摘要:
The invention relates to a medium for storing isolated pancreatic islets and a method for storing isolated pancreatic islets. Such a medium is suitable for storing pancreatic islets both at physiological temperature and refrigerated.
摘要:
The present disclosure provides a method for freeze-drying cells in a hydrogel comprising nanofibrillar cellulose, the method comprising providing a hydrogel comprising nanofibrillar cellulose, providing cells, combining the cells and the hydrogel comprising nanofibrillar cellulose to form a cell system, and freeze drying the cell system to obtain dried cells in a hydrogel comprising nanofibrillar cellulose. The present disclosure also provides a freeze-dried hydrogel comprising nanofibrillar cellulose and cells.
摘要:
Systems and methods are disclosed relating to extracorporeal fetal care. A fetal chamber assembly configured to enclose and support a fetus therein includes a base configured to receive the fetus therein; a lid configured to removably contact the base to form a liquid-tight seal between the lid and the base; a growth chamber defined between the base and the lid, the growth chamber being configured to receive the fetus therein; and a cannulation chamber in fluid communication with the growth chamber, the cannulation chamber being configured to receive therein a cannulated umbilical cord of the fetus. The growth chamber is configured to be adjusted in size to accommodate the fetus during gestation based on the size of the fetus, and the fetal chamber assembly is configured to receive a liquid from a liquid source.
摘要:
A method for cryopreservation of adherent cells attached to a substrate and a method for identifying one or more extracellular matrix (ECM) components and/or matricellular proteins that improves viability and retention of the cryopreserved cells on the substrate. The method for cryopreservation includes treating a substrate with at least one ECM component and/or adding at least one matricellular protein to the substrate or cell culture media, plating the cells on the treated substrate, and cryopreserving the cells. One or more ECM components and/or matricellular proteins that improves cell viability and retention can be identified by evaluating the cells that have been thawed from the cryopreservation temperature to determine cell viability and retention.
摘要:
The present invention relates generally to systems and methods for storing, shipping and using skin equivalents made by organotypic culture. In particular, the present invention relates to systems and methods for producing, transporting, storing and using skin equivalents produced by organotypic culture at reduced temperatures, preferably from 2-8 degrees Celsius. The methods include sterile packaging of the grafts so that the sterility and integrity of the package is maintained until the time of use for grafting purposes.
摘要:
The present invention provides a method for cryoprotecting a biological specimen comprising the step of freezing said biological specimen in the presence of a hydrogel and in the absence of cryoprotectant.
摘要:
This disclosure is in the field of medical therapy; in particular, it concerns the use of encapsulated cells in cell therapy. More in particular, this disclosure relates to the second medical use of a composition comprising encapsulated cells. Even more in particular, the disclosure relates to the use of a foreign body, suitable for implantation into a subject at a predefined location, wherein the foreign body comprises cells encapsulated in high-M alginate for inducing or stimulating angiogenesis.
摘要:
A method is for the cryopreservation of a (plant) cell, (plant) organelle or (plant) tissue, where the (plant) cell, (plant) organelle or (plant) tissue is encapsulated in a matrix formed of an abiotic material in presence of one or more additives with cryoprotectant effect. The method provides a faster, more effective and cheaper way to cryopreserve biological material.
摘要:
A microfluidic system for generating compartmentalized microenvironments of tissues and organs in vitro and for independently perfusing the compartments. A microfluidic device that includes at least a first perfusion path and a second separate perfusion path. The microfluidic device also has a chamber containing a matrix, where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path, where the at least one void can be populated with at least one cell type in such way that the cells are in direct contact with the matrix and the matrix is in fluidic connection exclusively with the second separate perfusion path.
摘要:
The present invention provides a method for cryoprotecting a biological specimen comprising the step of freezing said biological specimen in the presence of a hydrogel and in the absence of cryoprotectant.