利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

14 条记录 (耗时 0.017 秒)

    Method and kit for detection/quantification of target RNA
    2.
    发明授权
    Method and kit for detection/quantification of target RNA 有权
    靶RNA的检测/定量的方法和试剂盒

    公开(公告)号：US08435742B2

    公开(公告)日：2013-05-07

    申请号：US12739978

    申请日：2008-11-04

    申请人： Mutsunori Shirai ,  Hajime Fukunaga ,  Kazuhiko Fujiwara ,  Kanehisa Yokoyama ,  Kentaro Fujimoto

    发明人： Mutsunori Shirai ,  Hajime Fukunaga ,  Kazuhiko Fujiwara ,  Kanehisa Yokoyama ,  Kentaro Fujimoto

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/00 ,  C07H21/04

    CPC分类号： C12Q1/6809 ,  C12Q2565/537 ,  C12Q2525/143 ,  C12Q2521/107

    摘要： [Object] It is to provide a method and a kit capable of detecting or quantifying a target RNA simply and rapidly from trace amounts of RNA in a sample, in a case such as when one or more kinds of pathogenic microorganisms are to be detected or quantified.[Solving Means] The method comprises the steps of 1) synthesizing cDNA from a sample containing the target RNA using a liquid-phase primer having a promoter sequence and a reverse transcriptase to obtain a cDNA-RNA complex, 2) degrading the RNA of the complex, 3) synthesizing a double-stranded DNA via the cDNA obtained in the step 2) and the solid-phase primer, 4) synthesizing RNA from the double-stranded DNA, 5) synthesizing cDNA via the RNA obtained in the step 4) and the solid-phase primer to obtain a cDNA-RNA complex, 6) degrading the RNA of the complex obtained in step 5), 7) synthesizing a double-stranded DNA via the cDNA obtained in the step 6) and the liquid-phase primer, and 8) quantifying the double-stranded DNAs obtained in the steps 3) and 7). These steps can be performed in a single reaction solution.

    摘要翻译： 本发明提供一种方法和试剂盒，其能够在样品中的痕量RNA中简单快速地检测或定量靶RNA，例如当检测到一种或多种病原微生物或 量化 [解决方法]该方法包括以下步骤：1）使用具有启动子序列和逆转录酶的液相引物合成来自含有靶RNA的样品的cDNA，得到cDNA-RNA复合物，2）降解 复合物，3）通过步骤2）和固相引物获得的cDNA合成双链DNA，4）从双链DNA合成RNA，5）通过步骤4）得到的RNA合成cDNA， 和固相引物以获得cDNA-RNA复合物，6）降解步骤5）中获得的复合物的RNA，7）通过步骤6）中得到的cDNA合成双链DNA和液相 引物，和8）定量在步骤3）和7）中获得的双链DNA。 这些步骤可以在单个反应溶液中进行。

    Method of synthesizing cdna and method of synthesizing rna chains, and nucleotide-immobilized carrier
    4.
    发明申请
    Method of synthesizing cdna and method of synthesizing rna chains, and nucleotide-immobilized carrier 有权
    合成cdna的方法和合成rna链的方法以及核苷酸固定载体

    公开(公告)号：US20100016566A1

    公开(公告)日：2010-01-21

    申请号：US11990641

    申请日：2006-08-09

    申请人： Kenji Kinoshita ,  Toru Yakabe ,  Kentaro Fujimoto ,  Kanehisa Yokoyama ,  Kazuhiko Fujiwara

    发明人： Kenji Kinoshita ,  Toru Yakabe ,  Kentaro Fujimoto ,  Kanehisa Yokoyama ,  Kazuhiko Fujiwara

    IPC分类号： C07H21/04 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12Q1/686 ,  C12Q2521/107 ,  C12Q2565/537

    摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.

    摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。

    Method of detecting gene
    5.
    发明授权
    Method of detecting gene 失效
    检测基因的方法

    公开(公告)号：US07968290B2

    公开(公告)日：2011-06-28

    申请号：US11920559

    申请日：2006-05-16

    申请人： Kenji Kinoshita ,  Kazuhiko Fujiwara ,  Kanehisa Yokoyama ,  Kentaro Fujimoto ,  Toru Yakabe

    发明人： Kenji Kinoshita ,  Kazuhiko Fujiwara ,  Kanehisa Yokoyama ,  Kentaro Fujimoto ,  Toru Yakabe

    IPC分类号： C12Q1/68 ,  C12Q1/26 ,  C12Q1/28 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1096 ,  C12Q1/6834 ,  C12Q2565/537 ,  C12Q2527/107

    摘要： A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration.

    摘要翻译： 一种检测基因的方法，包括将DNA延伸引物固定在具有表面的不溶性载体上，所述不溶性载体具有含有具有磷酸胆碱基团的第一单元的聚合物物质和具有吸电子取代基的羧酸衍生基团的第二单元 与羰基结合; 用引物退火模板DNA片段或RNA片段以进行DNA延伸，以便在引入酶的同时延长DNA引物，从而通过其酶作用使着色试剂着色; 并基于着色程度来判断该基因的DNA片段或RNA片段是否存在。

    RNA Detection Method
    6.
    发明申请
    RNA Detection Method 有权
    RNA检测方法

    公开(公告)号：US20090137406A1

    公开(公告)日：2009-05-28

    申请号：US12227904

    申请日：2007-06-01

    申请人： Kenji Kinoshita ,  Kanehisa Yokoyama ,  Kentaro Fujimoto ,  Toru Yakabe ,  Shin Saito ,  Kazuhiko Fujiwara

    发明人： Kenji Kinoshita ,  Kanehisa Yokoyama ,  Kentaro Fujimoto ,  Toru Yakabe ,  Shin Saito ,  Kazuhiko Fujiwara

    IPC分类号： C40B30/00 ,  C12Q1/68

    CPC分类号： C12Q1/6837

    摘要： The present invention provides an RNA detection method detecting, from a reaction system containing a target sample, a target RNA chain originated from the target sample, using a surface having on the surface thereof a polymer substance which contains a first unit having a group derived from a phosphate ester composing the hydrophilic portion of a phospholipid and a second unit having a carboxylic acid derivative group composed of an electron-attractive substitutional group bound to a carbonyl group, while being provided with at least one reaction space, the reaction space having an immobilized nucleic acid primer immobilized therein.

    摘要翻译： 本发明提供一种RNA检测方法，其从含有目标样品的反应体系中检测源自目标样品的靶RNA链，其表面上具有含有第一单元的表面，该第一单元含有来自 构成磷脂的亲水部分的磷酸酯和具有由与羰基结合的吸电子取代基组成的羧酸衍生物基团的第二单元，同时具有至少一个反应空间，所述反应空间具有固定化的 固定在其中的核酸引物。

    Biochip
    7.
    发明申请
    Biochip 审中-公开
    生物芯片

    公开(公告)号：US20150005180A9

    公开(公告)日：2015-01-01

    申请号：US10572332

    申请日：2004-09-17

    申请人： Kazuhiko Ishihara ,  Sohei Funaoka ,  Kanehisa Yokoyama

    发明人： Kazuhiko Ishihara ,  Sohei Funaoka ,  Kanehisa Yokoyama

    IPC分类号： C40B30/04 ,  C40B60/12 ,  C40B40/08 ,  C40B40/12 ,  C40B50/18 ,  C40B99/00 ,  C40B40/10

    CPC分类号： C12Q1/003

    摘要： A biochip is provided that suppresses nonspecific adsorption or bonding of a detection target substance without coating with an adsorption inhibitor and that has excellent detection sensitivity. The constitution is such that it has a macromolecular substance containing a phosphorylcholine group and an active ester group on a substrate surface of a biochip substrate.

    摘要翻译： 提供一种生物芯片，其抑制检测对象物质的非特异性吸附或结合而不用吸附抑制剂涂覆并且具有优异的检测灵敏度。 其结构是在生物芯片基板的基板表面上具有含有磷酰胆碱基和活性酯基的大分子物质。

    Biochip
    8.
    发明申请
    Biochip 审中-公开
    生物芯片

    公开(公告)号：US20100222226A1

    公开(公告)日：2010-09-02

    申请号：US10572332

    申请日：2004-09-17

    申请人： Kazuhiko Ishihara ,  Sohei Funaoka ,  Kanehisa Yokoyama

    发明人： Kazuhiko Ishihara ,  Sohei Funaoka ,  Kanehisa Yokoyama

    IPC分类号： C40B30/04 ,  C40B99/00 ,  C40B60/12 ,  C40B40/08 ,  C40B40/10 ,  C40B40/12 ,  C40B50/18

    CPC分类号： C12Q1/003

    摘要： To provide an electronic component mounting system and an electronic component mounting method which can prevent a mounting failure due to positional error in a height direction of a substrate and ensure mounting quality. The electronic component mounting system includes a plurality of electronic component mounting devices connected to one another and mounts an electronic component on a substrate to manufacture a mounting substrate. A print test device for testing the substrate after solder printing measures a height position of a height measurement point set on the upper surface of the substrate 4 by a height measuring machine 22 and outputs a measurement result as substrate height data. In a component placing step using an electronic component placing device, a control parameter for controlling a component placing operation of the placing head 32 is updated. Accordingly, it is possible to correct a variation of the height position of an individual substrate and to prevent a mounting failure due to positional error in the height direction of the substrate.

    摘要翻译： 提供一种能够防止由于基板的高度方向上的位置误差引起的安装故障并确保安装质量的电子部件安装系统和电子部件安装方法。 电子部件安装系统包括彼此连接的多个电子部件安装装置，并将电子部件安装在基板上以制造安装基板。 用于在焊接印刷之后测试基板的印刷测试装置通过高度测量机22测量设置在基板4的上表面上的高度测量点的高度位置，并输出测量结果作为基板高度数据。 在使用电子部件放置装置的部件放置步骤中，更新用于控制放置头32的部件放置操作的控制参数。 因此，可以校正单个基板的高度位置的变化，并且可以防止由于基板的高度方向上的位置误差引起的安装故障。

    Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier
    10.
    发明授权
    Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier 有权
    合成cDNA的方法和合成RNA链的方法以及核苷酸固定载体

    公开(公告)号：US08822670B2

    公开(公告)日：2014-09-02

    申请号：US11990641

    申请日：2006-08-09

    申请人： Kenji Kinoshita ,  Toru Yakabe ,  Kentaro Fujimoto ,  Kanehisa Yokoyama ,  Kazuhiko Fujiwara

    发明人： Kenji Kinoshita ,  Toru Yakabe ,  Kentaro Fujimoto ,  Kanehisa Yokoyama ,  Kazuhiko Fujiwara

    IPC分类号： C07H21/00

    CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12Q1/686 ,  C12Q2521/107 ,  C12Q2565/537

    摘要： A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing the hydrophilic portion of phospholipid, and a second unit having a group derived from carboxylic acid having an electron-attractive substituent bound to a carbonyl group, which includes immobilizing a polynucleotide for DNA elongation; bringing a solution containing an RNA fragment, nucleotide monomers, and a reverse transcriptase or an enzyme having polymerase activity into contact with the surface of the insoluble carrier; and allowing the polynucleotide for DNA elongation immobilized on the surface of the carrier to elongate using the RNA fragment contained in the solution as a template, to thereby form a single-strand cDNA.

    摘要翻译： 一种使用不溶性载体合成cDNA链的方法，所述不溶性载体在其表面上具有含有源自构成磷脂的亲水部分的磷酸酯的基团的第一单元的聚合物物质和具有衍生自羧酸的基团的第二单元， 与羰基结合的电子吸引取代基，其包括固定多核苷酸用于DNA延伸; 将含有RNA片段，核苷酸单体和逆转录酶或具有聚合酶活性的酶的溶液与不溶性载体的表面接触; 并且使用溶液中包含的RNA片段作为模板，使固定在载体表面上的DNA延伸的多核苷酸伸长，从而形成单链cDNA。