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公开(公告)号：US09127043B2
公开(公告)日：2015-09-08
申请号：US13254697
申请日：2010-03-04
申请人： Robert S. Gronke , Héctor Zaanoni
发明人： Robert S. Gronke , Héctor Zaanoni
CPC分类号： C07K1/22 , B01D15/26 , B01D15/3804 , B01D15/3828 , B01D2015/3838 , C07K1/165
摘要： The present invention pertains to improved methods of using dye-ligand affinity chromatography for the isolation of antibodies or proteins comprising an antibody fragment (such as Fc fusion proteins) from a mixture of undesirable contaminants. In particular, the use of an organic polymer such as polyethylene glycol (PEG) in the elution phase of an antibody/dye-ligand chromatography isolation procedure results in improved separation of target antibodies from undesirable contaminants. The methods described herein are particularly useful in separating or removing antibody aggregates, misfolded antibodies, and virus contaminants from target antibodies.
摘要翻译： 本发明涉及使用染料 - 配体亲和层析从不想要的污染物的混合物分离包含抗体片段（例如Fc融合蛋白）的抗体或蛋白质的改进方法。 特别地，在抗体/染料 - 配体色谱分离方法的洗脱阶段中使用有机聚合物如聚乙二醇（PEG）导致靶抗体与不期望的污染物的分离改善。 本文描述的方法特别可用于从靶抗体中分离或去除抗体聚集体，错误折叠的抗体和病毒污染物。
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公开(公告)号：US20120053325A1
公开(公告)日：2012-03-01
申请号：US13254697
申请日：2010-03-04
申请人： Robert S. Gronke , Héctor Zaanoni
发明人： Robert S. Gronke , Héctor Zaanoni
CPC分类号： C07K1/22 , B01D15/26 , B01D15/3804 , B01D15/3828 , B01D2015/3838 , C07K1/165
摘要： The present invention pertains to improved methods of using dye-ligand affinity chromatography for the isolation of antibodies or proteins comprising an antibody fragment (such as Fc fusion proteins) from a mixture of undesirable contaminants. In particular, the use of an organic polymer such as polyethylene glycol (PEG) in the elution phase of an antibody/dye-ligand chromatography isolation procedure results in improved separation of target antibodies from undesirable contaminants. The methods described herein are particularly useful in separating or removing antibody aggregates, misfolded antibodies, and virus contaminants from target antibodies.
摘要翻译： 本发明涉及使用染料 - 配体亲和层析从不想要的污染物的混合物分离包含抗体片段（例如Fc融合蛋白）的抗体或蛋白质的改进方法。 特别地，在抗体/染料 - 配体色谱分离方法的洗脱阶段中使用有机聚合物如聚乙二醇（PEG）导致目标抗体与不期望的污染物的分离改善。 本文描述的方法特别可用于从靶抗体中分离或去除抗体聚集体，错误折叠的抗体和病毒污染物。
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3.发明申请Method of Isolating Biomacromolecules Using Polyalkylene Glycol and Transition Metals 审中-公开使用聚亚烷基二醇和过渡金属分离生物大分子的方法公开(公告)号：US20090292109A1
公开(公告)日：2009-11-26
申请号：US12425328
申请日：2009-04-16
CPC分类号： C07K1/30 , A61K39/39591 , C07K16/00 , C07K2317/14
摘要： The present invention is related to a method of isolating a biological macromolecule in a composition. Specifically, the present invention is related to a method of isolating a biomacromolecule in a composition containing an impurity, the method comprising adding a polyalkylene glycol to the composition, adding a transition metal to the composition, and separating said biomacromolecule from said impurity.
摘要翻译： 本发明涉及在组合物中分离生物大分子的方法。 具体地，本发明涉及在含有杂质的组合物中分离生物大分子的方法，该方法包括向组合物中加入聚亚烷基二醇，向组合物中加入过渡金属，并将所述生物大分子与所述杂质分离。
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