公开(公告)号：US5443701A

公开(公告)日：1995-08-22

申请号：US109922

申请日：1993-08-23

申请人： Itamar Willner ,  Evgeny Katz ,  Azalia Riklin ,  Ron Kasher ,  Benjamin Shoham

发明人： Itamar Willner ,  Evgeny Katz ,  Azalia Riklin ,  Ron Kasher ,  Benjamin Shoham

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/004 ,  C12Q1/003 ,  Y10S435/817

摘要： An analytical method for determining the presence or concentration of an analyte in a liquid medium is provided in which electrons are transferred from an electrode material of an electrode to the redox center of an enzyme, by the mediation of an electron mediator, whereby in the presence of an analyte the enzyme calalyzed a redox reaction in which the analyte is converted into a product. By measuring either the concentration or the product or the charge which flows in this process, the presence and/or concentration of the analyte in the medium is determined. Either or both of the enzyme and the electron mediator are immobilized on the electrode.

摘要翻译： 提供了一种用于确定液体介质中分析物的存在或浓度的分析方法，其中电子通过电子介体的介导从电极的电极材料转移到酶的氧化还原中心，由此在存在 的分析物，酶催化氧化还原反应，其中分析物转化成产物。 通过测量在该过程中流动的浓度或产物或电荷，确定介质中分析物的存在和/或浓度。 酶和电子介体中的任一个或两者被固定在电极上。

公开(公告)号：US5942388A

公开(公告)日：1999-08-24

申请号：US390978

申请日：1995-02-21

申请人： Itamar Willner ,  Arie Dagan ,  Shai Rubin ,  Ron Blonder ,  Azalia Riklin ,  Yael Cohen

发明人： Itamar Willner ,  Arie Dagan ,  Shai Rubin ,  Ron Blonder ,  Azalia Riklin ,  Yael Cohen

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/416 ,  G01N33/543 ,  G01N33/552 ,  G01N33/553

CPC分类号： C12Q1/004 ,  C12Q1/003 ,  G01N33/5438 ,  G01N33/552 ,  G01N33/553 ,  Y10S435/962 ,  Y10S436/806 ,  Y10S436/825

摘要： An elcctrobiochemical system for the determination of the presence and optionally concentration of an analyte in a liquid medium, the system comprising an electrode having immobilized thereon a member of a recognition pair, the other member of said pair being said analyte, the presence of said analyte in the medium resulting in formation of a pair complex, being a complex between said immobilized member and said analyte; the system further comprising redox molecules capable of changing their redox state by accepting electrons from or donating electrons to the electrode; the formation of the pair complex on the electrode bringing a change in the electrical response of the system, whereby the presence and optionally the concentration of said analyte in the medium can be determined.

摘要翻译： 一种用于确定液体介质中分析物的存在和任选浓度的电生化系统，该系统包括固定有识别对的构件的电极，所述一对的另一个成员是所述分析物，所述分析物的存在 在介质中导致形成一对复合物，其是所述固定化构件和所述分析物之间的复合物; 该系统还包含能够通过从电极接受电子或向电极提供电子而能够改变其氧化还原状态的氧化还原分子; 在电极上形成配对络合物，从而改变系统的电响应，从而可以确定介质中所述分析物的存在和任选的浓度。