公开(公告)号：US5441745A

公开(公告)日：1995-08-15

申请号：US663550

申请日：1984-10-22

申请人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

发明人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

IPC分类号： A61K9/127 ,  A61K9/133 ,  A61K51/12

CPC分类号： A61K9/1271 ,  A61K51/1234 ,  A61K2121/00 ,  A61K2123/00 ,  Y10S436/829

摘要： A method is provided for delivering micellular particles containing chemotherapeutic agents and a marker to tumors within a body for the diagnosis and treatment of such tumors. The micellular particles are small, less than 2000 .ANG. and incorporate pure, neutral phospholipid molecules in their external surface. Enhanced delivery of the micellular particles containing marker and chemotherapeutic agents may be achieved by introducing an initial group of positively charged micellular particles to block the reticuloendothelial cells present in the body.

摘要翻译： 提供了一种用于将包含化学治疗剂的微粒颗粒和用于诊断和治疗这样的肿瘤的体内的肿瘤的标记递送到该体内的方法。 胶束颗粒小，小于2000 ANGSTROM，并在其外表面掺入纯中性磷脂分子。 通过引入带正电荷的微胶粒的初始组来阻止存在于体内的网状内皮细胞，可以实现含有标记物和化学治疗剂的胶束颗粒的增强递送。

公开(公告)号：US4493899A

公开(公告)日：1985-01-15

申请号：US312159

申请日：1981-10-16

申请人： Lloyd H. Smith ,  Raymond L. Teplitz

发明人： Lloyd H. Smith ,  Raymond L. Teplitz

IPC分类号： C12Q1/68 ,  G01N33/564 ,  G01N33/54 ,  G01N33/56 ,  C12Q1/44

CPC分类号： G01N33/564 ,  C12Q1/68 ,  G01N2800/104 ,  Y10S436/804 ,  Y10S436/811 ,  Y10S436/823 ,  Y10S436/824 ,  Y10S436/825

摘要： A method is provided for testing for particular antibodies in the serum of a patient. The antibodies may be those of systemic lupus erythematosus and may constitute IgG and IgM immunoglobulins. The IgG and IgM immunoglobulin may be individually labeled radioactively.An antigen (such as DNA) may be attached to a support such as sepharose. The attachment may be facilitated as by irradiation with ultraviolet light. The DNA may be single stranded or double stranded. When double-stranded DNA is used, single-stranded portions in the double strands may be removed as by a suitable enzyme.The particular antibodies may be attached to the antigen such as the supported DNA. An assay may then be provided to determine the attachment of the particular antibodies to the supported DNA. When the particular antibodies constitute immunoglobins of systemic lupus erythematosus, the assay may actually provide a determination of the amounts of the IgG and IgM immunoglobulins individually attached to the single-stranded and double-stranded DNA.

摘要翻译： 提供了一种用于测试患者血清中特定抗体的方法。 抗体可以是系统性红斑狼疮的抗体，并且可以构成IgG和IgM免疫球蛋白。 IgG和IgM免疫球蛋白可以放射性地单独标记。 可以将抗原（例如DNA）附着到诸如sepharose的载体上。 可以通过用紫外线照射来促进附着。 DNA可以是单链或双链的。 当使用双链DNA时，可以通过合适的酶除去双链中的单链部分。 特定的抗体可以连接到抗原上，例如被支持的DNA。 然后可以提供测定以确定特定抗体与所支持的DNA的连接。 当特定抗体构成系统性红斑狼疮的免疫球蛋白时，该测定实际上可以提供单独连接到单链和双链DNA的IgG和IgM免疫球蛋白的量的测定。

公开(公告)号：US06274115B1

公开(公告)日：2001-08-14

申请号：US08483494

申请日：1995-06-07

申请人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

发明人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

IPC分类号： A61K5100

CPC分类号： A61K9/1271 ,  A61K51/1234 ,  A61K2121/00 ,  A61K2123/00 ,  Y10S436/829

摘要： Micellular particles such as small unilamellar vesicles of less than 2000 A° loaded with 111In are administered to BALB/c mice in which EMT6 tumors had been induced. Whole body scintographs of the mice to which either neutral or positively or negatively charged vesicles had been administered show a substantial quantity of the vesicle entrapped 111In localized in the tumor. Blocking of macrophages in the liver and spleen by first administering unlabeled, aminomannose substituted vesicles before administration of the labeled vesicles increases uptake of the 111In labeled vesicles in the tumor.

摘要翻译： 将具有小于2000A的小单层囊泡的胶束颗粒（装载有111In）施用于已经诱导EMT6肿瘤的BALB / c小鼠。 已经施用中性或正性或带负电荷的小泡的小鼠的全身阴影图显示了定量存在于肿瘤中的大量囊泡111I。 在施用标记的囊泡之前首先施用未标记的氨基甘露糖糖溶液取代的囊泡，在肝脏和脾脏中阻断巨噬细胞增加肿瘤中111I标记的囊泡的摄取

公开(公告)号：US5435989A

公开(公告)日：1995-07-25

申请号：US663503

申请日：1984-10-22

申请人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

发明人： Cary A. Presant ,  Richard T. Proffitt ,  Raymond L. Teplitz ,  Lawrence E. Williams ,  George W. Tin

IPC分类号： A61K9/127 ,  A61K9/133 ,  A61K51/12

CPC分类号： A61K9/1271 ,  A61K51/1234 ,  A61K2121/00 ,  A61K2123/00 ,  Y10S436/829

摘要： Micellular particles such as small unilamellar vesicles of less than 2000 .ANG. loaded with .sup.111 In are administered to BALB/c mice in which EMT6 tumors had been induced. Whole body scintographs of the mice to which either neutral or positively or negatively charged vesicles had been administered show a substantial quantity of the vesicle entrapped .sup.111 In localized in the tumor. Blocking of macrophages in the liver and spleen by first administering unlabeled, aminomannose substituted vesicles before administration of the labeled vesicles increases uptake of the .sup.111 In labeled vesicles in the tumor.

摘要翻译： 将具有小于2000个ANGSTROM的小单层囊泡的胶束颗粒施用于已经诱导EMT6肿瘤的BALB / c小鼠。 已经施用中性或正性或带负电荷的小泡的小鼠的全身阴影图显示了定量存在于肿瘤中的大量囊泡111I。 在施用标记的囊泡之前首先施用未标记的氨基甘露糖取代的囊泡，在肝脏和脾脏中阻断巨噬细胞增加了肿瘤中111I标记的囊泡的摄取。