公开(公告)号：US5916801A

公开(公告)日：1999-06-29

申请号：US960529

申请日：1997-10-31

申请人： Richard William LoPinto ,  John Christopher Santelli

发明人： Richard William LoPinto ,  John Christopher Santelli

IPC分类号： G01N33/18 ,  C12M1/34 ,  C12Q1/20

CPC分类号： G01N33/186

摘要： A computer interfaced device for rapidly quantifying levels of toxicity to small aquatic organisms in aqueous environments. The device has a plurality of test chambers containing replicates of a plurality of concentrations of a substance or sample being tested for toxicity. Each chamber also contains a predetermined number of motile aquatic organisms. Light originating on one side of each test chamber is received by a photo-detector on the opposite side. The device's sensitivity is adjusted so that each interruption of a light beam caused by movement of a test organism in each test chamber is recorded for that chamber. Using this device and a toxicity testing protocol both lethality and changes in the frequency of test organism's movement in each concentration of substance or sample is monitored. Toxicity is by convention quantified as the concentration of a substance or sample which causes a predetermined level of lethal or sublethal response usually after a plurality of days. Using simple statistical software, the level of lethality which will occur following a plurality of days of exposure to each concentration can be predicted from motility data generated within just a few hours (usually 2.5 Hours) of exposing test organisms to the substance or sample. The computer includes software responsive to light beam interruption for deriving a set of parameters useful for predicting lethality. The device and accompanying software permits toxicity of a substance or sample to be quantified rapidly from motility data, long before lethality occurs.

摘要翻译： 一种计算机接口装置，用于在水环境中快速量化对小水生生物的毒性水平。 该装置具有多个测试室，其包含多个浓度的待测物质或样品的毒性的重复。 每个室还含有预定数量的活动水生生物。 在每个测试室的一侧产生的光由相对侧的光电检测器接收。 调整设备的灵敏度，以便记录每个测试室中的测试生物体的移动引起的光束的每次中断。 使用该设备和毒性测试方案，监测每种浓度物质或样品中测试生物运动频率的致死率和变化。 按照惯例将毒性定量为通常在多天后引起预定水平的致死或亚致死反应的物质或样品的浓度。 使用简单的统计软件，可以在将测试生物体暴露于物质或样品的短短几个小时（通常为2.5小时）内产生的运动数据来预测在每个浓度暴露多天后将发生的致死率水平。 计算机包括响应于光束中断的软件，用于得出用于预测致死率的一组参数。 该设备和随附的软件允许物质或样品的毒性从致死性数据快速量化，在致死性发生之前。