公开(公告)号：US08735131B2

公开(公告)日：2014-05-27

申请号：US13062798

申请日：2009-08-12

申请人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

发明人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

IPC分类号： C12P21/00 ,  C12N15/00 ,  C12N9/78

CPC分类号： C12N9/80 ,  C12N9/48

摘要： An object is to provide a novel method for improving an enzyme capable of deamidating a protein. A mutant protein deamidase is designed by the following steps: (A) identifying one or more amino acid in an amino acid sequence for a protein deamidase which corresponds to the amino acid at position 35, 38 to 43, 45, 46, 49, 79 to 84, 103 to 106, 117, 142, 143, 146, 166, or 185 in the amino acid sequence set forth in SEQ ID NO: 2; and (B) constructing a mutant amino acid sequence of the protein deamidase by substituting the one or more amino acid identified in step (A) with another amino acid or other amino acids or by deleting the one or more amino acid identified in step (A).

摘要翻译： 目的是提供一种改善能够使蛋白质脱酰胺化的酶的新方法。 通过以下步骤设计突变型蛋白质脱酰胺酶：（A）鉴定氨基酸序列中一个或多个对应于第35,38,43,45,46,49,79位的氨基酸的蛋白质脱酰胺酶的氨基酸 至SEQ ID NO：2所示氨基酸序列中的84,103至106,117,142,143,146,166或185; 和（B）通过用步骤（A）中鉴定的一个或多个氨基酸用另一个氨基酸或其他氨基酸取代蛋白质脱酰胺酶的突变氨基酸序列，或通过删除步骤（A）中鉴定的一个或多个氨基酸 ）。

公开(公告)号：US09153848B2

公开(公告)日：2015-10-06

申请号：US13274715

申请日：2011-10-17

申请人： Ryoichi Nakashima ,  Ryota Hashizume ,  Jiro Moriya ,  Yukio Tsuchiya ,  Junya Yamada ,  Takashi Mutou

发明人： Ryoichi Nakashima ,  Ryota Hashizume ,  Jiro Moriya ,  Yukio Tsuchiya ,  Junya Yamada ,  Takashi Mutou

IPC分类号： G01R31/36 ,  H01M10/48 ,  H01M10/42 ,  H01M10/0525

CPC分类号： H01M10/48 ,  G01R31/361 ,  G01R31/3682 ,  H01M10/0525 ,  H01M10/425 ,  H01M10/488

摘要： An electronic device includes a battery capacity computing unit configured to compute a current full-charge capacity of a currently inserted battery apparatus having secondary cells. The battery capacity computing unit acquires at least information on a full-charge capacity of the battery apparatus in a brand-new state and information on the battery charge/discharge cycle count from the battery apparatus, retains a correction coefficient used when computing the current full-charge capacity of the battery apparatus, and calculates the current full-charge capacity of the battery apparatus using the information on the full-charge capacity of the battery apparatus in a brand-new state, the information on the battery charge/discharge cycle count, and the correction coefficient.

摘要翻译： 电子设备包括电池容量计算单元，其被配置为计算当前插入的具有二次电池的电池装置的当前满充电容量。 电池容量计算单元至少获取关于全新状态的电池装置的满充电容量的信息和来自电池装置的电池充电/放电周期计数的信息，保留当计算当前充满电时使用的校正系数 电池装置的充电容量，并且使用关于全新状态的电池装置的全充电容量的信息来计算电池装置的当前满充电容量，关于电池充电/放电周期数的信息 ，和校正系数。

公开(公告)号：US20110165605A1

公开(公告)日：2011-07-07

申请号：US13062798

申请日：2009-08-12

申请人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

发明人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

IPC分类号： C12Q1/34 ,  C12N9/00 ,  C12N9/80 ,  C12N9/78 ,  C07H21/04 ,  C12N1/00

CPC分类号： C12N9/80 ,  C12N9/48

摘要： An object is to provide a novel method of improving an enzyme capable of deamidating a protein. A mutant enzyme is designed by the following steps: (1) specifying one or more amino acids selected from the following group, namely, consisting of an amino acid corresponding to the amino acid at position 35, an amino acid corresponding to the amino acid at position 38, an amino acid corresponding to the amino acid at position 39, an amino acid corresponding to the amino acid at position 40, an amino acid corresponding to the amino acid at position 41, an amino acid corresponding to the amino acid at position 42, an amino acid corresponding to the amino acid at position 43, an amino acid corresponding to the amino acid at position 45, an amino acid corresponding to the amino acid at position 46, an amino acid corresponding to the amino acid at position 49, an amino acid corresponding to the amino acid at position 79, an amino acid corresponding to the amino acid at position 80, an amino acid corresponding to the amino acid at position 81, an amino acid corresponding to the amino acid at position 82, an amino acid corresponding to the amino acid at position 83, an amino acid corresponding to the amino acid at position 84, an amino acid corresponding to the amino acid at position 103, an amino acid corresponding to the amino acid at position 104, an amino acid corresponding to the amino acid at position 105, an amino acid corresponding to the amino acid at position 106, an amino acid corresponding to the amino acid at position 117, an amino acid corresponding to the amino acid at position 142, an amino acid corresponding to the amino acid at position 143, an amino acid corresponding to the amino acid at position 146, an amino acid corresponding to the amino acid at position 166, and an amino acid corresponding to the amino acid at position 185 in an amino acid sequence set forth in SEQ ID NO: 2, in a protein deamidase (an enzyme to be mutated); and (2) constructing an amino acid sequence having substitution of the amino acid(s) specified in the step (1) by another amino acid(s) or having deletion of the amino acid(s) specified in the step (1) using the amino acid sequence for an enzyme to be mutated as a base sequence.

公开(公告)号：US20120150465A1

公开(公告)日：2012-06-14

申请号：US13274715

申请日：2011-10-17

申请人： Ryoichi NAKASHIMA ,  Ryota Hashizume ,  Jiro Moriya ,  Yukio Tsuchiya ,  Junya Yamada ,  Takashi Mutou

发明人： Ryoichi NAKASHIMA ,  Ryota Hashizume ,  Jiro Moriya ,  Yukio Tsuchiya ,  Junya Yamada ,  Takashi Mutou

IPC分类号： G01R31/36

CPC分类号： H01M10/48 ,  G01R31/361 ,  G01R31/3682 ,  H01M10/0525 ,  H01M10/425 ,  H01M10/488

摘要： An electronic device includes a battery capacity computing unit configured to compute the current full-charge capacity of a currently inserted battery apparatus having secondary cells. The battery capacity computing unit acquires at least information on the full-charge capacity of the battery apparatus in a brand-new state and information on the battery charge/discharge cycle count from the battery apparatus, retains a correction coefficient used when computing the current full-charge capacity of the battery apparatus, and calculates the current full-charge capacity of the battery apparatus using the information on the full-charge capacity of the battery apparatus in a brand-new state, the information on the battery charge/discharge cycle count, and the correction coefficient.

摘要翻译： 电子设备包括电池容量计算单元，其配置为计算当前插入的具有二次电池的电池装置的当前满充电容量。 电池容量计算单元至少获取关于全新状态的电池装置的满充电容量的信息和来自电池装置的电池充放电循环数的信息，保持当计算当前充满电时使用的校正系数 电池装置的充电容量，并且使用关于全新状态的电池装置的全充电容量的信息来计算电池装置的当前满充电容量，关于电池充电/放电周期数的信息 ，和校正系数。