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1.发明授权Microtitration plates comprising wells coated with a lipid substrate and a method of preparing 失效包含涂有脂质底物的孔的微量滴定板和制备方法公开(公告)号：US08148096B2
公开(公告)日：2012-04-03
申请号：US11884139
申请日：2006-02-10
IPC分类号： C12Q1/34
CPC分类号： C12Q1/34 , B01J2219/00315 , B01J2219/00603 , B01J2219/00659 , B01J2219/00734 , B01L3/5085 , B01L2200/16 , B01L2300/0829 , B01L2300/16 , G01N2333/918 , G01N2405/02
摘要： A method for the detection and/or measurement in vitro of a lipase or phospholipase activity, including the addition of a sample likely to contain said lipase or phospholipase into the wells of microtitration plates coated with a layer of a lipid substrate which is able to be hydrolyzed by the lipase or phospholipase by releasing α-eleostearic acid, and the detection and/or the measurement of the lipase or phospholipase activity by UV spectrophotometry of the α-eleostearic acid released during the previous stage. The application of this method to the in vitro diagnosis of pathologies linked to an increase in the plasma lipase level.
摘要翻译： 用于体外检测和/或测量脂肪酶或磷脂酶活性的方法，包括将可能含有所述脂肪酶或磷脂酶的样品添加到涂覆有脂质底物层的微量滴定板的孔中，该层可以是 通过释放α-蒿酸酯由脂肪酶或磷脂酶水解，通过前一阶段释放的α-桐酸的紫外分光光度法检测和/或测量脂肪酶或磷脂酶活性。 该方法的应用与体外诊断病理学有关，血浆脂肪酶水平升高。
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2.发明授权Lipases and lipase extracts, their preparation process and their therapeutic use 失效脂肪和脂肪提取物，其制备方法及其治疗用途
公开(公告)号：US5075231A
公开(公告)日：1991-12-24
申请号：US97896
申请日：1987-09-17
申请人： Herve Moreau , Robert Verger , Daniel Lecat , Jean-Louis Junien
发明人： Herve Moreau , Robert Verger , Daniel Lecat , Jean-Louis Junien
摘要： Process for the preparation of a gastric lipase. The stomachs of rabbits or horses undergo extraction by an acid aqueous medium, the lipase extract is salted out by the addition of hydrosoluble salts then, by filtration on a molecular sieve, followed by separation using ion exchange chromatography, an elution fraction containing the lipase is collected.Medicament for fighting against malabsorptions of fatty substances.
摘要翻译： 胃脂肪酶的制备方法。 兔子或马的胃通过酸性水性介质进行提取，通过添加水溶性盐将脂肪酶提取物盐析，然后通过在分子筛上过滤，然后使用离子交换层析分离，含有脂肪酶的洗脱级分为 集。 用于防止脂肪物质吸收不良的药物。
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3.发明申请Method for the High-Speed Detection and/or Measurement of a Lipase or Phospholipase Activity 失效高速检测和/或测量脂肪酶或磷脂酶活性的方法公开(公告)号：US20080193958A1
公开(公告)日：2008-08-14
申请号：US11884139
申请日：2006-02-10
CPC分类号： C12Q1/34 , B01J2219/00315 , B01J2219/00603 , B01J2219/00659 , B01J2219/00734 , B01L3/5085 , B01L2200/16 , B01L2300/0829 , B01L2300/16 , G01N2333/918 , G01N2405/02
摘要： The invention relates to a method for the detection and/or measurement in vitro of a lipase or phospholipase activity, characterized in that it comprises: the addition of a sample likely to contain said lipase or phospholipase into the wells of microtitration plates coated with a layer of a lipid substrate which is able to be hydrolyzed by said lipase or phospholipase by releasing α-eleostearic acid, the detection and/or the measurement of the lipase or phospholipase activity by UV spectrophotometry of the α-eleostearic acid released during the previous stage. The invention also relates to the application of the above-mentioned method to the in vitro diagnosis of pathologies linked to an increase in the plasma lipase level.
摘要翻译： 本发明涉及用于体外检测和/或测量脂肪酶或磷脂酶活性的方法，其特征在于其包括：将可能含有所述脂肪酶或磷脂酶的样品加入涂覆有层的微量滴定板的孔中 的脂质底物，其能够通过释放α-桐酸被所述脂肪酶或磷脂酶水解，通过前一阶段释放的α-桐酸的紫外分光光度法检测和/或测量脂肪酶或磷脂酶活性。 本发明还涉及上述方法用于与血浆脂肪酶水平增加相关的病理学的体外诊断的应用。
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4.发明授权
公开(公告)号：US5479816A
公开(公告)日：1996-01-02
申请号：US117177
申请日：1994-04-22
申请人： Jacques Richou , Michel Grimaldi , Robert Verger , Claude Riviere , Andre Bois , Sylvie Nury
发明人： Jacques Richou , Michel Grimaldi , Robert Verger , Claude Riviere , Andre Bois , Sylvie Nury
IPC分类号： G01N13/02
CPC分类号： G01N13/02 , G01N2013/0241 , G01N2013/0275
摘要： Process and apparatus for analyzing the characteristics of a liquid/fluid interface, especially in the presence of surface-active agents. A tensiometer operates according to the principle of the suspended drop and the amount of liquid injected in the drop is adjusted according to the value of the calculated interface tension; the volume of the formed drop is progressively increased as the interface tension tends to diminish. A drop-forming device (30) can acquire an image of the drop and read the drop contour (40), can calculate the interface tension based on the shape and the size of the contour, and can adjust the same. A method of high speed measurement of interface tension uses all the points in the drop contour and provides a value in less than a second with the aid of a microcomputer.
摘要翻译： PCT No.PCT / FR92 / 00227 Sec。 371日期1994年04月22日 102（e）日期1994年4月22日PCT 1991年3月12日PCT公布。 出版物WO92 / 16824 日期1992年10月1日。用于分析液体/流体界面的特性的处理和装置，特别是在存在表面活性剂的情况下。 张力计根据悬浮液的原理进行操作，并根据计算的界面张力的值调整滴入液中的液体量; 当界面张力趋于减小时，形成的液滴的体积逐渐增加。 液滴形成装置（30）可以获取液滴的图像并读取液滴轮廓（40），可以基于轮廓的形状和尺寸计算界面张力，并且可以进行调节。 高速测量接口张力的方法使用液滴轮廓中的所有点，并借助于微型计算机在不到一秒的时间内提供一个值。
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