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    Isolation of nucleic acids using a polycationic polymer as precipitation agent
    1.
    发明申请
    Isolation of nucleic acids using a polycationic polymer as precipitation agent 审中-公开
    使用聚阳离子聚合物作为沉淀剂分离核酸

    公开(公告)号：US20050222404A1

    公开(公告)日：2005-10-06

    申请号：US10517227

    申请日：2003-06-26

    申请人： Igor Galaev ,  Per-Erik Gustavsson ,  Vladimir Izumrudov ,  Per-Olof Larsson ,  Per-Olof Wahlund

    发明人： Igor Galaev ,  Per-Erik Gustavsson ,  Vladimir Izumrudov ,  Per-Olof Larsson ,  Per-Olof Wahlund

    IPC分类号： C12N15/09 ,  C07H1/06 ,  C07H1/08 ,  C12N15/10 ,  C07H21/04

    CPC分类号： C07H1/06 ,  C07H1/08 ,  C12N15/1003

    摘要： The present invention relates to a method of isolating a desired nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+]/[−] between polycationic precipitating agent and nucleic acid is ≧0.5, preferably ≧0.9 and most preferably ≧1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the nucleic acid/polycation complex.

    摘要翻译： 本发明涉及从生物溶液中分离所需核酸的方法，该方法包括通过向溶液中加入聚阳离子沉淀剂并使其与所述核酸形成复合物来选择性沉淀所需核酸，其中 沉淀剂是包含季铵基的高度带电的线性聚合物。 聚阳离子沉淀剂的添加量优选在沉淀过程中阳离子沉淀剂与核酸之间的电荷比[+] / [ - ]优选≥0.5，优选> = 0.9，最优选≥1; 存在确保核酸/聚阳离子络合物的定量特异性沉淀的盐浓度。