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公开(公告)号:US08598329B2
公开(公告)日:2013-12-03
申请号:US12941557
申请日:2010-11-08
IPC分类号: C07H21/04
CPC分类号: C12N15/8243 , C07K14/8139 , C12N9/50 , C12N15/8251
摘要: The present invention relates to isolated polynucleotides encoding cysteine proteinases; cysteine proteinase inhibitors; and aspartic endoproteinases. The invention also relates to a transformed host cell, preferably a plant cell, in which over- or under-expression of these polynucleotides result in altered levels of coffee flavor precursor levels, specifically, amino group-containing molecules such as amino acids, peptides and proteins, in green coffee grains.
摘要翻译: 本发明涉及编码半胱氨酸蛋白酶的分离的多核苷酸; 半胱氨酸蛋白酶抑制剂; 和天冬氨酸内蛋白酶。 本发明还涉及转化的宿主细胞,优选植物细胞,其中这些多核苷酸的过表达或低表达导致咖啡风味前体水平的改变,特别是含氨基的分子,例如氨基酸,肽和 蛋白质,在绿色咖啡谷物。
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公开(公告)号:US08513491B2
公开(公告)日:2013-08-20
申请号:US11922978
申请日:2006-07-05
CPC分类号: C12N15/8234 , C07K14/415 , C12N15/8273
摘要: Dehydrin- and late embryogenic abundant (LEA) protein-encoding polynucleotides from coffee plants are disclosed. Also disclosed are a promoter sequence from a coffee dehydrin gene, and methods for using these polynucleotides and promoter sequences for gene regulation and manipulation of flavor, aroma, stress tolerance and other features of coffee beans.
摘要翻译: 公开了来自咖啡植物的脱水和晚期胚发生丰富(LEA)蛋白质编码多核苷酸。 还公开了来自咖啡脱水蛋白基因的启动子序列,以及使用这些多核苷酸和启动子序列进行基因调节和操作风味,香气,胁迫耐受性和咖啡豆的其它特征的方法。
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公开(公告)号:US20100154073A1
公开(公告)日:2010-06-17
申请号:US11922978
申请日:2006-07-05
CPC分类号: C12N15/8234 , C07K14/415 , C12N15/8273
摘要: Dehydrin- and late embryogenic abundant (LEA) protein-encoding polynucleotides from coffee plants are disclosed. Also disclosed are a promoter sequence from a coffee dehydrin gene, and methods for using these polynucleotides and promoter sequences for gene regulation and manipulation of flavor, aroma, stress tolerance and other features of coffee beans.
摘要翻译: 公开了来自咖啡植物的脱水和晚期胚发生丰富(LEA)蛋白质编码多核苷酸。 还公开了来自咖啡脱水蛋白基因的启动子序列,以及使用这些多核苷酸和启动子序列进行基因调节和操作风味,香气,胁迫耐受性和咖啡豆的其它特征的方法。
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4.
公开(公告)号:US20110126314A1
公开(公告)日:2011-05-26
申请号:US12941557
申请日:2010-11-08
CPC分类号: C12N15/8243 , C07K14/8139 , C12N9/50 , C12N15/8251
摘要: The present invention relates to isolated polynucleotides encoding cysteine proteinases; cysteine proteinase inhibitors; and aspartic endoproteinases. The invention also relates to a transformed host cell, preferably a plant cell, in which over- or under-expression of these polynucleotides result in altered levels of coffee flavour precursor levels, specifically, amino group-containing molecules such as amino acids, peptides and proteins, in green coffee grains.
摘要翻译: 本发明涉及编码半胱氨酸蛋白酶的分离的多核苷酸; 半胱氨酸蛋白酶抑制剂; 和天冬氨酸内蛋白酶。 本发明还涉及转化的宿主细胞,优选植物细胞,其中这些多核苷酸的过表达或低表达导致咖啡风味前体水平的改变,特别是含氨基的分子,例如氨基酸,肽和 蛋白质,在绿色咖啡谷物。
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