公开(公告)号：US20170355940A1

公开(公告)日：2017-12-14

申请号：US15527222

申请日：2015-11-19

申请人： NORTIS, INC.

发明人： Thomas Neumann ,  Mark E. Fauver ,  Richard Carleton Hulit

IPC分类号： C12M3/04 ,  C12M3/06 ,  C12M3/00 ,  A61F2/06

CPC分类号： C12M3/04 ,  A61F2/06 ,  C12M21/08 ,  C12M23/16 ,  C12N5/0691 ,  C12N5/0697 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2502/22 ,  C12N2502/28 ,  C12N2502/30

摘要： Vascularizing cell aggregates or tissue segments in a microfluidic device by filling a chamber within the device with a matrix that allows for endothelial sprouting; creating at least three voids within the matrix, of which at least two outer voids are lumenally connected to separate perfusion paths within the device and at least one additional void is positioned in between the at least two outer voids; endothelializing the at least two outer voids; introducing at least one cell type, matrix material, tissue segment, or combinations thereof into the void between the two outer voids; and using vascular growth factors to induce the endothelial cells to sprout into the matrix until the at least three voids are interconnected by endothelial sprouts.

公开(公告)号：US20190062684A1

公开(公告)日：2019-02-28

申请号：US16025806

申请日：2018-07-02

申请人： NORTIS, INC.

发明人： Thomas Neumann ,  Anna A. Tourovskaia ,  Mark E. Fauver ,  Greg Kramer ,  Elizabeth McClure ,  Henning Mann

IPC分类号： C12M3/00 ,  G01N33/50 ,  C12M1/12 ,  C12M1/00 ,  C12M1/26 ,  C12M3/06 ,  A01N1/02

摘要： A microfluidic system including a number of microfluidic devices having a first perfusion path and a second separate perfusion path; the microfluidic devices each also having a chamber containing a matrix, where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path, where the at least one void is populated with at least one cell type in such way that the cells are in direct contact with the matrix; where the matrix is in fluidic connection exclusively with the second separate perfusion path. The microfluidic devices are integrated onto a platform; and each of the microfluidic devices mimics at least a partial organ module.

公开(公告)号：US20150240194A1

公开(公告)日：2015-08-27

申请号：US14431015

申请日：2013-09-27

申请人： NORTIS, INC.

发明人： Thomas Neumann ,  Anna A. Tourovskaia ,  Mark E. Fauver ,  Greg Kramer ,  Elizabeth Asp ,  Henning Mann

IPC分类号： C12M3/00 ,  G01N33/50 ,  C12M3/06 ,  C12M1/26 ,  C12M1/12 ,  C12M1/00

CPC分类号： C12M21/08 ,  A01N1/0231 ,  A01N1/0247 ,  C12M23/16 ,  C12M25/14 ,  C12M29/06 ,  C12M29/10 ,  C12M33/00 ,  C12M33/04 ,  G01N33/5085

摘要： A microfluidic system for generating compartmentalized microenvironments of tissues and organs in vitro and for independently perfusing the compartments. A microfluidic device that includes at least a first perfusion path and a second separate perfusion path. The microfluidic device also has a chamber containing a matrix, where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path, where the at least one void can be populated with at least one cell type in such way that the cells are in direct contact with the matrix and the matrix is in fluidic connection exclusively with the second separate perfusion path.

摘要翻译： 用于在体外产生组织和器官的分隔的微环境并用于独立灌注隔室的微流体系统。 一种微流体装置，其包括至少第一灌注路径和第二分离灌注路径。 所述微流体装置还具有包含基质的腔室，其中所述基质包围至少一个空腔，所述至少一个空腔的内腔与第一灌注路径专用流体连接，其中至少一个空隙可以以这种方式填充至少一种细胞类型 细胞与基质直接接触，并且基质仅与第二分离灌注路径流体连接。