公开(公告)号：US08187873B2

公开(公告)日：2012-05-29

申请号：US12375210

申请日：2007-07-27

申请人： Goro Kominami ,  Masahiro Nakamura ,  Noriaki Kume ,  Hideki Ohta

发明人： Goro Kominami ,  Masahiro Nakamura ,  Noriaki Kume ,  Hideki Ohta

IPC分类号： C12N5/07 ,  C07K16/00

CPC分类号： C07K16/28 ,  C07K2317/92

摘要： It is intended to provide a monoclonal antibody that specifically recognizes human soluble LOX-1, particularly a monoclonal antibody with a dissociation constant (Kd) for human soluble LOX-1 of 1×10−9 (M) or less. The antibody can be produced from a hybridoma prepared by a method including the following steps of: (1) immunizing a non-human animal with a prokaryotic cell-derived human LOX-1 extracellular domain; (2) collecting antibody-producing cells from the animal; (3) fusing the antibody-producing cells with myeloma cells; (4) selecting hybridomas that produce a monoclonal antibody reacting with the human LOX-1 extracellular domain from the fused cells obtained in the above step; and (5) selecting a hybridoma that produces a monoclonal antibody reacting with a eukaryotic cell-derived human LOX-1 extracellular domain from the selected hybridomas.

摘要翻译： 旨在提供特异性识别人可溶性LOX-1的单克隆抗体，特别是具有1×10-9（M）以下的人可溶性LOX-1的解离常数（Kd）的单克隆抗体。 抗体可以由通过包括以下步骤的方法制备的杂交瘤产生：（1）用原核细胞来源的人LOX-1细胞外结构域免疫非人动物; （2）从动物收集产生抗体的细胞; （3）将抗体产生细胞与骨髓瘤细胞融合; （4）从上述步骤获得的融合细胞中选择产生与人LOX-1细胞外结构域反应的单克隆抗体的杂交瘤; 和（5）选择产生与来自所选杂交瘤的真核细胞来源的人LOX-1细胞外结构域反应的单克隆抗体的杂交瘤。

公开(公告)号：US20110045504A1

公开(公告)日：2011-02-24

申请号：US12086869

申请日：2006-12-21

申请人： Noriaki Kume ,  Goro Kominami

发明人： Noriaki Kume ,  Goro Kominami

IPC分类号： G01N33/566 ,  G01N33/68

CPC分类号： G01N33/566 ,  G01N33/92 ,  G01N2800/324

摘要： Problem is to provide a diagnostic marker for prognostic prediction of acute coronary syndrome. Means to solve the Problem is to make it possible to predict the risk of recurrence of acute coronary syndrome by measuring the concentration of soluble LOX-1 in the blood.

摘要翻译： 问题是为急性冠脉综合征的预后预测提供诊断标志。 解决问题的手段是通过测量血液中可溶性LOX-1的浓度来预测急性冠状动脉综合征复发的风险。

公开(公告)号：US20090203039A1

公开(公告)日：2009-08-13

申请号：US12375210

申请日：2007-07-27

申请人： Goro Kominami ,  Masahiro Nakamura ,  Noriaki Kume ,  Hideki Ohta

发明人： Goro Kominami ,  Masahiro Nakamura ,  Noriaki Kume ,  Hideki Ohta

IPC分类号： G01N33/53 ,  C07K16/00 ,  C12N5/06

CPC分类号： C07K16/28 ,  C07K2317/92

摘要： It is intended to provide a monoclonal antibody that specifically recognizes human soluble LOX-1, particularly a monoclonal antibody with a dissociation constant (Kd) for human soluble LOX-1 of 1×10−9 (M) or less. The antibody can be produced from a hybridoma prepared by a method including the following steps of: (1) immunizing a non-human animal with a prokaryotic cell-derived human LOX-1 extracellular domain; (2) collecting antibody-producing cells from the animal; (3) fusing the antibody-producing cells with myeloma cells; (4) selecting hybridomas that produce a monoclonal antibody reacting with the human LOX-1 extracellular domain from the fused cells obtained in the above step; and (5) selecting a hybridoma that produces a monoclonal antibody reacting with a eukaryotic cell-derived human LOX-1 extracellular domain from the selected hybridomas.

摘要翻译： 旨在提供特异性识别人可溶性LOX-1的单克隆抗体，特别是具有1x10-9（M）以下的人可溶性LOX-1的解离常数（Kd）的单克隆抗体。 抗体可以由通过包括以下步骤的方法制备的杂交瘤产生：（1）用原核细胞来源的人LOX-1细胞外结构域免疫非人动物; （2）从动物收集产生抗体的细胞; （3）将抗体产生细胞与骨髓瘤细胞融合; （4）从上述步骤获得的融合细胞中选择产生与人LOX-1细胞外结构域反应的单克隆抗体的杂交瘤; 和（5）选择产生与来自所选杂交瘤的真核细胞来源的人LOX-1细胞外结构域反应的单克隆抗体的杂交瘤。

公开(公告)号：US4272827A

公开(公告)日：1981-06-09

申请号：US21011

申请日：1979-03-16

申请人： Norio Inui ,  Noriaki Kume ,  Tetsuro Okamoto

发明人： Norio Inui ,  Noriaki Kume ,  Tetsuro Okamoto

IPC分类号： G06F7/52 ,  G06F7/527 ,  G06F7/535

CPC分类号： G06F7/535 ,  G06F2207/5355

摘要： A division processing system performs 2N-bit precision division processing by effectively using division processing circuitry with N-bit precision. The system performs the division with 2N-bit precision as follows: ##EQU1## (n=N: the number of digit positions in selected binary numbers A, B, C and D). The above expression is approximated to the form of Q.sub.1 +Q.sub.2 .times.2-n (Q.sub.1, Q.sub.2 : binary numbers). The binary numbers Q.sub.1 and Q.sub.2 are respectively operated on by the division processing circuitry with N-bit precision. By effective control, the error caused during the division processing of Q.sub.1 is used as a part of the data for performing the division processing of Q.sub.2, thus effectively transferring any error evolving during the processing of Q.sub.1 to Q.sub.2. The function is performed in a system having only four registers, each of N-bit capacity (precision), and an operation register, multiplication circuitry, division circuitry, and a shift circuit, affording proper control of data transfer between the registers.

摘要翻译： 分割处理系统通过有效地使用N位精度的分割处理电路来执行2N位精度分割处理。 系统以2N位精度进行除法，如下所示：（n = N：选定二进制数A，B，C和D中的位数）。 上述表达式近似为Q1 + Q2x2-n（Q1，Q2：二进制数）的形式。 二进制数Q1和Q2分别由具有N位精度的分割处理电路操作。 通过有效控制，在Q1的分割处理期间引起的误差被用作执行Q2的分割处理的数据的一部分，从而有效地将在Q1处理期间发生的任何错误转移到Q2。 该功能在仅具有四个寄存器，N位容量（精度）和操作寄存器，乘法电路，分频电路和移位电路的系统中执行，从而提供对寄存器之间的数据传输的适当控制。