公开(公告)号：US20070148657A1

公开(公告)日：2007-06-28

申请号：US11322157

申请日：2005-12-28

Applicant: Joel Myerson ,  Paige Anderson

Inventor： Joel Myerson ,  Paige Anderson

IPC: C12Q1/68 ,  C12M3/00

CPC classification number: C12Q1/6832 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2565/119 ,  C12Q2527/125

Abstract: The present document relates to a system and method of commingling a low volume of a target solution with an array. For example, a quantity of the target solution and a quantity of an immiscible liquid may be introduced into a cavity having an array on an interior surface. The quantities of target solution and immiscible liquid do not fill the volume. Therefore, a gas is contained within the volume. The cavity is then agitated.

Abstract translation: 本文件涉及一种将数量小的目标溶液与阵列混合的系统和方法。 例如，可以将一定量的目标溶液和不混溶液体的量引入到在内表面上具有阵列的空腔中。 目标溶液和不混溶液体的数量不能填满体积。 因此，体积内容纳有气体。 然后搅拌空腔。

公开(公告)号：US20060078899A1

公开(公告)日：2006-04-13

申请号：US10964505

申请日：2004-10-12

Applicant: Alicia Scheffer ,  Paige Anderson ,  Diane Ilsley ,  Michael Barrett ,  Bo Curry

Inventor： Alicia Scheffer ,  Paige Anderson ,  Diane Ilsley ,  Michael Barrett ,  Bo Curry

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6841 ,  C12Q2565/501 ,  C12Q2525/185

Abstract: Methods and compositions for producing a labeled population of nucleic acids for use in an array-based comparative genome hybridization (CGH) assay are provided. In general, the methods involve cleaving a genomic source to produce a sample containing nucleic acid fragments, and end-labeling the nucleic acid fragments to provide a population of nucleic acids having a terminal label. Kits and systems for use in practicing the subject methods are also provided.

Abstract translation: 提供了用于生产用于基于阵列的比较基因组杂交（CGH）测定中的标记的核酸群的方法和组合物。 通常，所述方法包括切割基因组来源以产生含有核酸片段的样品，并且终止标记核酸片段以提供具有末端标记的核酸群体。 还提供了用于实践主题方法的套件和系统。

公开(公告)号：US20060078898A1

公开(公告)日：2006-04-13

申请号：US10964504

申请日：2004-10-12

Applicant: Bo Curry ,  Paige Anderson

Inventor： Bo Curry ,  Paige Anderson

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6841 ,  C12Q2565/501 ,  C12Q2565/102 ,  C12Q2533/101

Abstract: Methods and compositions for performing array-based CGH assays are provided. In general, the methods involve evaluating copy number of a genomic region by evaluating binding of distinguishably labeled populations of nucleic acids to a first CGH array in which: a) the populations of nucleic acids are labeled by a labeled primer and/or b) binding is assessed relative to binding of dye-swapped populations of nucleic acids to a second CGH array. Kits and systems for use in practicing the subject methods are also provided.

Abstract translation: 提供了用于进行基于阵列的CGH测定法的方法和组合物。 通常，所述方法包括通过评估核酸的可区分标记的群体与第一CGH阵列的结合来评估基因组区域的拷贝数，其中：a）核酸群体被标记的引物和/或b）结合 相对于染色体交换的核酸群体与第二CGH阵列的结合来评估。 还提供了用于实践主题方法的套件和系统。

公开(公告)号：US06294366B1

公开(公告)日：2001-09-25

申请号：US09136574

申请日：1998-08-19

Applicant: Graham K. Farrington ,  Paige Anderson ,  Peter Bergquist ,  Roy Daniels ,  Moreland David Gibbs ,  Hugh Morgan ,  Diane P. Williams

Inventor： Graham K. Farrington ,  Paige Anderson ,  Peter Bergquist ,  Roy Daniels ,  Moreland David Gibbs ,  Hugh Morgan ,  Diane P. Williams

IPC: C12N942

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/13 ,  D06P5/15

Abstract: Alkalophilic and thermophilic cellulases having high stability to elevated temperatures and pH have been isolated from an organism of unknown species, which most closely resembles those in the Caldicellulosiruptor genus and which has been called by us, Tok7B.1, These cellulases have been cloned and expressed in a recombinant system, so that they can be produced in quantity. These are particularly useful in treating cellulosic materials including cotton-containing fabrics, as detergent additives, and in aqueous compositions. We also provide genomic DNA which can be used in recombinant expression vectors and expression systems to produce enhanced alkali and/or temperature stability properties in cellulases other than those specifically described.

Abstract translation: 已经从未知物种的生物体中分离出对升高的温度和pH具有高稳定性的嗜碱性和嗜热纤维素酶，其与Caldicellulosiruptor属中的那些非常相似，并且被我们称之为Tok7B.1。这些纤维素酶已被克隆和表达 在重组体系中，使得它们可以量产。 这些在处理包括含棉织物，作为洗涤剂添加剂的纤维素材料和水性组合物中特别有用。 我们还提供可用于重组表达载体和表达系统的基因组DNA，以在除了具体描述的纤维素酶之外的纤维素酶中产生增强的碱和/或温度稳定性。

公开(公告)号：US20060172316A1

公开(公告)日：2006-08-03

申请号：US11048152

申请日：2005-01-31

Applicant: Paige Anderson ,  Samar Lightfoot

Inventor： Paige Anderson ,  Samar Lightfoot

IPC: C12Q1/68 ,  G06F19/00 ,  C12P19/34

CPC classification number: C12Q1/6837 ,  C12Q2545/101

Abstract: A method of assessing the quality of an amplified nucleic acid sample is described. The method includes (a) separating the amplified nucleic acid sample to obtain a separation profile; (b) determining a first component of said separation profile corresponding to a first nucleic acid fraction; (c) determining a second component of said separation profile corresponding to a second nucleic acid fraction; and (d) assigning a quality value to the amplified nucleic acid sample based on the first component and the second component.

Abstract translation: 描述了评估扩增的核酸样品的质量的方法。 该方法包括（a）分离扩增的核酸样品以获得分离谱; （b）确定对应于第一核酸级分的所述分离谱的第一组分; （c）确定对应于第二核酸级分的所述分离谱的第二组分; 和（d）基于第一组分和第二组分将质量值分配给扩增的核酸样品。