公开(公告)号：US07087808B2

公开(公告)日：2006-08-08

申请号：US10790720

申请日：2004-03-03

Applicant: Shinn Chih Wu ,  Teng Kuei Cheng ,  Chuan Mu Chen ,  Shau Ping Lin ,  Chon Ho Yen ,  Ping Cheng Yang

Inventor： Shinn Chih Wu ,  Teng Kuei Cheng ,  Chuan Mu Chen ,  Shau Ping Lin ,  Chon Ho Yen ,  Ping Cheng Yang

IPC: C12P21/00 ,  A01K67/00 ,  A01K67/027 ,  C12N15/00 ,  C07H21/04

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/01

Abstract: The present invention discloses a method for expressing multiple recombinant proteins in transgenic non-human mammalian milk, characterized in which human clotting factor IX gene and porcine lactoferrin gene are transferred into the mammal by gene injection and embryonic implantation to obtain expression in the milk of transgenic mammal and its filial generation. The method of this invention can maintain the stable expression of multiple recombinant proteins in the transgenic mammal during lactation and stable expression amount proximate to that of the first generation in the offsprings of the transgenic non-human mammal.

Abstract translation: 本发明公开了一种在转基因非人哺乳动物乳中表达多种重组蛋白的方法，其特征在于通过基因注射和胚胎移植将人凝血因子IX基因和猪乳铁蛋白基因转移到哺乳动物中，以获得转基因乳中的表达 哺乳动物及其子代。 本发明的方法可以在哺乳期间保持转基因哺乳动物中多种重组蛋白的稳定表达，并且在转基因非人哺乳动物的后代中可以保持接近于第一代的稳定表达量。

公开(公告)号：US20070016969A1

公开(公告)日：2007-01-18

申请号：US10727145

申请日：2003-12-03

Applicant: Ching-Fu Tu ,  Chi-Kai Yang ,  Chich-Sheng Lin ,  Chon-Ho Yen ,  I-Chung Chen ,  Shinn-Chih Wu ,  Yu-Ling Sun ,  Ming-Shing Liu ,  Ping-Cheng Yang

Inventor： Ching-Fu Tu ,  Chi-Kai Yang ,  Chich-Sheng Lin ,  Chon-Ho Yen ,  I-Chung Chen ,  Shinn-Chih Wu ,  Yu-Ling Sun ,  Ming-Shing Liu ,  Ping-Cheng Yang

IPC: A01K67/027 ,  C07H21/04 ,  C12P21/06 ,  C07K14/815

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/01 ,  C07K14/4732 ,  C07K14/815

Abstract: The present invention relates to a nucleic acid construct encoding hirudin and a transgenic mammal whose genome comprises the nucleic acid construct as well as a process for producing hirudin from the transgenic mammal.

Abstract translation: 本发明涉及编码水蛭素和转基因哺乳动物的核酸构建体，其基因组包含核酸构建体以及从转基因哺乳动物生产水蛭素的方法。

公开(公告)号：US20050114911A1

公开(公告)日：2005-05-26

申请号：US10790720

申请日：2004-03-03

Applicant: Shinn-Chih Wu ,  Teng-Kuei Cheng ,  Chuan-Mu Chen ,  Shau-Ping Lin ,  Chon-Ho Yen ,  Ping-Cheng Yang

Inventor： Shinn-Chih Wu ,  Teng-Kuei Cheng ,  Chuan-Mu Chen ,  Shau-Ping Lin ,  Chon-Ho Yen ,  Ping-Cheng Yang

IPC: A01K67/027

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/01

Abstract: The present invention discloses a method for expressing multiple recombinant proteins in transgenic non-human mammalian milk, characterized in which human clotting factor IX gene and porcine lactoferrin gene are transferred into the mammal by gene injection and embryonic implantation to obtain expression in the milk of transgenic mammal and its filial generation. The method of this invention can maintain the stable expression of multiple recombinant proteins in the transgenic mammal during lactation and stable expression amount proximate to that of the first generation in the offsprings of the transgenic non-human mammal.

Abstract translation: 本发明公开了一种在转基因非人哺乳动物乳中表达多种重组蛋白的方法，其特征在于通过基因注射和胚胎移植将人凝血因子IX基因和猪乳铁蛋白基因转移到哺乳动物中，以获得转基因乳中的表达 哺乳动物及其子代。 本发明的方法可以在哺乳期间保持转基因哺乳动物中多种重组蛋白的稳定表达，并且在转基因非人哺乳动物的后代中可以保持接近于第一代的稳定表达量。