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公开(公告)号:US08808991B2
公开(公告)日:2014-08-19
申请号:US10570249
申请日:2004-08-31
CPC分类号: C12Q1/6858 , C12Q1/6827 , C12Q1/6862 , C12Q2525/155 , C12Q2521/501 , C12Q2521/319 , C12Q2565/125 , C12Q2563/179 , C12Q2525/125 , C12Q2525/186 , C12Q2521/325
摘要: Method for the detection of a target sequence comprising ligating two probes when annealed adjacent to the target sequence, hybridization of a compound primer to the ligated probes and after elongation of the compound primer, amplifying the elongated compound primer from primers annealing to primer binding sites provided in the compound primer and one of the probes to produce detectably amplicons.
摘要翻译: 用于检测靶序列的方法,包括在与靶序列相邻退火时连接两个探针,化合物引物与连接的探针杂交,并且在化合物引物延伸之后,将引物退火引物延伸至引物结合位点 在复合引物和探针之一产生可检测的扩增子。