公开(公告)号：US20090254065A1

公开(公告)日：2009-10-08

申请号：US11988924

申请日：2005-09-23

申请人： Chang-Kwon Ko ,  Eun-Young Lee ,  Jeong-Yong Choi ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Pyoung-Min Kim

发明人： Chang-Kwon Ko ,  Eun-Young Lee ,  Jeong-Yong Choi ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Pyoung-Min Kim

IPC分类号： A61M37/00

CPC分类号： A61K38/363 ,  A61K38/39 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/50 ,  A61L2430/06 ,  A61K2300/00

摘要： A method of transplanting an injectable chondrocyte is developed for autologous chondrocyte transplantation, comprising mixing matrices including fibrin, hyaluronic acid and collagen, which are the main ingredients of animal cartilage, and injecting the resulting mixture into a damaged cartilage region. One 1 ml syringe is prepared by mixing 1 ml of a chondrocyte culture with white or light-yellow lyophilized powder (fibrinogen). A second 1 ml syringe is prepared by mixing 1 ml of chondrocyte culture with white lyophilized powder (thrombin), adding 0.1 cc of that mixture to the entire contents of two vials containing chondrocyte suspension, and mixing well. The two syringes are then mounted on a syringe stand, and a mixing tip is mounted to their tips. The contents are mixed in the mixing tip while injecting the resulting mixture into a damaged cartilage region of an animal.

摘要翻译： 开发了用于自体软骨细胞移植的移植注射软骨细胞的方法，包括混合作为动物软骨的主要成分的纤维蛋白，透明质酸和胶原的基质，并将所得混合物注入损伤的软骨区域。 通过将1ml软骨细胞培养物与白色或浅黄色冻干粉末（纤维蛋白原）混合来制备1ml注射器。 通过将1ml软骨细胞培养物与白色冻干粉末（凝血酶）混合，将0.1cc该混合物加入到含有软骨细胞悬液的两个小瓶的全部内容物中并混合，制备第二个1ml注射器。 然后将两个注射器安装在注射器支架上，并将混合头安装到其尖端。 将内容物混合在混合尖端中，同时将所得混合物注入动物的损伤的软骨区域。