶活性的药剂的方法。

公开(公告)号：US06379911B2

公开(公告)日：2002-04-30

申请号：US09125808

申请日：1999-02-22

申请人： Vern L. Schramm ,  Richard Hubert Furneaux ,  Peter Charles Tyler ,  Keith Clinch

发明人： Vern L. Schramm ,  Richard Hubert Furneaux ,  Peter Charles Tyler ,  Keith Clinch

IPC分类号： C12Q134

CPC分类号： C07H21/00 ,  C12Q1/34 ,  Y10S435/975

摘要： The invention relates to a method of detecting and/or assaying nucleoside hydrolases or nucleoside phosphorylases using a chromogenic substrate. Preferred chromogenic substrates have formula (I) where X is OH, or H, and Y is the residue of Y—OH where Y—OH is a chromophore or a compound readily converted to a chromophore and the substrates are hydrolyzed by the nucleoside hydrolase to yield ribose or 2-deoxyribose plus Y—OH. Alternatively, those substrates may be phosphorylysed by nucleoside phosphorylase to yield ribose-1-phosphate plus Y—OH. The methods may be used to detect and/or assay parasites in biological samples.

摘要翻译： 本发明涉及使用显色底物检测和/或测定核苷水解酶或核苷磷酸酶的方法。 优选的显色底物具有式（I），其中X是OH或H，Y是Y-OH的残基，其中Y-OH是发色团或易于转化为发色团的化合物，并且底物被核苷水解酶水解成 产生核糖或2-脱氧核糖加Y-OH。 或者，这些底物可以被核苷磷酸化酶磷酸化以产生核糖-1-磷酸加上Y-OH。 该方法可用于检测和/或测定生物样品中的寄生虫。

公开(公告)号：US06340573B1

公开(公告)日：2002-01-22

申请号：US09646967

申请日：2000-11-15

申请人： Lyle Armstrong ,  Arthur James ,  Daniel Monget ,  Sylvain Orenga

发明人： Lyle Armstrong ,  Arthur James ,  Daniel Monget ,  Sylvain Orenga

IPC分类号： C12Q134

CPC分类号： C12Q1/04 ,  C12Q1/34 ,  C12Q2334/00 ,  C12Q2334/30 ,  G01N2333/924 ,  G01N2333/948 ,  Y10S435/975

摘要： The invention concerns a chromogenous substrate for detecting the presence of at least one enzyme enzymatic activity. The invention also concerns a method for using such a substrate. The invention further concerns a method for identification based on such substrates and a device for implementing said identification method. The invention is characterized in that the substrate consists of at lease two molecules, a first molecule consisting of a non-chromogenous marker part associated with at least a specific target part for the enzyme and a second molecule consisting of a non-chromogenous part, and the non-chromogenous marker part, once it is released, reacts with the second molecule to form a chromogenous molecule. The invention is particularly applicable in bacteriology.

摘要翻译： 本发明涉及用于检测至少一种酶酶活性的存在的发色底物。 本发明还涉及一种使用这种衬底的方法。 本发明还涉及基于这种基板的识别方法和用于实现所述识别方法的装置。 本发明的特征在于，底物由至少两个分子组成，第一分子由与酶的至少一个特定目标部分相关的非发色标记部分和由非发色部分组成的第二分子组成，以及 非发色标记部分一旦释放，就与第二个分子反应形成一个发色分子。 本发明特别适用于细菌学。

公开(公告)号：US06197537B1

公开(公告)日：2001-03-06

申请号：US09314362

申请日：1999-05-19

申请人： Srinivasa K. Rao ,  Leslie Kushner ,  Mahesh Mathrubutham ,  Jon R. Cohen

发明人： Srinivasa K. Rao ,  Leslie Kushner ,  Mahesh Mathrubutham ,  Jon R. Cohen

IPC分类号： C12Q134

CPC分类号： C12Q1/34 ,  C12Q1/37

摘要： The present invention provides an assay for detecting and quantifying protease that is sensitive, rapid, and capable of screening numerous samples of small volume. Also provided by the present invention is a method for diagnosing disorders associated with connective tissue destruction in a subject by detecting and quantifying a particular protease in a biological sample. Further provided by the present invention is a method of detecting inhibitors of the activity of a protease by detecting primary amines that are exposed by the enzymatic degradation of a protease substrate.

摘要翻译： 本发明提供了一种用于检测和定量蛋白酶的测定法，该蛋白酶敏感，快速并且能够筛选出许多小体积的样品。 本发明还提供了通过检测和定量生物样品中的特定蛋白酶来诊断与受试者中结缔组织破坏相关的疾病的方法。 本发明进一步提供的是通过检测通过蛋白酶底物的酶降解暴露的伯胺来检测蛋白酶活性的抑制剂的方法。

公开(公告)号：US06174695B1

公开(公告)日：2001-01-16

申请号：US09312207

申请日：1999-05-14

申请人： Bruce D. Hammock ,  Mehran F. Moghaddam ,  Jeffrey M. Cheek ,  Babak Borhan ,  James Fergusson ,  David F. Grant ,  Jessica F. Greene ,  Kazuhiko Matoba ,  Jiang Zheng ,  Marlene F. Sisemore

发明人： Bruce D. Hammock ,  Mehran F. Moghaddam ,  Jeffrey M. Cheek ,  Babak Borhan ,  James Fergusson ,  David F. Grant ,  Jessica F. Greene ,  Kazuhiko Matoba ,  Jiang Zheng ,  Marlene F. Sisemore

IPC分类号： C12Q134

CPC分类号： C12Q1/34 ,  G01N2500/10

摘要： The present invention provides methods of treating inflammatory diseases mediated by poly-unsaturated lipid metabolites by inhibiting epoxide hydrolase, methods for assaying or screening the epoxide hydrolase inhibitors for inhibitory specificity and for toxicity, and novel biologically active tetrahydrofuran diols of arachidonic acid, including antibodies thereto.

摘要翻译： 本发明提供了通过抑制环氧化物水解酶来治疗由多不饱和脂质代谢物介导的炎性疾病的方法，用于测定或筛选环氧化物水解酶抑制剂以抑制特异性和毒性的方法，以及花生四烯酸的新型生物活性四氢呋喃二醇，包括其抗体 。

公开(公告)号：US06783948B1

公开(公告)日：2004-08-31

申请号：US09626566

申请日：2000-07-27

申请人： Qingping Jiang ,  Anand Natrajan ,  David J. Sharpe ,  Wen-Jee Wong ,  Say-Jong Law

发明人： Qingping Jiang ,  Anand Natrajan ,  David J. Sharpe ,  Wen-Jee Wong ,  Say-Jong Law

IPC分类号： C12Q134

CPC分类号： C07D491/10 ,  C07D219/04 ,  C07F9/64 ,  C12Q1/42 ,  C12Q2334/00 ,  G01N33/533 ,  G01N33/582

摘要： A chemiluminescent substrate of hydrolytic enzyme having the following general Formula I, as follows: Lumi-M-P  Formula I where “Lumi” is a chemiluminescent moiety capable of producing light (a) by itself, (b) with MP attached and (c) with M attached. Examples of Lumi include chemiluminescent acridinium compounds, benzacridinium compounds, quinolinium compounds, isoquinolinium compounds, phenanthridinium compounds, and lucigenin compounds, spiroacridan compounds, luminol compounds and isoluminol compounds. M is a multivalent heteroatom having at least one lone pair of electrons selected from oxygen, nitrogen and sulfur, directly attached to the light emitting moiety of Lumi at one end and to P at the other end. P it a group that can be readily removed by hydrolytic enzymes. An enzymatic reaction utilizing the above compound is the following: where HE is a hydrolytic enzyme Lumi-M is a chemiluminescent product having physical and/or chemical properties different from those of Lumi-M-P.

摘要翻译： 具有以下通式I的水解酶的化学发光底物如下：其中“Lumi”是能够自身产生光的化学发光部分（a），（b）MP附着的（c）和M连接的化合物发光部分。 Lumi的实例包括化学发光吖啶鎓化合物，苯并吖啶鎓化合物，喹啉鎓化合物，异喹啉鎓化合物，菲啶鎓化合物和光合精化合物，螺吡啶化合物，鲁米诺化合物和异鲁米诺化合物。 M是具有至少一个选自氧，氮和硫的孤对电子的多价杂原子，其一端直接连接到Lumi的发光部分，另一端直接附着于P。 P是可以通过水解酶容易地除去的基团。 使用上述化合物的酶反应如下：其中HE是水解酶，Lumi-M是具有与Lumi-M-P不同的物理和/或化学性质的化学发光产物。