公开(公告)号：US07338778B2

公开(公告)日：2008-03-04

申请号：US10957802

申请日：2004-10-04

申请人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

发明人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

IPC分类号： C12Q1/04

CPC分类号： C12Q1/18 ,  C12Q1/04 ,  C12Q2304/44 ,  G06F19/00 ,  Y02A90/26

摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。

公开(公告)号：US06900030B2

公开(公告)日：2005-05-31

申请号：US10116777

申请日：2002-04-04

申请人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

发明人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

IPC分类号： C12Q1/04 ,  C12Q1/18

CPC分类号： C12Q1/18 ,  C12Q1/04 ,  C12Q2304/44 ,  G06F19/00 ,  Y02A90/26

摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。

公开(公告)号：US06395506B1

公开(公告)日：2002-05-28

申请号：US09342720

申请日：1999-06-29

申请人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

发明人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

IPC分类号： C12Q118

CPC分类号： C12Q1/18 ,  C12Q1/04 ,  C12Q2304/44 ,  G06F19/00 ,  Y02A90/26

摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。