Cloned genes encoding the D.sub.1 dopamine receptor
    1.
    发明授权
    Cloned genes encoding the D.sub.1 dopamine receptor 失效
    编码D1多巴胺受体的克隆基因

    公开(公告)号:US5389543A

    公开(公告)日:1995-02-14

    申请号:US908654

    申请日:1992-07-02

    摘要: Cloned genes which code for the D.sub.1 dopamine receptor are disclosed. The receptors coded for by these clones bind dopamine ligands with the proper pharmacological profile and, when expressed in the cell membrane of a suitable host and so bound, stimulate adenylyl cyclase. Also disclosed are vectors comprising a cloned gene encoding a D.sub.1 -dopamine receptor, cells transformed with such vectors, and oligonucleotide probes capable of selectively hybridizing to DNA comprising a portion of a gene coding for a D.sub.1 -dopamine receptor. The cloned genes are useful for making proteins and cell membrane preparations which can be used to screen compounds for D.sub.1 -dopamine receptor binding activity, are useful in molecular biology, and are useful as diagnostic probes.

    摘要翻译: 公开了编码D1多巴胺受体的克隆基因。 由这些克隆编码的受体结合具有适当药理学特征的多巴胺配体,并且当在合适的宿主的细胞膜中表达并如此结合时,刺激腺苷酸环化酶。 还公开了包含编码D1-多巴胺受体的克隆基因,用这些载体转化的细胞和能够与包含编码D1-多巴胺受体的基因的一部分的DNA选择性杂交的寡核苷酸探针的载体。 克隆的基因可用于制备可用于筛选化合物的多巴胺受体结合活性的蛋白质和细胞膜制剂,可用于分子生物学,并且可用作诊断探针。

    Aqueous solution containing D.sub.1 dopamine receptor
    2.
    发明授权
    Aqueous solution containing D.sub.1 dopamine receptor 失效
    含有D1多巴胺受体的水溶液

    公开(公告)号:US5547845A

    公开(公告)日:1996-08-20

    申请号:US196523

    申请日:1994-02-15

    摘要: Cloned genes which code for the D.sub.1 dopamine receptor are disclosed. The receptors coded for by these clones bind dopamine ligands with the proper pharmacological profile and, when expressed in the cell membrane of a suitable host and so bound, stimulate adenylyl cyclase. Also disclosed are vectors comprising a cloned gene encoding a D.sub.1 -dopamine receptor, cells transformed with such vectors, and oligonucleotide probes capable of selectively hybridizing to DNA comprising a portion of a gene coding for a D.sub.1 -dopamine receptor. The cloned genes are useful for making proteins and cell membrane preparations which can be used to screen compounds for D.sub.1 -dopamine receptor binding activity, are useful in molecular biology, and are useful as diagnostic probes.

    摘要翻译: 公开了编码D1多巴胺受体的克隆基因。 由这些克隆编码的受体结合具有适当药理学特征的多巴胺配体,并且当在合适的宿主的细胞膜中表达并如此结合时,刺激腺苷酸环化酶。 还公开了包含编码D1-多巴胺受体的克隆基因,用这些载体转化的细胞和能够与包含编码D1-多巴胺受体的基因的一部分的DNA选择性杂交的寡核苷酸探针的载体。 克隆的基因可用于制备可用于筛选化合物的多巴胺受体结合活性的蛋白质和细胞膜制剂,可用于分子生物学,并且可用作诊断探针。