公开(公告)号：US06613535B1

公开(公告)日：2003-09-02

申请号：US07968553

申请日：1992-10-29

申请人： Joachim Albrecht ,  Frank Hulstaert ,  Rosette Becker

发明人： Joachim Albrecht ,  Frank Hulstaert ,  Rosette Becker

IPC分类号： G01N3353

CPC分类号： G01N33/5094 ,  G01N15/14 ,  G01N33/56977 ,  Y10S435/967 ,  Y10S435/968 ,  Y10S436/805 ,  Y10S436/811 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664

摘要： This invention relates to a method for establishing and using a decision marker by which positive samples can be discriminated from negative samples. The method employs the analysis of multiple samples from confirmed positive and negative samples. A fluorescence channel is selected so that the desired sensitivity and specificity are achieved. A microparticle having this fluorescence channel then is made and is used in conjunction with a fluorescence marker which is specific for the population of interest.

摘要翻译： 本发明涉及一种用于建立和使用决定标记的方法，通过该方法可以将正样本与负样本区分开来。 该方法采用从确认的阳性和阴性样本中分析多个样本。 选择荧光通道，使得实现所需的灵敏度和特异性。 然后制备具有该荧光通道的微粒，并与与感兴趣群体特异的荧光标记物结合使用。

公开(公告)号：US4818677A

公开(公告)日：1989-04-04

申请号：US128257

申请日：1987-12-03

申请人： Martha L. Hay-Kaufman ,  Rosette Becker ,  Robert Danisch

发明人： Martha L. Hay-Kaufman ,  Rosette Becker ,  Robert Danisch

IPC分类号： G01N33/543 ,  G01N33/76 ,  G01N33/53 ,  G01N33/569

CPC分类号： G01N33/54366 ,  G01N33/76 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/81 ,  Y10S436/813

摘要： Methods and kits are described for performing immunoassays. The kit includes a reaction cell having a microporous membrane and an absorbent capable of drawing liquid sample through the membrane. An applicator is provided for applying a small volume of the liquid sample to the membrane by contacting a port of the applicator to the membrane and allowing the sample to flow therethrough by capillary action. Analyte in the sample is immobilized, typically by immunoadsorption, and the immobilized analyte may then be visualized using conventional signal producing systems, such as color, fluorescence, and luminescent systems.