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1.发明申请Transcriptional regulatory elements of biological pathways tools, and methods 审中-公开转录调控因子的生物学途径工具和方法公开(公告)号：US20090018031A1
公开(公告)日：2009-01-15
申请号：US11999792
申请日：2007-12-06
CPC分类号： C12N15/1086 , C12N15/1051 , C12N15/1089 , C40B40/06
摘要： The present invention provides compositions, kits, assemblies, libraries, arrays, and high throughput methods for large scale structural and functional characterization of gene expression regulatory elements in a genome of an organism, especially in a human genome, that are part of a common pathway. In one aspect of the invention, an array of expression constructs is provided, each of the expression constructs comprising: a nucleic acid segment operably linked with a reporter sequence in an expression vector such that expression of the reporter sequence is under the transcriptional control of the nucleic acid segment. The present invention can have a wide variety of applications such as in personalized medicine, pharmacogenomics, and correlation of polymorphisms with phenotypic traits.
摘要翻译： 本发明提供组合物，试剂盒，组装，文库，阵列和高通量方法，用于大规模结构和功能表征基因表达调控元件，所述基因表达调控元件是生物体，特别是在人类基因组中的基因表达调控元件，其是共同途径的一部分 。 在本发明的一个方面，提供了表达构建体的阵列，每个表达构建体包含：与表达载体中的报道序列可操作地连接的核酸片段，使得报告序列的表达在 核酸片段。 本发明可以具有广泛的应用，例如个性化医学，药物基因组学和多态性与表型性状的相关性。
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2.发明申请Functional arrays for high throughput characterization of regulatory elements in untranslated regions of genes 审中-公开用于基因非翻译区调节元件高通量表征的功能阵列公开(公告)号：US20080220983A1
公开(公告)日：2008-09-11
申请号：US12074856
申请日：2008-03-05
CPC分类号： C12N15/1086 , C12N15/67 , C12Q1/6897
摘要： This invention provides libraries of expression constructs having different untranslated regions (UTR) from a genome. The expression constructs include transcription regulatory sequences operably linked with a reporter gene and a 5′ UTR, a 3′ UTR or both, wherein the translation of the reporter gene is under the regulatory control of control regions in the UTR sequence. The libraries of this invention are useful for determining the impact of regulatory sequences in the UTRs on translation of open reading frames under a variety of conditions, such as different cellular environments.
摘要翻译： 本发明提供了具有来自基因组的不同非翻译区（UTR）的表达构建体的文库。 表达构建体包括与报告基因和5'UTR，3'UTR或两者可操作地连接的转录调节序列，其中报告基因的翻译在UTR序列中的对照区域的调节控制下。 本发明的文库可用于确定在各种条件下诸如不同细胞环境的UTRs中调节序列对开放阅读框的翻译的影响。
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3.发明申请Functional arrays for high throughput characterization of gene expression regulatory elements 审中-公开用于基因表达调节元件高通量表征的功能阵列公开(公告)号：US20070161031A1
公开(公告)日：2007-07-12
申请号：US11636385
申请日：2006-12-07
申请人： Nathan Trinklein , Shelley Aldred , Sara Cooper , Richard Myers
发明人： Nathan Trinklein , Shelley Aldred , Sara Cooper , Richard Myers
CPC分类号： C12N15/1086 , C12Q1/6897
摘要： The present invention provides compositions, kits, assemblies, libraries, arrays, and high throughput methods for large scale structural and functional characterization of gene expression regulatory elements in a genome of an organism, especially in a human genome. In one aspect of the invention, an array of expression constructs is provided, each of the expression constructs comprising: a nucleic acid segment operably linked with a reporter sequence in an expression vector such that expression of the reporter sequence is under the transcriptional control of the nucleic acid segment, the nucleic acid segment varying in the library and having a diversity of at least 50. The nucleic acid segments can be a large library of gene expression regulatory elements such as transcriptional promoters. The present invention can have a wide variety of applications such as in personalized medicine, pharmacogenomics, and correlation of polymorphisms with phenotypic traits.
摘要翻译： 本发明提供组合物，试剂盒，组装，文库，阵列和高通量方法，用于在生物体，特别是人类基因组的基因组中的基因表达调控元件的大规模结构和功能表征。 在本发明的一个方面，提供了表达构建体的阵列，每个表达构建体包含：与表达载体中的报道序列可操作地连接的核酸片段，使得报告序列的表达在 核酸片段，核酸片段在文库中变化并具有至少50的多样性。核酸片段可以是基因表达调节元件如转录启动子的大型文库。 本发明可以具有广泛的应用，例如个性化医学，药物基因组学和多态性与表型性状的相关性。
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