公开(公告)号：US08873707B2

公开(公告)日：2014-10-28

申请号：US13567613

申请日：2012-08-06

申请人： Eva R. Birnbaum ,  Benjamin P. Warner ,  Sharon M. Baldwin ,  Jennifer A. Berger ,  Rebecca L. E. Miller

发明人： Eva R. Birnbaum ,  Benjamin P. Warner ,  Sharon M. Baldwin ,  Jennifer A. Berger ,  Rebecca L. E. Miller

IPC分类号： G01N1/28

CPC分类号： G01N23/2202 ,  G01N23/2204 ,  G01N23/223 ,  G01N2223/074 ,  G01N2223/076 ,  G01N2223/309 ,  G01N2223/64 ,  G03B42/02

摘要： An apparatus for preparing samples for measurement by x-ray fluorescence spectrometry. A plate has one or more holes passing through the plate. The holes are covered by a film on one side of the plate. The holes are less than 500 micrometers across in one dimension where the film covers the holes. The film is translucent to x-rays.

摘要翻译： 通过X射线荧光光谱法制备用于测量的样品的装置。 板有一个或多个孔穿过板。 孔被板的一侧的薄膜覆盖。 在膜覆盖孔的一个维度上，孔小于500微米。 这部电影对于x射线是半透明的。

公开(公告)号：US08431357B2

公开(公告)日：2013-04-30

申请号：US12496532

申请日：2009-07-01

申请人： Eva R. Birnbaum ,  Rebecca L. E. Miller ,  Lori J. Peterson ,  Jennifer A. Berger ,  Benjamin P. Warner

发明人： Eva R. Birnbaum ,  Rebecca L. E. Miller ,  Lori J. Peterson ,  Jennifer A. Berger ,  Benjamin P. Warner

IPC分类号： C12Q1/02 ,  G01N21/76 ,  G01N23/223

CPC分类号： G01N33/6872 ,  G01N23/2076 ,  G01N23/223 ,  G01N33/502 ,  G01N2223/076

摘要： The present invention includes a method and apparatus for measuring the removal of analyte from a cell. One or more cells are provided, comprising at least 10 picograms of an analyte within a volume defined by the area of an x-ray excitation beam and a depth of a depth of five times the 1/e attenuation depth for at least one characteristic x-ray signal of the analyte as attenuated by water. At least a of portion of the analyte is removed from the cells; and the analyte is measured using x-ray fluorescence.

摘要翻译： 本发明包括用于测量从细胞中去除分析物的方法和装置。 提供了一个或多个细胞，其包括由X射线激发束的区域限定的体积内的至少10皮克分析物和对于至少一个特征x的1 / e衰减深度的深度的5倍 分析物的射线信号被水减弱。 从细胞中去除至少一部分分析物; 并使用x射线荧光测量分析物。

公开(公告)号：US08238515B2

公开(公告)日：2012-08-07

申请号：US12192762

申请日：2008-08-15

申请人： Eva R. Birnbaum ,  Benjamin P. Warner ,  Sharon M. Baldwin ,  Jennifer A. Berger ,  Rebecca L. E. Miller

发明人： Eva R. Birnbaum ,  Benjamin P. Warner ,  Sharon M. Baldwin ,  Jennifer A. Berger ,  Rebecca L. E. Miller

IPC分类号： G01N23/223

CPC分类号： G01N23/2202 ,  G01N23/2204 ,  G01N23/223 ,  G01N2223/074 ,  G01N2223/076 ,  G01N2223/309 ,  G01N2223/64 ,  G03B42/02

摘要： The present invention includes an apparatus for preparing samples for measurement by x-ray fluorescence spectrometry. The apparatus comprises a plate having one or more holes passing through the plate. The holes are covered by a film on one side of the plate. The holes are less than 500 micrometers across in one dimension where the film covers the holes. The film is translucent to x-rays. The present invention also includes an apparatus for preparing samples for measurement by x-ray fluorescence spectrometry. The apparatus comprises a plate having one or more holes passing through the plate. The holes are covered on one side of the plate by a detachable cover forming a water-tight seal against the plate. The cover is substantially free of the elements osmium, yttrium, iridium, phosphorus, zirconium, platinum, gold, niobium, mercury, thallium, molybdenum, sulfur, lead, bismuth, technetium, ruthenium, chlorine, rhodium, palladium, argon, silver, and thorium. The holes are less than about 500 micrometers across in one dimension where the cover covers the holes. The present invention also includes a method for preparing samples for measurement by x-ray fluorescence spectrometry. The method comprises providing a solution of with less than 10 micromolar solute and a volume of between about 2 microliters and about 2 milliliters. The solution is concentrated and analyzed using x-ray fluorescence spectrometry.

摘要翻译： 本发明包括通过X射线荧光光谱法制备用于测量的样品的装置。 该装置包括具有穿过板的一个或多个孔的板。 孔被板的一侧的薄膜覆盖。 在膜覆盖孔的一个维度上，孔小于500微米。 这部电影对于x射线是半透明的。 本发明还包括通过X射线荧光光谱法制备用于测量的样品的装置。 该装置包括具有穿过板的一个或多个孔的板。 孔通过可拆卸的盖子覆盖在板的一侧上，该盖子形成对板的防水密封。 该盖基本上不含锇，钇，铱，磷，锆，铂，金，铌，汞，铊，钼，硫，铅，铋，锝，钌，氯，铑，钯，氩， 和钍。 这些孔在一个尺寸上小于约500微米，其中盖覆盖孔。 本发明还包括通过X射线荧光光谱法制备用于测量的样品的方法。 该方法包括提供小于10微摩尔溶质的溶液和约2微升至约2毫升的体积。 使用x射线荧光光谱法浓缩溶液并进行分析。