公开(公告)号：US20130122580A1

公开(公告)日：2013-05-16

申请号：US13521173

申请日：2011-01-07

申请人： Ryouhei Tsukada ,  Kanehisa Yokoyama

发明人： Ryouhei Tsukada ,  Kanehisa Yokoyama

IPC分类号： C12M1/00

CPC分类号： C12M31/08 ,  C08J7/047 ,  C08J7/18 ,  C08J2325/06 ,  C08J2429/04 ,  C12M23/12 ,  C12M23/20 ,  C12M41/06 ,  G01N21/6486 ,  G01N33/5005

摘要： The present invention provides a culture vessel for forming an aggregated cell mass which has at least two wells, wherein the wells are made from a light-shielding member and an inner surface of the wells is subjected to treatment to impart cellular non-adhesiveness.

公开(公告)号：US20140011269A1

公开(公告)日：2014-01-09

申请号：US14006367

申请日：2012-03-28

申请人： Takeshi Sakura ,  Ryouhei Tsukada

发明人： Takeshi Sakura ,  Ryouhei Tsukada

IPC分类号： C12N5/073

CPC分类号： C12N5/0603 ,  C12M21/06 ,  C12M23/12 ,  C12M23/20

摘要： The present invention provides a culture vessel for forming an embryoid body that makes it possible to form an embryoid body of higher quality, more efficiently, at a higher level. The present invention relates to a culture vessel for forming an embryoid body that has two or more wells, in which the wells have a bottom having a cross section that looks like approximately a U shape when being observed in a vertical direction and an opening having an approximately circular shape, at least a curved portion of the inner surface of the bottom has low cell adhesive properties, a curvature radius (R′) of the inner surface of the bottom is from 1.0 mm to 3.5 mm, and the low cell adhesion surface is formed by being performed low-or-non cell adhesion treatment with a water-soluble resin.

摘要翻译： 本发明提供一种用于形成胚状体的培养容器，其使得可以在更高水平上更有效地形成更高质量的胚状体。 本发明涉及一种用于形成具有两个或更多个孔的胚状体的培养容器，其中所述孔具有在垂直方向上观察时具有大致U形的截面的底部，以及具有 大致圆形，底部内表面的至少弯曲部分具有低的电池粘合性能，底部内表面的曲率半径（R'）为1.0mm至3.5mm，低电池粘附表面 是通过用水溶性树脂进行低或非细胞粘附处理而形成的。