公开(公告)号：US20200179880A1

公开(公告)日：2020-06-11

申请号：US16788733

申请日：2020-02-12

Applicant: Oxford Nanopore Inc.

Inventor： Ping Xie ,  Ken Healy ,  Justin Millis

IPC: B01D67/00 ,  C25F3/14 ,  C12Q1/6869 ,  B01D69/12 ,  G01N33/487 ,  C25F7/00 ,  B01D69/02 ,  C25F3/02 ,  B01D69/06

Abstract: Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown are provided. In one disclosed arrangement a plurality of apertures are formed. The membrane comprises a first surface area portion on one side of the membrane and a second surface area portion on the other side of the membrane. Each of a plurality of target regions comprises a recess or a fluidic passage opening out into the first or second surface area portion. The method comprises contacting all of the first surface area portion of the membrane with a first bath comprising ionic solution and all of the second surface area portion with a second bath comprising ionic solution. A voltage is applied across the membrane via first and second electrodes in respective contact with the first and second baths comprising ionic solutions to form an aperture at each of a plurality of the target regions in the membrane.

公开(公告)号：US10596523B2

公开(公告)日：2020-03-24

申请号：US15575798

申请日：2016-05-20

Applicant: Oxford Nanopore Inc.

Inventor： Ping Xie ,  Ken Healy ,  Justin Millis

IPC: B01D67/00 ,  B01D69/02 ,  C25F7/00 ,  G01N33/487 ,  B01D69/06 ,  C25F3/02 ,  B01D69/12 ,  C12Q1/6869 ,  C25F3/14 ,  B82Y40/00

Abstract: Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown are provided. In one disclosed arrangement a plurality of apertures are formed. The membrane comprises a first surface area portion on one side of the membrane and a second surface area portion on the other side of the membrane. Each of a plurality of target regions comprises a recess or a fluidic passage opening out into the first or second surface area portion. The method comprises contacting all of the first surface area portion of the membrane with a first bath comprising ionic solution and all of the second surface area portion with a second bath comprising ionic solution. A voltage is applied across the membrane via first and second electrodes in respective contact with the first and second baths comprising ionic solutions to form an aperture at each of a plurality of the target regions in the membrane.

公开(公告)号：US20180141007A1

公开(公告)日：2018-05-24

申请号：US15575798

申请日：2016-05-20

Applicant: Oxford Nanopore Inc.

Inventor： Ping Xie ,  Ken Healy ,  Justin Millis

IPC: B01D67/00 ,  B01D69/12 ,  B01D69/02 ,  C25F3/14 ,  C25F7/00 ,  G01N33/487 ,  C12Q1/6869

CPC classification number: B01D67/0062 ,  B01D69/02 ,  B01D69/06 ,  B01D69/12 ,  B01D2323/42 ,  B01D2325/021 ,  B01D2325/06 ,  B82Y40/00 ,  C12Q1/6869 ,  C25F3/02 ,  C25F3/14 ,  C25F7/00 ,  G01N33/48721

Abstract: Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown are provided. In one disclosed arrangement a plurality of apertures are formed. The membrane comprises a first surface area portion on one side of the membrane and a second surface area portion on the other side of the membrane. Each of a plurality of target regions comprises a recess or a fluidic passage opening out into the first or second surface area portion. The method comprises contacting all of the first surface area portion of the membrane with a first bath comprising ionic solution and all of the second surface area portion with a second bath comprising ionic solution. A voltage is applied across the membrane via first and second electrodes in respective contact with the first and second baths comprising ionic solutions to form an aperture at each of a plurality of the target regions in the membrane.