公开(公告)号：US06943022B2

公开(公告)日：2005-09-13

申请号：US10245813

申请日：2002-09-17

申请人： Gyun Min Lee ,  No Soo Kim

发明人： Gyun Min Lee ,  No Soo Kim

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K48/00 ,  A61P43/00 ,  C12N15/113 ,  C12N15/57 ,  C12N5/00 ,  C07H21/04

CPC分类号： C12N15/1137 ,  A61K38/00 ,  C12N2310/111 ,  C12Y304/22056

摘要： The present invention relates to an antisense nucleotide of caspase-3, an expression vector encoding antisense RNA of caspase-3, and an inhibition method of cellular apoptosis in recombinant Chinese hamster ovary (CHO) cells using the same. More precisely, the present invention relates to an antisense nucleotide composed of a base sequence represented by Sequence ID No. 1, which suppresses the expression of caspase-3, an expression vector containing the above antisense nucleotide and expressing antisense RNA of caspase-3, and an inhibition method of apoptosis of recombinant cells by suppressing the expression of caspase-3 through introducing the above expression vector into the recombinant cells and then expressing antisense RNA of caspase-3. According to the present invention, apoptosis can be inhibited by suppressing the activation of caspase-3, which is related to the apoptosis, and the integrity of target protein produced in the recombinant cells can be enhanced.

摘要翻译： 本发明涉及半胱天冬酶-3的反义核苷酸，编码半胱天冬酶-3的反义RNA的表达载体，以及使用其的重组中国仓鼠卵巢（CHO）细胞中的细胞凋亡的抑制方法。 更准确地说，本发明涉及由序列号1表示的碱基序列构成的反义核苷酸，其抑制半胱天冬酶-3的表达，含有上述反义核苷酸并表达半胱天冬酶-3的反义RNA的表达载体， 以及通过将上述表达载体导入重组细胞中，然后表达半胱天冬酶-3的反义RNA，通过抑制半胱天冬酶-3的表达来抑制重组细胞凋亡的抑制方法。 根据本发明，通过抑制与凋亡相关的半胱天冬酶-3的活化，可以抑制细胞凋亡，能够提高重组细胞中产生的靶蛋白的完整性。