公开(公告)号：US20240035014A1

公开(公告)日：2024-02-01

申请号：US18485895

申请日：2023-10-12

Applicant: Nicholas Stephanopoulos ,  Yang Xu

Inventor： Nicholas Stephanopoulos ,  Yang Xu

IPC: C12N9/88 ,  C07K19/00 ,  B82Y5/00 ,  C12N15/11

CPC classification number: C12N9/88 ,  C07K19/00 ,  C12Y401/02014 ,  B82Y5/00 ,  C12N15/11

Abstract: Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.

公开(公告)号：US20230053360A1

公开(公告)日：2023-02-23

申请号：US17818856

申请日：2022-08-10

Applicant: Nicholas Stephanopoulos ,  Tara MacCulloch

Inventor： Nicholas Stephanopoulos ,  Tara MacCulloch

IPC: A61K47/69 ,  C12N15/113 ,  A61K47/64 ,  A61K47/54

Abstract: The present disclosure relates to nucleic acid-peptide-nucleic acid conjugate molecules and to methods for synthesizing nucleic acid-peptide-nucleic acid conjugate molecules. In some embodiments, a method for synthesizing a nucleic acid-peptide-nucleic acid conjugate molecule using proximity-enhanced synthesis includes covalently linking a peptide with a first nucleic acid strand via a first reaction, hybridizing the first nucleic acid strand with a second nucleic acid strand to bring the second nucleic acid strand in proximity to the peptide, and covalently linking the peptide with the second nucleic acid strand via a second reaction to provide the nucleic acid-peptide-nucleic acid conjugate molecule. In some embodiments, the peptide of the nucleic acid-peptide-nucleic acid conjugate molecule is a substrate for cleavage by an enzyme, such as matrix metalloproteinase-8 (MMP-8). Exemplary applications of the nucleic acid-peptide-nucleic acid conjugate molecule for drug delivery, molecular assembly of hybrid structures, and constraining the peptide to a biologically active conformation are also disclosed.

公开(公告)号：US20200289658A1

公开(公告)日：2020-09-17

申请号：US16816029

申请日：2020-03-11

Applicant: Nicholas Stephanopoulos ,  Yang Xu

Inventor： Nicholas Stephanopoulos ,  Yang Xu

IPC: A61K47/64 ,  C12N7/00 ,  A61K47/69 ,  C12N9/96

Abstract: Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.

公开(公告)号：US11814659B2

公开(公告)日：2023-11-14

申请号：US16816029

申请日：2020-03-11

Applicant: Nicholas Stephanopoulos ,  Yang Xu

Inventor： Nicholas Stephanopoulos ,  Yang Xu

IPC: C12N9/88 ,  C07K19/00 ,  B82Y5/00 ,  C12N15/11

CPC classification number: C12N9/88 ,  B82Y5/00 ,  C07K19/00 ,  C12N15/11 ,  C12Y401/02014

Abstract: Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.

公开(公告)号：US20230181761A1

公开(公告)日：2023-06-15

申请号：US18063824

申请日：2022-12-09

Applicant: Nicholas Stephanopoulos

Inventor： Nicholas Stephanopoulos

IPC: A61K47/69 ,  A61K47/62

CPC classification number: A61K47/6929 ,  A61K47/62 ,  B82Y5/00

Abstract: Described herein are DNA nanostructures (DN) functionalized with proteins and methods for cellular uptake. Cellular uptake of such DNs is linearly dependent on the cell size. The protein corona determines the endolysosomal vesicle escape efficiency of DNs coated with an endosome escape peptide.