公开(公告)号：US07442787B2

公开(公告)日：2008-10-28

申请号：US11087052

申请日：2005-03-22

申请人： Michael Antoniou ,  Robert Crombie

发明人： Michael Antoniou ,  Robert Crombie

IPC分类号： C12N15/00

CPC分类号： C12N15/11 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/021 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2830/008 ,  C12N2830/205 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE). The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleotide, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.

摘要翻译： 本发明涉及包含普遍存在的染色质开放元件（UCOE）的多核苷酸。 本发明还涉及包含多核苷酸序列的载体，包含载体的宿主细胞，治疗和测定中多核苷酸，载体或宿主细胞的用途以及鉴定UCOE的方法。 UCOE打开染色质或维持染色质处于开放状态，并且促进可操作地连接的基因在至少两种不同组织类型的细胞中的表达。

公开(公告)号：US20060161968A1

公开(公告)日：2006-07-20

申请号：US11312652

申请日：2005-12-21

申请人： Robert Crombie ,  Patrick Link ,  Ben Ong ,  David McCarten

发明人： Robert Crombie ,  Patrick Link ,  Ben Ong ,  David McCarten

IPC分类号： H04L9/32

CPC分类号： G06F21/10 ,  G06F2221/2109

摘要： Prototype software is securely delivered and evaluated by electronic transfer over a network. The software is secured by multiple levels of encryption to prevent unauthorized copying, modification, and/or use of the prototype software. Electronic transfer of the prototype software minimizes the time and cost associated with providing prototype software for testing to remote third party testers. Once the software has been transferred electronically to the third party tester, the software developer electronically maintains control of that software by restricting access to an authorized third party, monitoring testing, and deleting any files related to the prototype software from the third party test workstation.

摘要翻译： 原型软件通过电子网络传输安全地传递和评估。 该软件通过多级加密来保护，以防止未经授权的复制，修改和/或使用原型软件。 原型软件的电子传输最大限度地减少了提供用于向远程第三方测试人员测试的原型软件所需的时间和成本。 一旦软件被电子传送到第三方测试人员，软件开发商通过限制对授权的第三方的访问，监视测试以及从第三方测试工作站删除与原型软件相关的任何文件来电子地维护该软件的控制。

公开(公告)号：US06964951B2

公开(公告)日：2005-11-15

申请号：US10225418

申请日：2002-08-21

申请人： Michael Antoniou ,  Robert Crombie

发明人： Michael Antoniou ,  Robert Crombie

IPC分类号： A61K48/00 ,  C12N1/21 ,  C12N15/11 ,  C12N15/85 ,  A61K31/713

CPC分类号： C12N15/11 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/021 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2830/008 ,  C12N2830/205 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleoticle, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.

摘要翻译： 本发明涉及包含不衍生自13的普遍存在的染色质开放元件（UCOE）的多核苷酸。本发明还涉及包含多核苷酸序列的载体，包含载体的宿主细胞，使用多核苷酸，载体或 宿主细胞在治疗和测定中，以及鉴定UCOEs的方法。 UCOE打开染色质或维持染色质处于开放状态，并且促进可操作地连接的基因在至少两种不同组织类型的细胞中的表达。

公开(公告)号：US06689606B2

公开(公告)日：2004-02-10

申请号：US09358082

申请日：1999-07-21

申请人： Michael Antoniou ,  Robert Crombie

发明人： Michael Antoniou ,  Robert Crombie

IPC分类号： C12N510

CPC分类号： C12N15/11 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/021 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2830/008 ,  C12N2830/205 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from an LCR. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleotide, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.

摘要翻译： 本发明涉及包含不属于LCR的普遍存在的染色质开放元件（UCOE）的多核苷酸。 本发明还涉及包含多核苷酸序列的载体，包含载体的宿主细胞，治疗和测定中多核苷酸，载体或宿主细胞的用途以及鉴定UCOE的方法。 UCOE打开染色质或维持染色质处于开放状态，并且促进可操作地连接的基因在至少两种不同组织类型的细胞中的表达。

公开(公告)号：US20050181428A1

公开(公告)日：2005-08-18

申请号：US11087052

申请日：2005-03-22

申请人： Michael Antoniou ,  Robert Crombie

发明人： Michael Antoniou ,  Robert Crombie

IPC分类号： A61K48/00 ,  C12N1/21 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22

CPC分类号： C12N15/11 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/021 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2830/008 ,  C12N2830/205 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The polynucleotide of any one of claims 1 to 7, wherein the UCOE comprises the sequence of FIG. 20 between nucleotides 1 to 7627 or a functional homologue or fragment thereof an LCR. The present inverition also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleoticle, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.

摘要翻译： 本发明涉及包含不衍生自13的普遍存在的染色质开放元件（UCOE）的多核苷酸。8.权利要求1至7中任一项的多核苷酸，其中所述UCOE包含图1的序列。 20个核苷酸1至7627之间或其功能同源物或片段的LCR。 本逆转还涉及包含多核苷酸序列的载体，包含载体的宿主细胞，治疗和测定中多核苷酸，载体或宿主细胞的用途以及鉴定UCOE的方法。 UCOE打开染色质或维持染色质处于开放状态，并且促进可操作地连接的基因在至少两种不同组织类型的细胞中的表达。

公开(公告)号：US06881556B2

公开(公告)日：2005-04-19

申请号：US10225073

申请日：2002-08-21

申请人： Michael Antoniou ,  Robert Crombie

发明人： Michael Antoniou ,  Robert Crombie

IPC分类号： A61K48/00 ,  C12N1/21 ,  C12N15/11 ,  C12N15/85 ,  C12P21/02

CPC分类号： C12N15/11 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/85 ,  C12N2799/021 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2830/008 ,  C12N2830/205 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from 13. The polynucleotide of any one of claims 1 to 7, wherein the UCOE comprises the sequence of FIG. 20 between nucleotides 1 to 7627 or a functional homologue or fragment thereof. an LCR. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleotide, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.

摘要翻译： 本发明涉及包含不衍生自13的普遍存在的染色质开放元件（UCOE）的多核苷酸。8.权利要求1至7中任一项的多核苷酸，其中所述UCOE包含图1的序列。 20个核苷酸1至7627之间或其功能同系物或其片段。 一个LCR。 本发明还涉及包含多核苷酸序列的载体，包含载体的宿主细胞，治疗和测定中多核苷酸，载体或宿主细胞的用途以及鉴定UCOE的方法。 UCOE打开染色质或维持染色质处于开放状态，并且促进可操作地连接的基因在至少两种不同组织类型的细胞中的表达。