公开(公告)号：US20110183372A1

公开(公告)日：2011-07-28

申请号：US13003661

申请日：2009-07-09

申请人： Richard John Stebbings

发明人： Richard John Stebbings

IPC分类号： G01N1/28

CPC分类号： G01N33/96

摘要： Methods for producing stimulated, positive and negative control reference standard for monitoring intracellular cytokine levels and cytokine release in test samples by stimulating cells to produce cytokines in the presence of a cytokine release inhibitor, fixing the stimulated cells with a fixative such as paraformaldehyde, washing to remove excess fixatives and freeze-drying the stimulated, fixed cells. Methods for producing labeled reference standards for cell proliferation assays are also disclosed, in which proliferation-competent mammalian cells, isolated from a human or animal body are labeled with a label, such as a dye, that is divided between daughter cells during cell proliferation (e.g., carboxyfluorescein succinimidyl ester), the cells are stimulated to proliferate, the proliferated cells are fixed by addition of a fixative and then preserved by freeze drying or cryopreservation.

摘要翻译： 用于产生用于通过刺激细胞在细胞因子释放抑制剂存在下产生细胞因子的细胞内细胞因子水平和细胞因子释放来监测细胞内细胞因子水平和细胞因子释放的方法，用固定剂如多聚甲醛固定刺激的细胞，洗涤至 去除多余的固定剂并冷冻干燥受刺激的固定细胞。 还公开了用于产生细胞增殖测定的标记参考标准的方法，其中从人或动物体分离的增殖能力的哺乳动物细胞用细胞增殖期间在子细胞之间划分的标记（例如染料）进行标记（ 例如羧基荧光素琥珀酰亚胺酯），刺激细胞增殖，通过加入固定剂固定增殖的细胞，然后通过冷冻干燥或冷冻保存来保存细胞。

公开(公告)号：US09267956B2

公开(公告)日：2016-02-23

申请号：US13003661

申请日：2009-07-09

申请人： Richard John Stebbings

发明人： Richard John Stebbings

IPC分类号： G01N33/96

CPC分类号： G01N33/96

摘要： Methods for producing stimulated, positive and negative control reference standard for monitoring intracellular cytokine levels and cytokine release in test samples by stimulating cells to produce cytokines in the presence of a cytokine release inhibitor, fixing the stimulated cells with a fixative such as paraformaldehyde, washing to remove excess fixatives and freeze-drying the stimulated, fixed cells. Methods for producing labeled reference standards for cell proliferation assays are also disclosed, in which proliferation-competent mammalian cells, isolated from a human or animal body are labeled with a label, such as a dye, that is divided between daughter cells during cell proliferation (e.g., carboxyfluorescein succinimidyl ester), the cells are stimulated to proliferate, the proliferated cells are fixed by addition of a fixative and then preserved by freeze drying or cryopreservation.

摘要翻译： 用于产生用于通过刺激细胞在细胞因子释放抑制剂存在下产生细胞因子的细胞内细胞因子水平和细胞因子释放来监测细胞内细胞因子水平和细胞因子释放的方法，用固定剂如多聚甲醛固定刺激的细胞，洗涤至 去除多余的固定剂并冷冻干燥受刺激的固定细胞。 还公开了用于产生细胞增殖测定的标记参考标准的方法，其中从人或动物体分离的增殖能力的哺乳动物细胞用细胞增殖期间在子细胞之间划分的标记（例如染料）进行标记（ 例如羧基荧光素琥珀酰亚胺酯），刺激细胞增殖，通过加入固定剂固定增殖的细胞，然后通过冷冻干燥或冷冻保存来保存细胞。

公开(公告)号：US20080233131A1

公开(公告)日：2008-09-25

申请号：US11575280

申请日：2005-09-13

申请人： Richard John Stebbings

发明人： Richard John Stebbings

IPC分类号： A61K39/395 ,  A61K39/00 ,  A61P37/00

CPC分类号： C07K16/2812 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/52

摘要： The invention provides a method for generating anti-CD4 auto-antibodies comprising administering to an individual an anti-CD4 antibody molecule which has a low affinity for native CD4 in said individual or a protein or peptide comprising a CD4-like epitope, wherein administration of said antibody molecule, protein or peptide results in the generation of anti-CD4 auto-antibodies.

摘要翻译： 本发明提供了一种产生抗CD4自身抗体的方法，包括向个体施用对所述个体对天然CD4具有低亲和力的抗CD4抗体分子或包含CD4样表位的蛋白质或肽，其中施用 所述抗体分子，蛋白质或肽导致抗CD4自身抗体的产生。