SEMI-AUTOMATED DEVICE FOR SINGLE PARAMETER AND MULTI-PARAMETER PHENOTYPING OF CELLS
    3.
    发明申请
    SEMI-AUTOMATED DEVICE FOR SINGLE PARAMETER AND MULTI-PARAMETER PHENOTYPING OF CELLS 审中-公开
    用于单个参数的半自动装置和多个参数的细胞反应

    公开(公告)号:US20110300618A1

    公开(公告)日:2011-12-08

    申请号:US13081593

    申请日:2011-04-07

    IPC分类号: C12M1/34

    摘要: A single-use device that produces a readily interpretable, visual presentation of biomaterial, enabling single parameter and multi-parameter characterizing of said biomaterial by means of visual inspection, particularly for immunophenotyping. In one embodiment, the device uses antibody coated microspheres which are adapted to bind to specific types of cells. An arrangement of uniquely molded components, which may be plastic or other suitable material, are assembled so as to provide a sequential procedure capable of incubating a suspension comprised of antibody coated microspheres, biomaterial, and liquid suspension agents. Following incubation, the invention further removes liquids from said suspension, and provides for rinsing the incubated retentate and for removing said rinsing liquids from the incubated retentate. The invention further provides for affixing the incubated retentate onto a standard glass microscope slide to enable phenotyping of incubated biomaterial (e.g., cells) by means of visual inspection or other analyses that can be performed from a glass slide.

    摘要翻译: 产生生物材料易于解释,可视化呈现的单次使用装置,通过目视检查,特别是免疫表型分析,可实现所述生物材料的单参数和多参数特征。 在一个实施方案中,该装置使用适于结合特定类型细胞的抗体包被的微球。 组装独特模制部件(可以是塑料或其它合适的材料)的布置被组装以提供能够孵育由抗体包被的微球,生物材料和液体悬浮剂组成的悬浮液的顺序程序。 在温育之后,本发明还从所述悬浮液中除去液体,并提供漂洗培养的滞留物并从培养的滞留物中除去所述漂洗液体。 本发明进一步提供将培养的滞留物固定在标准玻璃显微镜载玻片上,以便通过目视检查或可以从载玻片进行的其他分析来表达孵育的生物材料(例如,细胞)。