公开(公告)号：US20120252031A1

公开(公告)日：2012-10-04

申请号：US13479167

申请日：2012-05-23

申请人： John C. Herr ,  Monika Sachdev ,  Arabinda Mandal

发明人： John C. Herr ,  Monika Sachdev ,  Arabinda Mandal

IPC分类号： C12Q1/37 ,  G01N33/573

CPC分类号： C07K16/28 ,  A61K38/1709 ,  A61K38/177 ,  A61K38/4886 ,  A61K39/0006 ,  C07K2317/76 ,  G01N2333/96486 ,  G01N2500/04

摘要： The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.

摘要翻译： 本发明提供了用于调节受精和用作基于本发明的卵母细胞特异性蛋白质SAS1R（精子顶体SLLP1受体）的组合物和方法，其是精子蛋白受体。 确定了六种SAS1R变体，包括全长SAS1R。 mSLLP1和SAS1R共同定位于卵母细胞和顶体反应精子的顶体。 通过远西分析，在严格选择条件下的酵母双杂交系统中，通过抗mSLLP1和相反的免疫沉淀法证明了mSLLP1和SAS1R之间的相互作用。 发现纯化的重组SAS1R具有蛋白酶活性，在体外抑制受精，并在雌性中诱导免疫应答。 结果表明，SAS1R是精子顶体内配体SLLP1的蛋白水解活性，卵母细胞和早期胚胎特异性卵母细胞金属蛋白酶受体，是调节受精和避孕的目标。

公开(公告)号：US09803012B2

公开(公告)日：2017-10-31

申请号：US13479167

申请日：2012-05-23

申请人： John C. Herr ,  Monika Sachdev ,  Arabinda Mandal

发明人： John C. Herr ,  Monika Sachdev ,  Arabinda Mandal

IPC分类号： C07K16/28 ,  A61K38/17 ,  A61K38/48 ,  A61K39/00

CPC分类号： C07K16/28 ,  A61K38/1709 ,  A61K38/177 ,  A61K38/4886 ,  A61K39/0006 ,  C07K2317/76 ,  G01N2333/96486 ,  G01N2500/04

摘要： The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.

公开(公告)号：US20100183617A1

公开(公告)日：2010-07-22

申请号：US12613947

申请日：2009-11-06

申请人： John C. Herr ,  Monika Sachdev ,  Arabinda Mandal

发明人： John C. Herr ,  Monika Sachdev ,  Arabinda Mandal

IPC分类号： A61K39/395 ,  G01N33/567 ,  C07K16/18 ,  A61K39/00 ,  A61K38/46

CPC分类号： C07K16/28 ,  A61K38/1709 ,  A61K38/177 ,  A61K38/4886 ,  A61K39/0006 ,  C07K2317/76 ,  G01N2333/96486 ,  G01N2500/04

摘要： The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.

摘要翻译： 本发明提供了用于调节受精和用作基于本发明的卵母细胞特异性蛋白质SAS1R（精子顶体SLLP1受体）的组合物和方法，其是精子蛋白受体。 确定了六种SAS1R变体，包括全长SAS1R。 mSLLP1和SAS1R共同定位于卵母细胞和顶体反应精子的顶体。 通过远西分析，在严格选择条件下的酵母双杂交系统中，通过抗mSLLP1和相反的免疫沉淀法证明了mSLLP1和SAS1R之间的相互作用。 发现纯化的重组SAS1R具有蛋白酶活性，在体外抑制受精，并在雌性中诱导免疫应答。 结果表明，SAS1R是精子顶体内配体SLLP1的蛋白水解活性，卵母细胞和早期胚胎特异性卵母细胞金属蛋白酶受体，是调节受精和避孕的目标。

公开(公告)号：US20090214552A1

公开(公告)日：2009-08-27

申请号：US11915225

申请日：2006-02-21

申请人： Arabinda Mandal ,  Monika Sachdev ,  John C. Herr

发明人： Arabinda Mandal ,  Monika Sachdev ,  John C. Herr

IPC分类号： A61K39/395 ,  A61K31/711 ,  A61K38/17 ,  A61P15/18

CPC分类号： C12N9/6421 ,  C07K14/705

摘要： The present invention relates to novel egg membrane protein receptors and to methods of inhibiting the interaction of sperm proteins with egg proteins. The invention further relates to methods of preventing and inhibiting sperm-egg binding, sperm-egg fusion, and fertilization. The invention further relates to the egg membrane proteins MET and ZEP.

摘要翻译： 本发明涉及新型卵膜蛋白受体以及抑制精蛋白与卵蛋白相互作用的方法。 本发明还涉及预防和抑制精子卵结合，精卵融合和受精的方法。 本发明还涉及蛋膜蛋白质MET和ZEP。