公开(公告)号：US08951773B2

公开(公告)日：2015-02-10

申请号：US13699780

申请日：2011-05-25

申请人： Hiroaki Kato ,  Ko Yamashita ,  Yukio Fukushima ,  Ken Amano ,  Takashi Kaneko ,  Iwao Ueda ,  Nobuo Aoki ,  Kengo Suzuki ,  Ryo Arashida ,  Ryohei Nakano

发明人： Hiroaki Kato ,  Ko Yamashita ,  Yukio Fukushima ,  Ken Amano ,  Takashi Kaneko ,  Iwao Ueda ,  Nobuo Aoki ,  Kengo Suzuki ,  Ryo Arashida ,  Ryohei Nakano

IPC分类号： C12N1/00 ,  C12N1/12 ,  C12P7/64 ,  C10L1/02

CPC分类号： C12P7/64 ,  C10G2300/1014 ,  C10G2300/4043 ,  C10L1/02 ,  C11B1/10 ,  C11C3/00 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20 ,  Y02P30/446

摘要： The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S1) of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process (S2) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process (S3) of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process (S4) to reform the extracted oils and fats.

摘要翻译： 本发明提供了一种生物燃料的生产方法，其基于通过光合微生物通过光合作用将碳二
氧化碳转化为生物质并生产生物燃料的技术。 本发明的生物燃料的制造方法包括在光合微生物的细胞中储存油，脂肪和碳水化合物的培养液中培养光合微生物的培养方法（S1），将光合微生物的细胞中的油脂转化方法（S2） 储存在培养装置中培养的光合微生物的细胞中的碳水化合物用于油和脂肪，从光合微生物的细胞中提取油和脂肪的提取方法（S3）和重整方法（S4） 提取油脂。

公开(公告)号：US09045784B2

公开(公告)日：2015-06-02

申请号：US13811120

申请日：2011-07-13

申请人： Ryo Arashida ,  Sharbanee Mitra

发明人： Ryo Arashida ,  Sharbanee Mitra

IPC分类号： C12P7/64 ,  C12N1/12 ,  C12N15/82

CPC分类号： C12P7/64 ,  C12N1/12 ,  C12N15/8247 ,  C12P7/6463

摘要： Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester.The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.

摘要翻译： 本发明提供一种高度含有蜡酯的高丝虫素的制造方法，其特征在于，使用二氧化碳作为碳源，通过光合作用有氧培养微藻，然后在氮饥饿条件下进一步培养微藻，以增加每个细胞的亚甲酸蓄积量，随后 将微藻子球放置在厌氧条件下，从而可以生产高含有蜡酯的高丝虫; 和蜡酯的制造方法。 本发明涉及一种高度含有蜡酯的丝虫的生产方法。 该方法包括有氧培养微藻的第一步骤，在氮饥饿条件下进一步培养培养基的第二步骤，以及在厌氧条件下保持细胞的第三步骤。

公开(公告)号：US20130115666A1

公开(公告)日：2013-05-09

申请号：US13811120

申请日：2011-07-13

申请人： Ryo Arashida ,  Sharbanee Mitra

发明人： Ryo Arashida ,  Sharbanee Mitra

IPC分类号： C12P7/64 ,  C12N1/12

CPC分类号： C12P7/64 ,  C12N1/12 ,  C12N15/8247 ,  C12P7/6463

摘要： Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester.The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.

摘要翻译： 本发明提供一种高度含有蜡酯的高丝虫素的制造方法，其特征在于，使用二氧化碳作为碳源，通过光合作用有氧培养微藻，然后在氮饥饿条件下进一步培养微藻，以增加每个细胞的亚甲酸蓄积量，随后 将微藻子球放置在厌氧条件下，从而可以生产高含有蜡酯的高丝虫; 和蜡酯的制造方法。 本发明涉及一种高度含有蜡酯的丝虫的生产方法。 该方法包括有氧培养微藻的第一步骤，在氮饥饿条件下进一步培养培养基的第二步骤，以及在厌氧条件下保持细胞的第三步骤。

公开(公告)号：US20130071889A1

公开(公告)日：2013-03-21

申请号：US13699780

申请日：2011-05-25

申请人： Hiroaki Kato ,  Ko Yamashita ,  Yukio Fukushima ,  Ken Amano ,  Takashi Kaneko ,  Iwao Ueda ,  Nobuo Aoki ,  Kengo Suzuki ,  Ryo Arashida ,  Ryohei Nakano

发明人： Hiroaki Kato ,  Ko Yamashita ,  Yukio Fukushima ,  Ken Amano ,  Takashi Kaneko ,  Iwao Ueda ,  Nobuo Aoki ,  Kengo Suzuki ,  Ryo Arashida ,  Ryohei Nakano

IPC分类号： C12P7/64

CPC分类号： C12P7/64 ,  C10G2300/1014 ,  C10G2300/4043 ,  C10L1/02 ,  C11B1/10 ,  C11C3/00 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20 ,  Y02P30/446

摘要： The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S1) of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process (S2) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process (S3) of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process (S4) to reform the extracted oils and fats.

摘要翻译： 本发明提供了一种生物燃料的生产方法，其基于通过光合微生物通过光合作用将碳二
氧化碳转化为生物质并生产生物燃料的技术。 本发明的生物燃料的制造方法包括在光合微生物的细胞中储存油，脂肪和碳水化合物的培养液中培养光合微生物的培养方法（S1），将光合微生物的细胞中的油脂转化方法（S2） 储存在培养装置中培养的光合微生物的细胞中的碳水化合物用于油和脂肪，从光合微生物的细胞中提取油和脂肪的提取方法（S3）和重整方法（S4） 提取油脂。