公开(公告)号：US20070212786A1

公开(公告)日：2007-09-13

申请号：US11540695

申请日：2006-09-29

申请人： Scott Manalis ,  Tzu Loh ,  Michel Godin ,  Nebojsa Milovic ,  Jonathan Behr ,  Aarthi Chandrasekaran ,  Ram Sasisekharan

发明人： Scott Manalis ,  Tzu Loh ,  Michel Godin ,  Nebojsa Milovic ,  Jonathan Behr ,  Aarthi Chandrasekaran ,  Ram Sasisekharan

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  B01L3/5027 ,  G01N27/4145 ,  G01N2333/8128

摘要： In one aspect, the present invention provides a device and method for real-time, direct detection of heparin in buffer and in serum comprising a microfluidic field-effect device as an affinity biosensor. The sensor is based on an electrolyte-insulator-silicon structure, and is manufactured by a standard high-yield silicon microfabrication process. The binding of heparin to the sensor surface induces a change in the insulator-electrolyte surface potential, which is measured as a change in sensor capacitance. To ensure the binding selectivity, protamine and antithrombin III are used as affinity probes.

摘要翻译： 一方面，本发明提供了用于在缓冲液和血清中实时，直接检测肝素的装置和方法，其包括作为亲和生物传感器的微流场效应装置。 该传感器基于电解质 - 绝缘体 - 硅结构，并通过标准的高产率硅微细加工工艺制造。 肝素与传感器表面的结合导致绝缘体 - 电解质表面电位的变化，其被测量为传感器电容的变化。 为了确保结合选择性，使用鱼精蛋白和抗凝血酶III作为亲和探针。