公开(公告)号：US06608035B1

公开(公告)日：2003-08-19

申请号：US09587934

申请日：2000-06-06

申请人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

发明人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

IPC分类号： A01N4304

CPC分类号： C12N15/113 ,  A61K31/7125 ,  A61K38/1709 ,  C12N2310/312 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/345 ,  Y02A50/411 ,  Y02A50/491

摘要： Disclosed is a method of down-regulating the expression of a gene in an animal, wherein a pharmacological formulation comprising a chimeric oligonucleotide complementary to the gene is orally administered to an animal. The oligonucleotide administered has at least one phosphorothioate internucleotide linkage and at least one alkylphosphonate, phosphorodithioate, alkylphosphonothioate, phosphoramidate, phosphoramidite, phosphate ester, carbamate, carbonate, phosphate triester, acetamidate, or carboxymethyl ester internucleotide linkage.

摘要翻译： 公开了一种下调动物基因表达的方法，其中将包含与该基因互补的嵌合寡核苷酸的药理学制剂口服给予动物。 所施用的寡核苷酸具有至少一个硫代磷酸酯核苷酸间键和至少一种烷基膦酸盐，二硫代磷酸盐，烷基磷硫酸盐，氨基磷酸盐，亚磷酰胺，磷酸酯，氨基甲酸盐，碳酸盐，磷酸三酯，乙酰胺盐或羧甲基酯核苷酸间键。

公开(公告)号：US5591721A

公开(公告)日：1997-01-07

申请号：US328520

申请日：1994-10-25

申请人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

发明人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

IPC分类号： C12N15/09 ,  A61K20060101 ,  A61K31/70 ,  A61K31/795 ,  A61K48/00 ,  A61P25/28 ,  A61P31/12 ,  A61P33/00 ,  A61P33/10 ,  A61P35/00 ,  C07H21/00 ,  C07H21/04

CPC分类号： A61K31/7125

摘要： Disclosed is a method of down-regulating the expression of a gene in an animal, wherein a pharmalogical formulation comprising an oligonucleotide complementary to the gene is orally administered to an animal. The oligonucleotide administered has non-phosphodiester internucleotide linkages and includes at least one 2'-substituted ribonucleotide, the oligonucleotide inhibiting the expression of a product of the gene, thereby down-regulating the expression of the gene.

摘要翻译： 公开了一种下调动物基因表达的方法，其中向动物口服施用包含与该基因互补的寡核苷酸的药用制剂。 所施用的寡核苷酸具有非磷酸二酯核苷酸间键，并且包括至少一个2'-取代的核糖核苷酸，该寡核苷酸抑制该基因产物的表达，从而下调该基因的表达。

公开(公告)号：US06936593B1

公开(公告)日：2005-08-30

申请号：US09777526

申请日：2001-02-06

申请人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

发明人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

IPC分类号： A01N43/04 ,  A61K31/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12P19/34 ,  C12Q1/68

CPC分类号： A61K31/7125 ,  Y02A50/387 ,  Y02A50/402 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/423

摘要： Disclosed is a method of down-regulating the expression of a gene in an animal, wherein a pharmacological formulation comprising a chimeric oligonucleotide complementary to the gene is orally administered to an animal. The oligonucleotide administered has at least one phosphorothioate internucleotide linkage and at least one alkylphosphonate, phosphorodithioate, alkylphosphonothioate, phosphoramidate, phosphoramidite, phosphate ester, carbamate, carbonate, phosphate triester, acetamidate, or carboxymethyl ester internucleotide linkage.

摘要翻译： 公开了一种下调动物基因表达的方法，其中将包含与该基因互补的嵌合寡核苷酸的药理学制剂口服给予动物。 所施用的寡核苷酸具有至少一个硫代磷酸酯核苷酸间键和至少一种烷基膦酸盐，二硫代磷酸盐，烷基磷硫酸盐，氨基磷酸盐，亚磷酰胺，磷酸酯，氨基甲酸盐，碳酸盐，磷酸三酯，乙酰胺盐或羧甲基酯核苷酸间键。

公开(公告)号：US06645943B1

公开(公告)日：2003-11-11

申请号：US09321249

申请日：1999-05-27

申请人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

发明人： Sudhir Agrawal ,  Robert B. Diasio ,  Ruiwen Zhang

IPC分类号： A61K3100

CPC分类号： A61K31/7125 ,  Y02A50/387 ,  Y02A50/402 ,  Y02A50/409 ,  Y02A50/411 ,  Y02A50/414 ,  Y02A50/423

摘要： Disclosed is a method of down-regulating the expression of a gene in an animal, wherein a pharmacological formulation comprising a chimeric oligonucleotide complementary to the gene is orally administered to an animal. The oligonucleotide administered has at least one phosphorothioate internucleotide linkage and at least one alkylphosphonate, phosphorodithioate, alkylphosphonothioate, phosphoramidate, phosphoramidite, phosphate ester, carbamate, carbonate, phosphate triester, acetamidate, or carboxymethyl ester internucleotide linkage.