公开(公告)号：US5942388A

公开(公告)日：1999-08-24

申请号：US390978

申请日：1995-02-21

申请人： Itamar Willner ,  Arie Dagan ,  Shai Rubin ,  Ron Blonder ,  Azalia Riklin ,  Yael Cohen

发明人： Itamar Willner ,  Arie Dagan ,  Shai Rubin ,  Ron Blonder ,  Azalia Riklin ,  Yael Cohen

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/416 ,  G01N33/543 ,  G01N33/552 ,  G01N33/553

CPC分类号： C12Q1/004 ,  C12Q1/003 ,  G01N33/5438 ,  G01N33/552 ,  G01N33/553 ,  Y10S435/962 ,  Y10S436/806 ,  Y10S436/825

摘要： An elcctrobiochemical system for the determination of the presence and optionally concentration of an analyte in a liquid medium, the system comprising an electrode having immobilized thereon a member of a recognition pair, the other member of said pair being said analyte, the presence of said analyte in the medium resulting in formation of a pair complex, being a complex between said immobilized member and said analyte; the system further comprising redox molecules capable of changing their redox state by accepting electrons from or donating electrons to the electrode; the formation of the pair complex on the electrode bringing a change in the electrical response of the system, whereby the presence and optionally the concentration of said analyte in the medium can be determined.

摘要翻译： 一种用于确定液体介质中分析物的存在和任选浓度的电生化系统，该系统包括固定有识别对的构件的电极，所述一对的另一个成员是所述分析物，所述分析物的存在 在介质中导致形成一对复合物，其是所述固定化构件和所述分析物之间的复合物; 该系统还包含能够通过从电极接受电子或向电极提供电子而能够改变其氧化还原状态的氧化还原分子; 在电极上形成配对络合物，从而改变系统的电响应，从而可以确定介质中所述分析物的存在和任选的浓度。

公开(公告)号：US06350368B1

公开(公告)日：2002-02-26

申请号：US09194364

申请日：1999-06-30

申请人： Itamar Willner ,  Eugeny Katz ,  Ron Blonder ,  Vered Heleg-Shabtai

发明人： Itamar Willner ,  Eugeny Katz ,  Ron Blonder ,  Vered Heleg-Shabtai

IPC分类号： G01N2726

CPC分类号： C12Q1/004

摘要： Electrodes carrying FAD-dependent enzymes on their surface are enclosed. The FAD is modified to include a functional group or moiety which affects the properties of the enzyme. The functional group or moiety can be a binding moiety through which the FAD-enzyme complex is immobilized onto the electrode; it can be an electron mediator group for transferring electrons between the electrode and the FAD; or it can be a photoisomerizable group which can undergo photoisomerization which yields a change in the rate of the electrically induced catalytic activity of the enzyme. The electrodes can be used in electrochemical systems for deforming analytes in liquid medium or for recordal of optical signals.

摘要翻译： 封装在其表面携带FAD依赖性酶的电极。 FAD被修饰为包括影响酶的性质的官能团或部分。 官能团或部分可以是结合部分，通过其将FAD-酶复合物固定在电极上; 它可以是用于在电极和FAD之间传递电子的电子介体组; 或者它可以是可以进行光异构化的光可异构化基团，其产生酶的电诱导催化活性的速率的变化。 电极可用于电化学系统，用于使液体介质中的分析物变形或者用于记录光信号。

公开(公告)号：US06214205B1

公开(公告)日：2001-04-10

申请号：US09117256

申请日：1998-12-15

申请人： Itamar Willner ,  Eugenii Katz ,  Yael Cohen ,  Ron Blonder

发明人： Itamar Willner ,  Eugenii Katz ,  Yael Cohen ,  Ron Blonder

IPC分类号： G01N2726

CPC分类号： C12Q1/003 ,  G01N33/5438

摘要： Electrochemical determination of the presence, and optionally concentration of an analyte utilizes an electrode having immobilized thereon one member of a recognition pair, the recognition pair consisting of said analyte. In the presence of the analyte medium pair complexes, which are complexes between the analyte and the immobilized member are formed. The electrode forms part of an electrochemical system which includes also redox molecules comprising at least one first redox molecule and at least one second redox molecule, the former transferring electrons between the electrode and the latter. Upon formation of a pair complex the electron transfer is inhibited and the change in the electrical response then indicates the presence of said analyte in the medium.

摘要翻译： 分析物的存在和任选地浓度的电化学测定使用其上固定有识别对的一个成员的电极，识别对由所述分析物组成。 在存在分析物的情况下，形成了分析物与固定化部件之间的复合物。 电极形成电化学系统的一部分，其还包括包含至少一个第一氧化还原分子和至少一个第二氧化还原分子的氧化还原分子，前者在电极和后者之间转移电子。 在形成一对复合物时，电子转移被抑制，并且电响应的变化然后指示所述分析物在介质中的存在。