公开(公告)号：US06294366B1

公开(公告)日：2001-09-25

申请号：US09136574

申请日：1998-08-19

申请人： Graham K. Farrington ,  Paige Anderson ,  Peter Bergquist ,  Roy Daniels ,  Moreland David Gibbs ,  Hugh Morgan ,  Diane P. Williams

发明人： Graham K. Farrington ,  Paige Anderson ,  Peter Bergquist ,  Roy Daniels ,  Moreland David Gibbs ,  Hugh Morgan ,  Diane P. Williams

IPC分类号： C12N942

CPC分类号： C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/13 ,  D06P5/15

摘要： Alkalophilic and thermophilic cellulases having high stability to elevated temperatures and pH have been isolated from an organism of unknown species, which most closely resembles those in the Caldicellulosiruptor genus and which has been called by us, Tok7B.1, These cellulases have been cloned and expressed in a recombinant system, so that they can be produced in quantity. These are particularly useful in treating cellulosic materials including cotton-containing fabrics, as detergent additives, and in aqueous compositions. We also provide genomic DNA which can be used in recombinant expression vectors and expression systems to produce enhanced alkali and/or temperature stability properties in cellulases other than those specifically described.

摘要翻译： 已经从未知物种的生物体中分离出对升高的温度和pH具有高稳定性的嗜碱性和嗜热纤维素酶，其与Caldicellulosiruptor属中的那些非常相似，并且被我们称之为Tok7B.1。这些纤维素酶已被克隆和表达 在重组体系中，使得它们可以量产。 这些在处理包括含棉织物，作为洗涤剂添加剂的纤维素材料和水性组合物中特别有用。 我们还提供可用于重组表达载体和表达系统的基因组DNA，以在除了具体描述的纤维素酶之外的纤维素酶中产生增强的碱和/或温度稳定性。