-
1.
公开(公告)号:US20110171656A1
公开(公告)日:2011-07-14
申请号:US13063598
申请日:2009-09-16
CPC分类号: C12Q1/6806 , C12Q2545/101 , C12Q2523/308
摘要: The present invention relates to a method for normalizing the contents of biomolecules in a sample, comprising the following steps: a) preparing a reaction vessel with a vessel surface that is functionalized at least in sections—preferably on the inside of the vessel—in such a way that the surface can reversibly bind biomolecules under high salt conditions, b) executing at least one sample preparation step, c) binding biomolecules from the prepared sample to the vessel surface (“binding and normalizing step”) under high salt conditions, d) optionally washing (“washing step”), and e) executing at least one subsequent reaction. The application also relates to a reaction vessel as is used in the above method.
摘要翻译: 本发明涉及一种用于使样品中生物分子的内容物标准化的方法,包括以下步骤:a)制备具有至少在部分 - 优选在容器内部官能化的容器表面的反应容器 - 在这样的 表面可以在高盐条件下可逆地结合生物分子的方式,b)执行至少一个样品制备步骤,c)在高盐条件下将生物分子从制备的样品结合到容器表面(“结合和归一化步骤”),d )任选地洗涤(“洗涤步骤”),和e)执行至少一个后续反应。 本申请还涉及如上述方法中使用的反应容器。