公开(公告)号：US20090325275A1

公开(公告)日：2009-12-31

申请号：US12309868

申请日：2007-02-15

申请人： Jeong-Yong Choi ,  Dong-Sam Suh ,  Chang-Kwon Ko ,  Seung-Ju Ryu ,  Hyang-Soon Chang ,  Jin-Wook Chung ,  Cheong-Ho Chang

发明人： Jeong-Yong Choi ,  Dong-Sam Suh ,  Chang-Kwon Ko ,  Seung-Ju Ryu ,  Hyang-Soon Chang ,  Jin-Wook Chung ,  Cheong-Ho Chang

IPC分类号： C12M1/18

CPC分类号： C12M37/00 ,  B01L1/04 ,  F24F3/1607

摘要： Cell manipulation and cultivation equipment for the production of cell therapy products has developed for preventing contamination of cells and improving the utility of a space. The equipment includes a room for the production and testing of cell therapy products, the room having an inner space enclosed in all sides and an outer entrance/exit door provided at any one side thereof, an L-shaped partition installed in a position of the room for the production and testing of cell therapy products, the partition having an inner entrance/exit door provided at any one side thereof, and a clean bench device placed inside the partition, the clean bench device including first, second, and third clean benches for preventing contamination of cells and increasing the utility of a space to the maximum extent. The operating of the clean room and clean bench has advantages to prevent contamination and improving the quality and reliability of cell therapy products.

摘要翻译： 用于生产细胞治疗产品的细胞操作和培养设备已经开发用于防止细胞污染和改善空间的效用。 该设备包括用于生产和测试细胞治疗产品的房间，房间具有封闭在其内侧的内部空间和设置在其任一侧的外部入口/出口门，L形分隔件安装在 用于生产和测试细胞治疗产品的空间，所述隔板具有设置在其任一侧的内入口/出口门，以及放置在隔板内的洁净工作台装置，所述清洁台装置包括第一，第二和第三清洁台 用于防止细胞的污染和最大程度地增加空间的效用。 无尘室和洁净台的运行具有防止污染，提高细胞治疗产品质量和可靠性的优点。

公开(公告)号：US20090254065A1

公开(公告)日：2009-10-08

申请号：US11988924

申请日：2005-09-23

申请人： Chang-Kwon Ko ,  Eun-Young Lee ,  Jeong-Yong Choi ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Pyoung-Min Kim

发明人： Chang-Kwon Ko ,  Eun-Young Lee ,  Jeong-Yong Choi ,  Jae-Deog Jang ,  Cheong-Ho Chang ,  Pyoung-Min Kim

IPC分类号： A61M37/00

CPC分类号： A61K38/363 ,  A61K38/39 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/50 ,  A61L2430/06 ,  A61K2300/00

摘要： A method of transplanting an injectable chondrocyte is developed for autologous chondrocyte transplantation, comprising mixing matrices including fibrin, hyaluronic acid and collagen, which are the main ingredients of animal cartilage, and injecting the resulting mixture into a damaged cartilage region. One 1 ml syringe is prepared by mixing 1 ml of a chondrocyte culture with white or light-yellow lyophilized powder (fibrinogen). A second 1 ml syringe is prepared by mixing 1 ml of chondrocyte culture with white lyophilized powder (thrombin), adding 0.1 cc of that mixture to the entire contents of two vials containing chondrocyte suspension, and mixing well. The two syringes are then mounted on a syringe stand, and a mixing tip is mounted to their tips. The contents are mixed in the mixing tip while injecting the resulting mixture into a damaged cartilage region of an animal.

摘要翻译： 开发了用于自体软骨细胞移植的移植注射软骨细胞的方法，包括混合作为动物软骨的主要成分的纤维蛋白，透明质酸和胶原的基质，并将所得混合物注入损伤的软骨区域。 通过将1ml软骨细胞培养物与白色或浅黄色冻干粉末（纤维蛋白原）混合来制备1ml注射器。 通过将1ml软骨细胞培养物与白色冻干粉末（凝血酶）混合，将0.1cc该混合物加入到含有软骨细胞悬液的两个小瓶的全部内容物中并混合，制备第二个1ml注射器。 然后将两个注射器安装在注射器支架上，并将混合头安装到其尖端。 将内容物混合在混合尖端中，同时将所得混合物注入动物的损伤的软骨区域。

公开(公告)号：US20070087032A1

公开(公告)日：2007-04-19

申请号：US10578719

申请日：2004-12-07

申请人： Cheong-Ho Chang ,  Chang-Kwon Ko ,  Jae-Deog Jang ,  Eun-Young Lee ,  Jeong-Yong Choi

发明人： Cheong-Ho Chang ,  Chang-Kwon Ko ,  Jae-Deog Jang ,  Eun-Young Lee ,  Jeong-Yong Choi

IPC分类号： A61F2/00

CPC分类号： A61K35/32 ,  A61F2/30756 ,  A61K35/36 ,  A61K35/39 ,  A61L27/225 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61K2300/00

摘要： A cartilage therapeutic composition is developed for clinical transplantation into articulatio genu (knee joints) or ankle joints. It has clinical significance for symptomatic cartilage defects of the femoral condyle (medial, lateral, or trochlear) and bone cartilage defects of the talus (anklebone) in human or animal hosts, The cartilage therapeutic composition comprises a mixture of components of chondrocytes isolated and expanded or differentiated from a host such as a human or animal, and thrombin and a fibrinogen matrix containing fibrinogen. An application of the cartilage therapeutic composition is that a mixture of thrombin, chondrocyte components and a fibrinogen matrix is injected into a cartilage defect region followed by solidification therein. It provides rapid healing and effective regeneration of cartilage without surgical operation. It has the merits of safety and simplicity by allowing the use of an arthroscope for transplantation.

摘要翻译： 软骨治疗组合物被开发用于临床移植到关节（膝关节）或踝关节。 对于人或动物宿主中的距骨（踝骨）的股骨髁（内侧，外侧或滑车）和骨软骨缺损的症状性软骨缺损具有临床意义。软骨治疗组合物包含分离和扩大的软骨细胞成分的混合物 或与宿主如人或动物分化，以及含有纤维蛋白原的凝血酶和纤维蛋白原基质。 软骨治疗组合物的应用是将凝血酶，软骨细胞成分和纤维蛋白原基质的混合物注射到软骨缺损区域中，随后在其中固化。 它可以快速愈合，有效地再生软骨，无需外科手术。 通过允许使用关节镜进行移植，具有安全和简单的优点。