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公开(公告)号：US08513491B2
公开(公告)日：2013-08-20
申请号：US11922978
申请日：2006-07-05
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8273
摘要： Dehydrin- and late embryogenic abundant (LEA) protein-encoding polynucleotides from coffee plants are disclosed. Also disclosed are a promoter sequence from a coffee dehydrin gene, and methods for using these polynucleotides and promoter sequences for gene regulation and manipulation of flavor, aroma, stress tolerance and other features of coffee beans.
摘要翻译： 公开了来自咖啡植物的脱水和晚期胚发生丰富（LEA）蛋白质编码多核苷酸。 还公开了来自咖啡脱水蛋白基因的启动子序列，以及使用这些多核苷酸和启动子序列进行基因调节和操作风味，香气，胁迫耐受性和咖啡豆的其它特征的方法。
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公开(公告)号：US20100154073A1
公开(公告)日：2010-06-17
申请号：US11922978
申请日：2006-07-05
CPC分类号： C12N15/8234 , C07K14/415 , C12N15/8273
摘要： Dehydrin- and late embryogenic abundant (LEA) protein-encoding polynucleotides from coffee plants are disclosed. Also disclosed are a promoter sequence from a coffee dehydrin gene, and methods for using these polynucleotides and promoter sequences for gene regulation and manipulation of flavor, aroma, stress tolerance and other features of coffee beans.
摘要翻译： 公开了来自咖啡植物的脱水和晚期胚发生丰富（LEA）蛋白质编码多核苷酸。 还公开了来自咖啡脱水蛋白基因的启动子序列，以及使用这些多核苷酸和启动子序列进行基因调节和操作风味，香气，胁迫耐受性和咖啡豆的其它特征的方法。
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3.发明授权Nucleic acids and proteins associated with galactomannan synthesis in coffee 有权与咖啡中半乳甘露聚糖合成相关的核酸和蛋白质公开(公告)号：US08217226B2
公开(公告)日：2012-07-10
申请号：US12083467
申请日：2006-10-16
CPC分类号： C12N9/1051 , C12N9/1048 , C12N15/8246
摘要： Disclosed herein are nucleic acid molecules isolated from coffee (Coffea spp.) comprising sequences that encode mannan synthase or galactomannan galactosyltransferase. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the polysaccharide molecules of coffee plants, to influence extraction characteristics and other features of coffee beans.
摘要翻译： 本文公开了从包含编码甘露聚糖合酶或半乳甘露聚糖半乳糖基转移酶的序列的咖啡（咖啡属物种）中分离的核酸分子。 还公开了使用这些多核苷酸进行咖啡植物的多糖分子的基因调节和操作以影响咖啡豆的提取特性和其它特征的方法。
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4.发明授权公开(公告)号：US08269065B2
公开(公告)日：2012-09-18
申请号：US11992612
申请日：2006-09-27
申请人： Isabelle Muguette Privat , James Gèrard McCarthy , Vincent Petiard , Steven D. Tanksley , Chenwei Lin
发明人： Isabelle Muguette Privat , James Gèrard McCarthy , Vincent Petiard , Steven D. Tanksley , Chenwei Lin
CPC分类号： C12N9/2408 , C12N15/8243 , C12N15/8245 , C12N15/8261 , C12Y302/01026 , Y02A40/146
摘要： Disclosed herein are nucleic acid molecules isolated from coffee (Coffea spp.) comprising sequences that encodes various sucrose metabolizing enzymes, along with their encoded proteins. Specifically, three types of invertase and four invertase inhibitors and their encoding polynucleotides from coffee are disclosed. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the sugar profile of coffee plants, to influence flavor, aroma, and other features of coffee beans.
摘要翻译： 本文公开了从包含编码各种蔗糖代谢酶的序列及其编码的蛋白质的咖啡（咖啡属）分离的核酸分子。 具体地，公开了三种类型的转化酶和四种转化酶抑制剂及其来自咖啡的编码多核苷酸。 还公开了使用这些多核苷酸用于咖啡植物的糖型的基因调节和操作以影响咖啡豆的风味，香味和其它特征的方法。
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公开(公告)号：US08598329B2
公开(公告)日：2013-12-03
申请号：US12941557
申请日：2010-11-08
IPC分类号： C07H21/04
CPC分类号： C12N15/8243 , C07K14/8139 , C12N9/50 , C12N15/8251
摘要： The present invention relates to isolated polynucleotides encoding cysteine proteinases; cysteine proteinase inhibitors; and aspartic endoproteinases. The invention also relates to a transformed host cell, preferably a plant cell, in which over- or under-expression of these polynucleotides result in altered levels of coffee flavor precursor levels, specifically, amino group-containing molecules such as amino acids, peptides and proteins, in green coffee grains.
摘要翻译： 本发明涉及编码半胱氨酸蛋白酶的分离的多核苷酸; 半胱氨酸蛋白酶抑制剂; 和天冬氨酸内蛋白酶。 本发明还涉及转化的宿主细胞，优选植物细胞，其中这些多核苷酸的过表达或低表达导致咖啡风味前体水平的改变，特别是含氨基的分子，例如氨基酸，肽和 蛋白质，在绿色咖啡谷物。
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公开(公告)号：US20110126314A1
公开(公告)日：2011-05-26
申请号：US12941557
申请日：2010-11-08
CPC分类号： C12N15/8243 , C07K14/8139 , C12N9/50 , C12N15/8251
摘要： The present invention relates to isolated polynucleotides encoding cysteine proteinases; cysteine proteinase inhibitors; and aspartic endoproteinases. The invention also relates to a transformed host cell, preferably a plant cell, in which over- or under-expression of these polynucleotides result in altered levels of coffee flavour precursor levels, specifically, amino group-containing molecules such as amino acids, peptides and proteins, in green coffee grains.
摘要翻译： 本发明涉及编码半胱氨酸蛋白酶的分离的多核苷酸; 半胱氨酸蛋白酶抑制剂; 和天冬氨酸内蛋白酶。 本发明还涉及转化的宿主细胞，优选植物细胞，其中这些多核苷酸的过表达或低表达导致咖啡风味前体水平的改变，特别是含氨基的分子，例如氨基酸，肽和 蛋白质，在绿色咖啡谷物。
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7.发明申请公开(公告)号：US20090313728A1
公开(公告)日：2009-12-17
申请号：US12083432
申请日：2006-10-16
申请人： Maud Lepelley , James Gérard McCarthy , Vincent Petiard , Gerald Cheminade , Steven D. Tanksley , Chenwei Lin
发明人： Maud Lepelley , James Gérard McCarthy , Vincent Petiard , Gerald Cheminade , Steven D. Tanksley , Chenwei Lin
CPC分类号： C12N15/8255 , C12N9/0006 , C12N9/0008 , C12N9/0071 , C12N9/0077 , C12N9/1007
摘要： Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignins in the coffee plant are disclosed. Also disclosed are methods for using these polynucleotides and polypeptides for the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation resistance to pathogen, herbivore, and insect attack in the coffee plant.
摘要翻译： 公开了编码包含咖啡植物中木质素生物合成途径的多肽的多核苷酸。 还公开了使用这些多核苷酸和多肽来操纵咖啡豆的风味，香味和其它特征的方法，以及对咖啡植物中对病原体，食草动物和昆虫攻击的操作抗性。
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8.发明授权公开(公告)号：US07989677B2
公开(公告)日：2011-08-02
申请号：US11990549
申请日：2006-08-16
申请人： Steven D. Tanksley , Chenwei Lin , Isabelle Muguette Privat , James Gérard McCarthy , Vincent Petiard
发明人： Steven D. Tanksley , Chenwei Lin , Isabelle Muguette Privat , James Gérard McCarthy , Vincent Petiard
CPC分类号： C12N15/8245
摘要： Disclosed herein are nucleic acid molecules isolated from coffee (Coffea spp.) comprising sequences that encodes various sucrose metabolizing enzymes, along with their encoded proteins. Specifically, sucrose synthase, sucrose phosphate synthase and sucrose phosphatase enzymes and their encoding polynucleotides from coffee are disclosed. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the sugar profile of coffee plants, to influence flavor, aroma, and other features of coffee beans.
摘要翻译： 本文公开了从包含编码各种蔗糖代谢酶的序列及其编码的蛋白质的咖啡（咖啡属）分离的核酸分子。 具体地，公开了蔗糖合酶，蔗糖磷酸合成酶和蔗糖磷酸酶及其来自咖啡的编码多核苷酸。 还公开了使用这些多核苷酸用于咖啡植物的糖型的基因调节和操作以影响咖啡豆的风味，香味和其它特征的方法。
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9.发明申请公开(公告)号：US20100154074A1
公开(公告)日：2010-06-17
申请号：US11992612
申请日：2006-09-27
CPC分类号： C12N9/2408 , C12N15/8243 , C12N15/8245 , C12N15/8261 , C12Y302/01026 , Y02A40/146
摘要： Disclosed herein are nucleic acid molecules isolated from coffee (Coffea spp.) comprising sequences that encodes various sucrose metabolizing enzymes, along with their encoded proteins. Specifically, three types of invertase and four invertase inhibitors and their encoding polynucleotides from coffee are disclosed. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the sugar profile of coffee plants, to influence flavor, aroma, and other features of coffee beans.
摘要翻译： 本文公开了从包含编码各种蔗糖代谢酶的序列及其编码的蛋白质的咖啡（咖啡属）分离的核酸分子。 具体地，公开了三种类型的转化酶和四种转化酶抑制剂及其来自咖啡的编码多核苷酸。 还公开了使用这些多核苷酸用于咖啡植物的糖型的基因调节和操作以影响咖啡豆的风味，香味和其它特征的方法。
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公开(公告)号：US08969541B2
公开(公告)日：2015-03-03
申请号：US13182213
申请日：2011-07-13
申请人： Isabelle M. Privat , James Gérard McCarthy , Vincent Petiard , Steven D. Tanksley , Chenwei Lin
发明人： Isabelle M. Privat , James Gérard McCarthy , Vincent Petiard , Steven D. Tanksley , Chenwei Lin
CPC分类号： C12N15/8245
摘要： Disclosed herein are nucleic acid molecules isolated from coffee (Coffea spp.) comprising sequences that encodes various sucrose metabolizing enzymes, along with their encoded proteins. Specifically, sucrose synthase, sucrose phosphate synthase and sucrose phosphatase enzymes and their encoding polynucleotides from coffee are disclosed. Also disclosed are methods for using these polynucleotides for gene regulation and manipulation of the sugar profile of coffee plants, to influence flavor, aroma, and other features of coffee beans.
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