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1.发明申请ENHANCEMENT OF BACILLUS THURINGIENSIS CRY PROTEIN TOXICITIES TO COLEOPTERANS, AND NOVEL INSECT CADHERIN FRAGMENTS 有权咖啡因增强蛋白质蛋白质对蛋鸡的毒性和新型内脏蛋白质片段公开(公告)号：US20110183896A1
公开(公告)日：2011-07-28
申请号：US12672974
申请日：2008-08-11
IPC分类号： A01N37/18 , C07K14/325 , A01P7/04 , A01P15/00
CPC分类号： A01N63/00 , A01N63/02 , A01N2300/00
摘要： The subject invention relates in part to the discovery that a fragment from a cadherin of the western corn rootworm enhances Cry3 toxicity to larvae of naturally susceptible species. The subject invention also relates in part to the discovery that a cadherin fragment from a beetle enhances Cry3Aa and Cry3Bb toxicity to coleopteran larvae, particularly those in the family Chrysomelidae. Such cadherin fragments are referred to as Bt Boosters (BtBs). The subject invention can be extended to the use of BtBs with other coleopteran-toxic Cry proteins for controlling a wide range of coleopterans.
摘要翻译： 本发明部分地涉及以下发现：玉米根虫西瓜的钙粘蛋白的片段增强对天然易感物种的幼虫的Cry3毒性。 本发明还部分涉及来自甲虫的钙粘着蛋白片段增强Cry3Aa和Cry3Bb对鞘翅目幼虫特别是家蝇科的鞘氨酯幼虫毒性的发现。 这种钙粘蛋白片段被称为Bt Boosters（BtBosts）（BtBs）。 本发明可以扩展到使用具有其它鞘翅目昆虫毒素Cry蛋白的BtB来控制广泛的鞘翅目。
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2.发明申请Novel Protein for Binding Bacillus Thuringiensis Cry Toxins and Fragments of Cadherins for Enhancing Cry Toxicity Against Dipterans 审中-公开用于结合苏云金芽孢杆菌的新型蛋白质用于增强对Dipterans的Cry毒性的卡介素的毒素和片段公开(公告)号：US20110112012A1
公开(公告)日：2011-05-12
申请号：US12936027
申请日：2009-04-03
IPC分类号： A01N37/18 , A01P7/04 , C07K14/435 , A01H5/00
CPC分类号： A01N63/02 , C07K14/43563 , C07K14/705 , C12N15/8286 , Y02A40/162 , A01N2300/00
摘要： The subject invention relates in part to a novel protein for binding Bacillus thuringiensis Cry toxins, and fragments of cadherins for enhancing Cry toxicity against dipterans. The subject invention also relates in part to the discovery that fragments of a midgut cadherin from a dipteran insect synergize Cry proteins that are active against dipterans. Thus, the subject invention includes the use of fragments of cadherin ectodomains for controlling dipterans. Such fragments (that bind Crys) can be administered to a dipteran insect for ingestion. In some preferred embodiments, the source cadherin is a dipteran cadherin. Also in some preferred embodiments, the fragment is administered with a Cry protein that is active against a dipteran. Variants of the fragments of naturally occurring cadherins are included within the scope of the subject invention.
摘要翻译： 本发明部分涉及用于结合苏云金芽孢杆菌Cry毒素的新型蛋白质和用于增强对dip子素的Cry毒性的钙粘蛋白片段。 本发明还部分涉及这样一个发现，即来自二极虫昆虫的中肠钙粘蛋白的片段与对dip虫有活性的Cry蛋白协同作用。 因此，本发明包括使用钙粘蛋白胞外域的片段来控制dip子。 可以将这样的片段（结合Crys）施用于用于摄取的dip虫。 在一些优选的实施方案中，源钙粘蛋白是二极体钙粘蛋白。 同样在一些优选的实施方案中，所述片段与对dipteran有活性的Cry蛋白施用。 天然存在的钙粘素的片段的变体包括在本发明的范围内。
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3.发明授权Enhancement of Bacillus thuringiensis Cry protein toxicities to coleopterans, and novel insect cadherin fragments 有权增强苏云金芽孢杆菌对鞘翅目的Cry蛋白毒性，以及新型昆虫钙粘蛋白片段公开(公告)号：US08486887B2
公开(公告)日：2013-07-16
申请号：US12672974
申请日：2008-08-11
CPC分类号： A01N63/00 , A01N63/02 , A01N2300/00
摘要： The subject invention relates in part to the discovery that a fragment from a cadherin of the western corn rootworm enhances Cry3 toxicity to larvae of naturally susceptible species. The subject invention also relates in part to the discovery that a cadherin fragment from a beetle enhances Cry3Aa and Cry3Bb toxicity to coleopteran larvae, particularly those in the family Chrysomelidae. Such cadherin fragments are referred to as Bt Boosters (BtBs). The subject invention can be extended to the use of BtBs with other coleopteran-toxic Cry proteins for controlling a wide range of coleopterans.
摘要翻译： 本发明部分地涉及以下发现：玉米根虫西瓜的钙粘蛋白的片段增强对天然易感物种的幼虫的Cry3毒性。 本发明还部分涉及来自甲虫的钙粘着蛋白片段增强Cry3Aa和Cry3Bb对鞘翅目幼虫特别是家蝇科的鞘氨酯幼虫毒性的发现。 这种钙粘蛋白片段被称为Bt Boosters（BtBosts）（BtBs）。 本发明可以扩展到使用具有其它鞘翅目昆虫毒素Cry蛋白的BtB来控制广泛的鞘翅目。
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公开(公告)号：US08101568B2
公开(公告)日：2012-01-24
申请号：US12163484
申请日：2008-06-27
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/705 , Y02A40/162 , A01N2300/00
摘要： The subject invention pertains to the use of peptide fragments of cadherins (including cadherin-like proteins). The subject invention includes a cell (and use thereof) comprising a polynucleotide that expresses the peptide fragment. The subject invention includes methods of feeding the peptides to insects. In preferred embodiments, the peptides are fed to target insects together with one or more insecticidal proteins, preferably (but not limited to) B.t. Cry proteins. When used in this manner, the peptide fragment can not only enhance the apparent toxin activity of the Cry protein against the insect species that was the source of the receptor but also against other insect species. Preferably, the cadherin is a Bacillus thuringiensis (B.t.) insecticidal crystal protein (Cry) toxin receptor. Preferably, the peptide fragment is a binding domain of the receptor. In some preferred embodiments, the peptide is the binding domain nearest to the membrane proximal ectodomain. Corresponding domains are identifiable in a variety of B.t. toxin receptors.
摘要翻译： 本发明涉及钙粘蛋白（包括钙粘蛋白样蛋白）的肽片段的用途。 本发明包括含有表达肽片段的多核苷酸的细胞（及其用途）。 本发明包括将肽喂入昆虫的方法。 在优选的实施方案中，将肽与一种或多种杀虫蛋白一起进食至靶昆虫，优选（但不限于）B.t. 哭泣蛋白质 当以这种方式使用时，肽片段不仅可以增强Cry蛋白对作为受体来源的昆虫物种的表观毒素活性，而且还可以抵抗其他昆虫物种。 优选地，钙粘蛋白是苏云金芽孢杆菌（B.t.）杀虫晶体蛋白（Cry）毒素受体。 优选地，肽片段是受体的结合结构域。 在一些优选的实施方案中，肽是最接近膜近端胞外域的结合结构域。 相应的域可以在各种B.t. 毒素受体。
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公开(公告)号：US09127076B2
公开(公告)日：2015-09-08
申请号：US12672972
申请日：2008-08-11
IPC分类号： C07K14/435 , A01N37/46 , C07K14/325
CPC分类号： C07K14/43563 , A01N37/46 , C07K14/325
摘要： This invention relates in part to modifying BtBooster (BtB) peptides, in part to increase their stability in insect midgut digestive juices. Some preferred embodiments of BtB have removed proteinase cleavage sites resulting in increased stability of the modified BtB in the insect gut, while retaining the ability to enhance B.t. proteins for improved insect control. In some preferred embodiments, the protease-stable BtB is used in combination with B.t. spores and/or crystals comprising a Cry protein. Also reported herein is the significant and increased enhancement of Bt toxins against relatively Bt-tolerant insects (Helicoverpa zea, Spodoptera exigua and Agrotis ipsilon), when used with BtBs. We also describe increased toxin enhancement with cadherin fragments that are stabilized against over-digestion by insect midgut proteinases. We also report enhancement of Bt Cry1F toxin by cadherin fragments.
摘要翻译： 本发明部分地涉及修饰BtBooster（BtB）肽，部分是为了增加它们在昆虫中肠消化液中的稳定性。 BtB的一些优选实施方案已经去除了蛋白酶切割位点，导致改良的BtB在昆虫肠中的稳定性增加，同时保留增强B.t.的能力。 用于改善昆虫控制的蛋白质。 在一些优选的实施方案中，蛋白酶稳定的BtB与B.t. 包含Cry蛋白的孢子和/或晶体。 本文还报道了当与BtB一起使用时，Bt毒素对相对耐受Bt的昆虫（Helicoverpa zea，Spodoptera exigua和Agrotis ipsilon）的显着和增加的增强。 我们还描述了通过昆虫中肠蛋白酶过度消化而稳定的钙粘蛋白片段增加的毒素增强。 我们还报告了钙粘蛋白片段对Bt Cry1F毒素的增强。
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公开(公告)号：US07396813B2
公开(公告)日：2008-07-08
申请号：US11040472
申请日：2005-01-21
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/705 , Y02A40/162 , A01N2300/00
摘要： The subject invention pertains to the use of peptide fragments of cadherins (including cadherin-like proteins). The subject invention includes a cell (and use thereof) comprising a polynucleotide that expresses the peptide fragment. The subject invention includes methods of feeding the peptides to insects. In preferred embodiments, the peptides are fed to target insects together with one or more insecticidal proteins, preferably (but not limited to) B.t. Cry proteins. When used in this manner, the peptide fragment can not only enhance the apparent toxin activity of the Cry protein against the insect species that was the source of the receptor but also against other insect species. Preferably, the cadherin is a Bacillus thuringiensis (B.t.) insecticidal crystal protein (Cry) toxin receptor. Preferably, the peptide fragment is a binding domain of the receptor. In some preferred embodiments, the peptide is the binding domain nearest to the membrane proximal ectodomain. Corresponding domains are identifiable in a variety of B.t. toxin receptors.
摘要翻译： 本发明涉及钙粘蛋白（包括钙粘蛋白样蛋白）的肽片段的用途。 本发明包括含有表达肽片段的多核苷酸的细胞（及其用途）。 本发明包括将肽喂入昆虫的方法。 在优选的实施方案中，将肽与一种或多种杀虫蛋白一起进食至靶昆虫，优选（但不限于）B.t. 哭泣蛋白质 当以这种方式使用时，肽片段不仅可以增强Cry蛋白对作为受体来源的昆虫物种的表观毒素活性，而且还可以抵抗其他昆虫物种。 优选地，钙粘蛋白是苏云金芽孢杆菌（B.t.）杀虫晶体蛋白（Cry）毒素受体。 优选地，肽片段是受体的结合结构域。 在一些优选的实施方案中，肽是最接近膜近端胞外域的结合结构域。 相应的域可以在各种B.t. 毒素受体。
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公开(公告)号：US20120220521A1
公开(公告)日：2012-08-30
申请号：US12672972
申请日：2008-08-11
IPC分类号： A01N37/46 , A01P7/04 , C12P21/02 , C07K14/435
CPC分类号： C07K14/43563 , A01N37/46 , C07K14/325
摘要： This invention relates in part to modifying BtBooster (BtB) peptides, in part to increase their stability in insect midgut digestive juices. Some preferred embodiments of BtB have removed proteinase cleavage sites resulting in increased stability of the modified BtB in the insect gut, while retaining the ability to enhance B.t. proteins for improved insect control. In some preferred embodiments, the protease-stable BtB is used in combination with B.t. spores and/or crystals comprising a Cry protein. Also reported herein is the significant and increased enhancement of Bt toxins against relatively Bt-tolerant insects (Helicoverpa zea, Spodoptera exigua and Agrotis ipsilon), when used with BtBs. We also describe increased toxin enhancement with cadherin fragments that are stabilized against over-digestion by insect midgut proteinases. We also report enhancement of Bt Cry1F toxin by cadherin fragments.
摘要翻译： 本发明部分地涉及修饰BtBooster（BtB）肽，部分是为了增加它们在昆虫中肠消化液中的稳定性。 BtB的一些优选实施方案已经去除了蛋白酶切割位点，导致改良的BtB在昆虫肠中的稳定性增加，同时保留增强B.t.的能力。 用于改善昆虫控制的蛋白质。 在一些优选的实施方案中，蛋白酶稳定的BtB与B.t. 包含Cry蛋白的孢子和/或晶体。 本文还报道了当与BtB一起使用时，Bt毒素对相对耐受Bt的昆虫（Helicoverpa zea，Spodoptera exigua和Agrotis ipsilon）的显着和增加的增强。 我们还描述了通过昆虫中肠蛋白酶过度消化而稳定的钙粘蛋白片段增加的毒素增强。 我们还报告了钙粘蛋白片段对Bt Cry1F毒素的增强。
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公开(公告)号：US20090018075A1
公开(公告)日：2009-01-15
申请号：US12163508
申请日：2008-06-27
IPC分类号： A01N61/00 , C07K14/325 , A01P7/04
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/705 , Y02A40/162 , A01N2300/00
摘要： The subject invention pertains to the use of peptide fragments of cadherins (including cadherin-like proteins). The subject invention includes a cell (and use thereof) comprising a polynucleotide that expresses the peptide fragment. The subject invention includes methods of feeding the peptides to insects. In preferred embodiments, the peptides are fed to target insects together with one or more insecticidal proteins, preferably (but not limited to) B.t. Cry proteins. When used in this manner, the peptide fragment can not only enhance the apparent toxin activity of the Cry protein against the insect species that was the source of the receptor but also against other insect species. Preferably, the cadherin is a Bacillus thuringiensis (B.t.) insecticidal crystal protein (Cry) toxin receptor. Preferably, the peptide fragment is a binding domain of the receptor. In some preferred embodiments, the peptide is the binding domain nearest to the membrane proximal ectodomain. Corresponding domains are identifiable in a variety of B.t. toxin receptors.
摘要翻译： 本发明涉及钙粘蛋白（包括钙粘蛋白样蛋白）的肽片段的用途。 本发明包括含有表达肽片段的多核苷酸的细胞（及其用途）。 本发明包括将肽喂入昆虫的方法。 在优选的实施方案中，将肽与一种或多种杀虫蛋白一起进食至靶昆虫，优选（但不限于）B.t. 哭泣蛋白质 当以这种方式使用时，肽片段不仅可以增强Cry蛋白对作为受体来源的昆虫物种的表观毒素活性，而且还可以抵抗其他昆虫物种。 优选地，钙粘蛋白是苏云金芽孢杆菌（B.t.）杀虫晶体蛋白（Cry）毒素受体。 优选地，肽片段是受体的结合结构域。 在一些优选的实施方案中，肽是最接近膜近端胞外域的结合结构域。 相应的域可以在各种B.t. 毒素受体。
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公开(公告)号：US20090018074A1
公开(公告)日：2009-01-15
申请号：US12163484
申请日：2008-06-27
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/705 , Y02A40/162 , A01N2300/00
摘要： The subject invention pertains to the use of peptide fragments of cadherins (including cadherin-like proteins). The subject invention includes a cell (and use thereof) comprising a polynucleotide that expresses the peptide fragment. The subject invention includes methods of feeding the peptides to insects. In preferred embodiments, the peptides are fed to target insects together with one or more insecticidal proteins, preferably (but not limited to) B.t. Cry proteins. When used in this manner, the peptide fragment can not only enhance the apparent toxin activity of the Cry protein against the insect species that was the source of the receptor but also against other insect species. Preferably, the cadherin is a Bacillus thuringiensis (B.t.) insecticidal crystal protein (Cry) toxin receptor. Preferably, the peptide fragment is a binding domain of the receptor. In some preferred embodiments, the peptide is the binding domain nearest to the membrane proximal ectodomain. Corresponding domains are identifiable in a variety of B.t. toxin receptors.
摘要翻译： 本发明涉及钙粘蛋白（包括钙粘蛋白样蛋白）的肽片段的用途。 本发明包括含有表达肽片段的多核苷酸的细胞（及其用途）。 本发明包括将肽喂入昆虫的方法。 在优选的实施方案中，将肽与一种或多种杀虫蛋白一起进食至靶昆虫，优选（但不限于）B.t. 哭泣蛋白质 当以这种方式使用时，肽片段不仅可以增强Cry蛋白对作为受体来源的昆虫物种的表观毒素活性，而且还可以抵抗其他昆虫物种。 优选地，钙粘蛋白是苏云金芽孢杆菌（B.t.）杀虫晶体蛋白（Cry）毒素受体。 优选地，肽片段是受体的结合结构域。 在一些优选的实施方案中，肽是最接近膜近端胞外域的结合结构域。 相应的域可以在各种B.t. 毒素受体。
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