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公开(公告)号：US08617560B2
公开(公告)日：2013-12-31
申请号：US11391522
申请日：2006-03-28
申请人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
发明人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
CPC分类号： C07K14/005 , A61K39/00 , C12N2710/16122 , C12N2710/16134 , Y02P20/55
摘要： The present invention relates to peptides or pretide derivatives of the 65kD lower matrix phosphoprotein of human cytomegalovirus. The peptides disclosed are useful for determining whether subjects have een exposed to human cytomegalovirus. Methods for using these peptides or derivatives thereof to determine the peptide-induced production of interferon-γ and/or TNF-α in CD8+ T cells isolated from subjects are also disclosed.
摘要翻译： 本发明涉及人巨细胞病毒的65kD下基质磷蛋白的肽或衍生物衍生物。 所公开的肽可用于确定受试者是否暴露于人巨细胞病毒。 还公开了使用这些肽或其衍生物来测定从受试者分离的CD8 + T细胞中肽诱导的干扰素-γ和/或TNF-α产生的方法。
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公开(公告)号：US20060165714A1
公开(公告)日：2006-07-27
申请号：US11391522
申请日：2006-03-28
申请人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
发明人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
IPC分类号： A61K39/12 , C12Q1/70 , C07K7/08 , C07K14/005
CPC分类号： C07K14/005 , A61K39/00 , C12N2710/16122 , C12N2710/16134 , Y02P20/55
摘要： The present invention relates to peptides or pretide derivatives of the 65 kD lower matrix phosphoprotein of human cytomegalovirus. The peptides disclosed are useful for determining whether subjects have een exposed to human cytomegalovirus. Methods for using these peptides or derivatives thereof to determine the peptide-induced production of interferon-γ and/or TNF-α in CD8+ T cells isolated from subjects are also disclosed.
摘要翻译： 本发明涉及人巨细胞病毒的65kD下基质磷蛋白的肽或预制衍生物。 所公开的肽可用于确定受试者是否暴露于人巨细胞病毒。 还公开了使用这些肽或其衍生物来确定从受试者分离的CD8 + T细胞中肽诱导的干扰素-γ和/或TNF-α产生的方法。
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公开(公告)号：US07041442B1
公开(公告)日：2006-05-09
申请号：US09980058
申请日：2000-06-02
申请人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
发明人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
IPC分类号： C12Q1/70 , G01N33/567 , C07K4/02 , C12N15/38
CPC分类号： C07K14/005 , A61K39/00 , C12N2710/16122 , C12N2710/16134 , Y02P20/55
摘要： The present invention relates to peptides or peptide derivatives of the 65 kD lower matrix phoshpoprotein of human cytomegalovirus. The peptides disclosed are useful for determining whether subjects have been exposed to human cytomegalovirus. Methods for using these peptides or derivatives thereof to determine the peptide-induced production of interferon-γ and/or TNF-α in CD8+ T cells isolated from subjects are also disclosed.
摘要翻译： 本发明涉及人巨细胞病毒的65kD下基质磷酸蛋白的肽或肽衍生物。 所公开的肽可用于确定受试者是否已暴露于人巨细胞病毒。 还公开了使用这些肽或其衍生物来确定从受试者分离的CD8 + T细胞中肽诱导的干扰素-γ和/或TNF-α产生的方法。
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4.发明授权Method for antigen-specific stimulation of T lymphocytes with synthetic peptide libraries 有权用合成肽文库对T淋巴细胞进行抗原特异性刺激的方法公开(公告)号：US07994096B2
公开(公告)日：2011-08-09
申请号：US10203915
申请日：2001-02-17
申请人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
发明人： Florian Kern , Hans-Dieter Volk , Petra Reinke , Nicole Faulhaber , Ingolf-Pascal Surel , Elham Khatamzas
CPC分类号： G01N33/505
摘要： The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.
摘要翻译： 本发明涉及一种用合成肽文库抗原特异性刺激T淋巴细胞的方法，通过制备多种肽，所述肽中的每一种都包含小于整个抗原总氨基酸序列的片段， 所述肽中的每一个长度为至少9个氨基酸残基（AAs），并且每个肽的氨基酸序列与所述肽中的至少另一个的氨基酸序列重叠; 将来自a）的多个肽组合成肽文库，所述肽文库包含多个选择的肽，使得它们的共同重叠氨基酸序列跨越抗原的总氨基酸序列; 并在单次培养运行中将包含CD8 + T淋巴细胞，CD4 + T淋巴细胞或CD8 +和CD4 +的混合物的悬浮液与所述肽文库一起孵育。
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