公开(公告)号：US4816168A

公开(公告)日：1989-03-28

申请号：US923909

申请日：1986-10-28

申请人： Richard J. Carrol ,  Albert A. Fluderer ,  Ward C. Smith ,  Anthony R. Zine, Jr.

发明人： Richard J. Carrol ,  Albert A. Fluderer ,  Ward C. Smith ,  Anthony R. Zine, Jr.

IPC分类号： G01N33/50 ,  G01N33/48 ,  G01N33/49 ,  B01D21/26

CPC分类号： G01N33/491 ,  Y10T436/25375

摘要： This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.