公开(公告)号：US20120276562A1

公开(公告)日：2012-11-01

申请号：US13502198

申请日：2010-10-13

申请人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

发明人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

IPC分类号： G01N33/566 ,  C07K16/00 ,  G01N21/47 ,  G01N33/545 ,  G01N21/78

CPC分类号： G01N33/6893 ,  C07K16/36 ,  C07K2317/34 ,  G01N33/5308 ,  G01N2333/75 ,  G01N2800/08

摘要： Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.

摘要翻译： 公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定由于人纤维蛋白原的α-E链和α链的降解而产生的并用作诊断肝脏疾病的肽标志物的5.9kDa肽 通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。

公开(公告)号：US09017959B2

公开(公告)日：2015-04-28

申请号：US13502198

申请日：2010-10-13

申请人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

发明人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  C07K16/36

CPC分类号： G01N33/6893 ,  C07K16/36 ,  C07K2317/34 ,  G01N33/5308 ,  G01N2333/75 ,  G01N2800/08

摘要： Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.

摘要翻译： 公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定由于人纤维蛋白原的α-E链和α链的降解而产生的并用作诊断肝脏疾病的肽标志物的5.9kDa肽 通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。