公开(公告)号：US20100226926A1

公开(公告)日：2010-09-09

申请号：US12720582

申请日：2010-03-09

申请人： Gregory C. Loney ,  Vikram Mohan ,  Rob Monroe ,  Bikash Sabata ,  Glenn Stark

发明人： Gregory C. Loney ,  Vikram Mohan ,  Rob Monroe ,  Bikash Sabata ,  Glenn Stark

IPC分类号： A61K39/395 ,  C12Q1/68

CPC分类号： C12Q1/6813

摘要： Disclosed herein, in certain embodiments, is a method of treating breast cancer characterized by the amplification of HER2 genes in a subject in need thereof, comprising: (a) isolating a tissue sample comprised of a plurality of breast tumor cells; (b) isolating a first section from said tissue sample; (c) isolating a second section from an adjacent portion of said tissue sample; (d) contacting the first section with a first stain; (e) contacting the second section with a probe; (f) imaging the first section following contact with the stain to produce a first image; (g) analyzing the first image for abnormal microscopic features; (h) identifying areas of interest in the first image that display abnormal microscopic features; (i) electronically annotating the first image to identify the areas of interest; (j) imaging the second section following contact with the probe; (k) aligning the first image and the second image; and (l) analyzing areas of interest in the second image that correspond to an area of interest identified in the first image; wherein the subject is administered an anti-HER2 antibody if HER2 is amplified or providing an alternative treatment if HER2 is not amplified.

摘要翻译： 在某些实施方案中，本文公开了一种治疗乳腺癌的方法，其特征在于在有需要的受试者中扩增HER2基因，其包括：（a）分离由多个乳腺肿瘤细胞组成的组织样品; （b）从所述组织样品中分离第一部分; （c）从所述组织样本的相邻部分分离第二部分; （d）使第一部分与第一染料接触; （e）使第二部分与探针接触; （f）在与染色剂接触之后成像第一部分以产生第一图像; （g）分析第一图像的异常显微特征; （h）识别显示异常微观特征的第一图像中的感兴趣区域; （i）以电子方式注释第一张图片以确定感兴趣的区域; （j）在与探针接触之后成像第二部分; （k）对准所述第一图像和所述第二图像; 和（l）分析对应于在第一图像中标识的兴趣区域的第二图像中的感兴趣区域; 其中如果HER2被扩增，则所述受试者被施用抗HER2抗体，或者如果HER2不被扩增则提供替代治疗。