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公开(公告)号：US4978502A
公开(公告)日：1990-12-18
申请号：US130616
申请日：1987-12-15
申请人： Charles M. Dole , Gary L. Webster , Ward C. Smith
发明人： Charles M. Dole , Gary L. Webster , Ward C. Smith
CPC分类号： B01L3/502 , B01L2400/0644 , G01N1/40 , G01N2001/2873 , G01N2001/288 , Y10S435/81 , Y10S436/808 , Y10S436/809
摘要： Liquid containers or receptacles are disclosed with cooperating means for rupturing the receptacles to release contained fluid to rain upon test specimens. Structure is disclosed for rupturing receptacles in response to relative motion occurring between receptacles and test specimen support members or in response to punch means formed integrally with a receptacle. A method of molding a liquid receptacle with an integrally formed punch means for rupturing the receptacle is also disclosed.
摘要翻译： 公开了液体容器或容器，其具有合作装置，用于破裂容器以将测试样品上的所含流体释放到雨中。 公开了用于响应于在容器和测试样本支撑构件之间发生的相对运动或响应于与容器一体形成的冲压装置而破裂插座的结构。 还公开了一种用整体形成的用于破裂容器的冲头装置来模制液体容器的方法。
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公开(公告)号：US4957638A
公开(公告)日：1990-09-18
申请号：US349600
申请日：1989-05-09
申请人： Ward C. Smith
发明人： Ward C. Smith
IPC分类号： G01N33/49
CPC分类号： G01N33/491 , Y10T436/25375
摘要： An assembly for separating lymphocytes and monocytes from granulocytes in a sample of unseparated whole blood. A water insoluble, thixotropic gel-like substance, which is chemically inert to blood constituents is provided in a container. A fluid capable of altering blood cell densities and diameters is positioned above the surface of the water-insoluble thixotropic gel-like substance. Means for preventing the absorption of water by the thixotropic gel-like substance from the fluid and/or the sample of unseparated whole blood prior to separation are provided to substantially eliminate the influence of water absorption on the cell separation performance characteristics of said thixotropic gel-like substance. A method for separating lymphocytes and monocytes from granulocytes which inhibits the apparent shift of the buoyant density of the granulocytes and which substantially eliminates the influence of water absorption on the cell separation performance characteristics of the thixotropic gel-like substance is also disclosed.
摘要翻译： 用于在未分离全血样品中分离淋巴细胞和单核细胞与粒细胞的组合体。 在容器中提供对血液成分具有化学惰性的水不溶性触变凝胶状物质。 能够改变血液细胞密度和直径的液体位于水不溶性触变凝胶状物质的表面上方。 提供了在分离前防止来自流体和/或未分离全血样品的触变凝胶状物质吸收水的手段，以基本上消除吸水对所述触变凝胶状物质的细胞分离性能特征的影响， 像物质。 还公开了一种从粒细胞分离淋巴细胞和单核细胞的方法，其抑制粒细胞的浮力密度的明显变化，并且基本上消除了吸水对触变凝胶状物质的细胞分离性能特征的影响。
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公开(公告)号：US4640785A
公开(公告)日：1987-02-03
申请号：US685801
申请日：1984-12-24
CPC分类号： G01N33/491 , Y10T436/25375
摘要： This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
摘要翻译： 本发明涉及用于抑制浮力密度的明显变化和/或恢复血液样品中粒细胞白细胞的浮力密度的任何损失的手段，从而确保淋巴细胞和单核细胞分离的质量 来自血液样品中的粒细胞。 本发明包括使血液样品与含有低分子量有机或无机离子物质的高渗流体和/或含有高分子量有机物质的等渗或高渗流体接触，所述高分子量或高渗流体可以含有在其结构中具有亲脂性取代基的有机分子， /或用于血细胞的培养基。 本发明的一个组成部分是利用比重在1.060-1.065g / cm 3之间的凝胶状物质的改进的血液分离管，以显着提高细胞分离的纯度，同时提供可接受的细胞产量。
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公开(公告)号：US4608110A
公开(公告)日：1986-08-26
申请号：US702809
申请日：1985-02-19
申请人： Ward C. Smith
发明人： Ward C. Smith
CPC分类号： B65H35/0026 , Y10T156/1052 , Y10T156/1062 , Y10T156/1348 , Y10T156/1365 , Y10T156/1795 , Y10T156/18
摘要： An apparatus is disclosed for dispensing discrete lengths of adhesive tape onto a substrate. A take-off reel is rotatably mounted in a housing having an opening through which tape is dispensed. A supply reel, a curved flat spring guide, a tape cutting blade and a dispenser mechanism are all located within the take-off reel. The guide reverses the direction of the tape from the supply reel and presses the adhesive side thereof against the inner surface of the take-off reel. A detent mechanism resists the rotation of the take-off reel after predetermined angular rotations thereof. When properly indexed, the take-off reel is rotated one detent position to properly align the tape with respect to the blade. The blade is actuated to cut from the tape a discrete label which can be dispensed.
摘要翻译： 公开了一种将离散长度的粘合带分配到基底上的装置。 取出卷轴可旋转地安装在具有分配带的开口的壳体中。 供带盘，弯曲的弹簧引导件，带切割刀片和分配机构都位于送出卷轴内。 引导件从供带盘反转带的方向，并将其粘合侧按压在取出卷轴的内表面上。 定位机构在其预定的旋转角度之后抵抗送出卷轴的旋转。 当适当地分度时，取出卷轴旋转一个制动位置以使带相对于叶片正确对准。 刀片被致动以从胶带切割可以分配的离散标签。
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公开(公告)号：US5494590A
公开(公告)日：1996-02-27
申请号：US897305
申请日：1992-06-11
申请人： Ward C. Smith , Richard J. Carroll
发明人： Ward C. Smith , Richard J. Carroll
CPC分类号： G01N33/5002 , G01N33/4905 , Y10T436/25375
摘要： An anticoagulant solution of sodium citrate for use in blood chemistry-related techniques and apparatus is disclosed. The anticoagulant solution should include an effective concentration of sodium citrate sufficient for preventing the coagulation of a sample of blood employed in the technique or added to the apparatus. The sodium citrate-based anticoagulant solution should have a pH ranging from above 6.0 to about 8.5 and a sodium citrate concentration preferably ranging from about 0.05M to about 0.2M.
摘要翻译： 公开了一种用于血液化学相关技术和装置中的柠檬酸钠抗凝血剂溶液。 抗凝血剂溶液应包括足够的有效浓度的柠檬酸钠，以防止该技术中使用的血液样品的凝结或添加到该装置中。 柠檬酸钠基抗凝血剂溶液的pH应在6.0至8.5之间，柠檬酸钠浓度优选为约0.05M至约0.2M。
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公开(公告)号：US4917801A
公开(公告)日：1990-04-17
申请号：US117396
申请日：1987-11-02
申请人： Albert A. Luderer , Ward C. Smith
发明人： Albert A. Luderer , Ward C. Smith
CPC分类号： G01N33/491 , B01D21/262 , B01D43/00 , B01D2221/10
摘要： This invention relates to an assembly for centrifugally separating lymphocytes and monocytes from the heavier phases in samples of human blood utilizing a liquid density gradient medium. The particular inventive subject matter concerns the use of novel partition means initially separating the liquid density gradient medium from the sample of blood and designs for such partition means.
摘要翻译： 本发明涉及一种利用液体密度梯度培养基从人体血液样本重离子中离心分离淋巴细胞和单核细胞的装置。 具体的发明主题涉及使用新颖的分隔装置，其最初将液体密度梯度介质从血液样品中分离出来并用于这种分隔装置。
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公开(公告)号：US4867887A
公开(公告)日：1989-09-19
申请号：US217947
申请日：1988-07-12
申请人： Ward C. Smith
发明人： Ward C. Smith
CPC分类号： G01N33/491 , B01D21/262 , B01L3/50215 , G01N33/5002 , B01D2221/10 , Y10T436/25375
摘要： A blood separation device includes a collection tube containing a first layer of a thixotropic gel-like substance and a second layer of a newtonian gel-like substance which is positioned below the first layer. The first layer of the thixotropic substance is positioned between the second layer of the newtonian substance and a blood sample received by the tube, prior to centrifuging the tube, and acts as a barrier between the second layer and the blood sample to prevent intermixing of the second layer and the blood sample. The thixotropic gel-like substance of the first layer has a specific gravity which is greater than the newtonian gel-like substance of the second layer whereupon centrifugation of the tube, the first layer moves to a position below the second layer. The second layer of the newtonian gel-like substance acts as a density separation medium, the newtonian gel-like substance being formed with a specific gravity such that the second layer is adapted to assume a position in the collection tube, upon centrifugation of the tube, between the mononuclear cells and the heavier components of the blood sample placed in the tube.
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公开(公告)号：US4816168A
公开(公告)日：1989-03-28
申请号：US923909
申请日：1986-10-28
CPC分类号： G01N33/491 , Y10T436/25375
摘要： This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
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9.发明授权Anticoagulant solution for use in blood chemistry-related techniques and apparatus 失效用于血液化学相关技术和装置的抗凝血剂溶液
公开(公告)号：US5667963A
公开(公告)日：1997-09-16
申请号：US603610
申请日：1996-02-21
申请人： Ward C. Smith , Richard L. Carroll
发明人： Ward C. Smith , Richard L. Carroll
CPC分类号： G01N33/5002 , G01N33/4905 , Y10T436/25375
摘要： An anticoagulant solution of sodium citrate for use in blood chemistry-related techniques and apparatus is disclosed. The anticoagulant solution should include an effective concentration of sodium citrate sufficient for preventing the coagulation of a sample of blood employed in the technique or added to the apparatus. The sodium citrate-based anticoagulant solution should have a pH ranging from above 6.0 to about 8.5 and a sodium citrate concentration preferably ranging from about 0.05M to about 0.2M.
摘要翻译： 公开了一种用于血液化学相关技术和装置中的柠檬酸钠抗凝血剂溶液。 抗凝血剂溶液应包括足够的有效浓度的柠檬酸钠，以防止该技术中使用的血液样品的凝结或添加到该装置中。 柠檬酸钠基抗凝血剂溶液的pH应在6.0至8.5之间，柠檬酸钠浓度优选为约0.05M至约0.2M。
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10.发明授权Apparatus for separating mononuclear cells from blood and method of manufacturing and using the same 失效用于从血液中分离单核细胞的装置及其制造方法及其使用方法
公开(公告)号：US4954264A
公开(公告)日：1990-09-04
申请号：US305953
申请日：1989-02-02
申请人： Ward C. Smith
发明人： Ward C. Smith
CPC分类号： G01N33/491 , B01D43/00 , B01L3/50215
摘要： A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated above and in contact with the second layer of gel-like substance, and a third layer of a Newtonian gel-like substance placed in the tube above the porous foam member. The gel-like substance of the second layer has a lower specific gravity than that of the liquid density gradient material of the first layer so that it floats on top of the first layer. The gel-like substance of the second layer becomes less viscous when heated such that it is absorbed by the porous foam member and forms a hydraulic barrier between the liquid density gradient material of the first layer and a blood sample placed in the collection tube.
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