公开(公告)号：US10073085B2

公开(公告)日：2018-09-11

申请号：US14008348

申请日：2012-03-29

申请人： Mitsuru Akashi ,  Michiya Matsusaki ,  Takeshi Sakura ,  Koji Hashimoto ,  Yuji Shirakata ,  Satoshi Hirakawa

发明人： Mitsuru Akashi ,  Michiya Matsusaki ,  Takeshi Sakura ,  Koji Hashimoto ,  Yuji Shirakata ,  Satoshi Hirakawa

IPC分类号： G01N33/50 ,  C12N5/071

CPC分类号： G01N33/5088 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1192 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2503/06 ,  C12N2533/52 ,  C12N2533/54

摘要： Provided is a novel method capable of producing an artificial skin model. A method for producing an artificial skin model includes: providing coated cells (1), each of which is obtained by covering the surface of a cell (3) with a coating film (2) containing an extracellular matrix component; forming a dermis tissue layer (7), in which the coated cells (1) are laminated, by culturing the coated cells (1); and forming an epidermis layer (12) by arranging epidermis cells (8) on the dermis tissue layer (7).

公开(公告)号：US20140024010A1

公开(公告)日：2014-01-23

申请号：US14008348

申请日：2012-03-29

申请人： Mitsuru Akashi ,  Michiya Matsusaki ,  Takeshi Sakura ,  Koji Hashimoto ,  Yuji Shirakata ,  Satoshi Hirakawa

发明人： Mitsuru Akashi ,  Michiya Matsusaki ,  Takeshi Sakura ,  Koji Hashimoto ,  Yuji Shirakata ,  Satoshi Hirakawa

IPC分类号： G01N33/50

CPC分类号： G01N33/5088 ,  C12N5/0698 ,  C12N2502/094 ,  C12N2502/1192 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2503/06 ,  C12N2533/52 ,  C12N2533/54

摘要： Provided is a novel method capable of producing an artificial skin model. A method for producing an artificial skin model includes: providing coated cells (1), each of which is obtained by covering the surface of a cell (3) with a coating film (2) containing an extracellular matrix component; forming a dermis tissue layer (7), in which the coated cells (1) are laminated, by culturing the coated cells (1); and forming an epidermis layer (12) by arranging epidermis cells (8) on the dermis tissue layer (7).

摘要翻译： 提供了一种能够生产人造皮肤模型的新方法。 一种生产人造皮肤模型的方法包括：提供涂覆细胞（1），每个涂覆细胞通过用包含细胞外基质组分的涂膜（2）覆盖细胞（3）的表面而获得; 通过培养所述被覆细胞（1），形成层叠所述被覆细胞（1）的真皮组织层（7）。 以及通过在真皮组织层（7）上布置表皮细胞（8）形成表皮层（12）。

公开(公告)号：US5475234A

公开(公告)日：1995-12-12

申请号：US280811

申请日：1994-07-26

申请人： Kexin Xu ,  Yutaka Yamasaki ,  Harumi Uenoyama ,  Takeshi Sakura

发明人： Kexin Xu ,  Yutaka Yamasaki ,  Harumi Uenoyama ,  Takeshi Sakura

IPC分类号： G01N15/06 ,  A61B5/00 ,  G01N21/27 ,  G01N21/49 ,  G01N21/85

CPC分类号： A61B5/1455 ,  A61B5/14532 ,  G01N21/49 ,  G01N2021/1789 ,  G01N2021/1791 ,  G01N2021/4797 ,  G01N21/359

摘要： An apparatus and method for measuring component concentrations which enables accurate measurement of concentrations of component within an object in a non-destructive, non-invasive, and bloodless procedure. The apparatus comprises a light irradiator 1 for projecting pulse laser of different wavelengths toward an object 12 by means of a variable wavelength laser generating unit 11, a photodetector 2 for performing a time resolved measurement of the quantity of transmitted light from the object which is subject to changes with lapse of time after the generation of the pulse laser, and an arithmetic unit 3 for calculating concentrations of components in the object 12 and outputting the calculation result such that the relationship between time lapse and measured quantity of light is converted into a relationship between an optical path length representing the distance of light travel within the object 12 and a change in the measured quantity of light relative to a change in unit concentration so that the optical path length and the quantity of light which is measured when the change in the quantity of light is at a peak are stored in memory for each wavelength, the quantity of light being used as a basis for the calculation.

摘要翻译： 一种用于测量组分浓度的装置和方法，其能够在非破坏性，非侵入性和无血液过程中精确测量物体内的组分的浓度。 该装置包括用于通过可变波长激光产生单元11将不同波长的脉冲激光投射到物体12的光照射器1，用于对来自被摄体的物体的透射光进行时间分辨测量的光电检测器2 在产生脉冲激光之后经过时间的变化，以及用于计算对象12中的分量的浓度的运算单元3，并输出计算结果，使得时间流逝和测量光量之间的关系被转换为关系 在表示物体12内的光行进距离的光路长度与测量的光量相对于单位浓度的变化的变化之间，使得光路长度和当光线长度变化时所测量的光量 光量处于峰值时，存储在每个波长的存储器中，光量为我们 作为计算的基础。

公开(公告)号：US5615009A

公开(公告)日：1997-03-25

申请号：US630974

申请日：1996-04-12

申请人： Takeshi Sakura ,  Yutaka Yamasaki ,  Hiroko Kubo ,  Kexin Xu ,  Motonobu Shiomi

发明人： Takeshi Sakura ,  Yutaka Yamasaki ,  Hiroko Kubo ,  Kexin Xu ,  Motonobu Shiomi

IPC分类号： G01J3/28 ,  G01N21/27 ,  G01J3/42

CPC分类号： G01N21/274

摘要： A method of quantitative analysis of a specific component of an object to be measured comprises steps of measuring an energy spectrum of light transmitted through or reflected from the object, dividing the energy spectrum into a plurality of wavelength domains, thereby obtaining a plurality of partial energy spectra, normalizing the plurality of partial energy spectra within each wavelength domain using an energy measured at a predetermined wavelength contained in each wavelength domain, and performing the quantative analysis by multivariate analysis using the plurality of partial energy spectra having been normalized.

摘要翻译： 对被测量物体的特定成分进行定量分析的方法包括以下步骤：测量透过物体或通过物体反射的光的能谱，将能谱分成多个波长域，从而获得多个部分能量 光谱，使用在每个波长域中包含的预定波长测量的能量对每个波长域内的多个部分能谱进行标准化，以及使用已经被归一化的多个部分能谱通过多变量分析进行定量分析。

公开(公告)号：US5602647A

公开(公告)日：1997-02-11

申请号：US562785

申请日：1995-11-27

申请人： Kexin Xu ,  Yutaka Yamasaki ,  Harumi Uenoyama ,  Takeshi Sakura

发明人： Kexin Xu ,  Yutaka Yamasaki ,  Harumi Uenoyama ,  Takeshi Sakura

IPC分类号： G01N21/27 ,  G01J3/42 ,  G01N21/03 ,  G01N21/31 ,  G01N21/39 ,  G01N21/59 ,  G01N21/00

CPC分类号： G01N21/0303 ,  G01N21/31 ,  G01J3/42 ,  G01N2021/3129 ,  G01N21/39 ,  G01N21/59 ,  G01N2201/066 ,  G01N2201/0668

摘要： An apparatus and method for optically measuring concentrations of components allow enhancement in measurement accuracy of concentration. The apparatus includes a cell, a light irradiator, a photodetector, and an arithmetic unit. The cell presents different optical path lengths at different locations and is to contain a sample therein. The light irradiator, which includes a variable-wavelength laser generator and a measuring system composed of convex lenses, outputs a collimated, enlarged laser beam, and makes the laser beam incident upon the cell. The photodetector comprises a multiplicity of photodetectors arranged in parallel to the surface of the cell, so that it can detect intensity of rays of transmitted light that have traveled over different optical path lengths at positions of an equal distance from the cell. The arithmetic unit, receiving a signal from the individual photodetectors, calculates concentrations of components in the sample based on optimum optical path lengths for different wavelengths and values of transmitted light at positions of the optimum optical path lengths, and further outputs calculation results.

摘要翻译： 用于光学测量组分浓度的装置和方法允许增强浓度的测量精度。 该装置包括单元，光照射器，光电检测器和运算单元。 细胞在不同位置呈现不同的光程长度，并且其中包含样品。 包括可变波长激光发生器和由凸透镜组成的测量系统的光照射器输出准直的，放大的激光束，并使激光束入射到电池上。 光电检测器包括与电池表面平行设置的多个光电检测器，使得其可以检测在距离电池相等距离的位置处已经在不同光程长度上行进的透射光的光强。 运算单元接收来自各个光电检测器的信号，根据最佳光程长度的不同波长的最佳光路长度和最佳光路长度位置处的透射光值，计算样品中的成分浓度，并进一步输出计算结果。

公开(公告)号：US20140011269A1

公开(公告)日：2014-01-09

申请号：US14006367

申请日：2012-03-28

申请人： Takeshi Sakura ,  Ryouhei Tsukada

发明人： Takeshi Sakura ,  Ryouhei Tsukada

IPC分类号： C12N5/073

CPC分类号： C12N5/0603 ,  C12M21/06 ,  C12M23/12 ,  C12M23/20

摘要： The present invention provides a culture vessel for forming an embryoid body that makes it possible to form an embryoid body of higher quality, more efficiently, at a higher level. The present invention relates to a culture vessel for forming an embryoid body that has two or more wells, in which the wells have a bottom having a cross section that looks like approximately a U shape when being observed in a vertical direction and an opening having an approximately circular shape, at least a curved portion of the inner surface of the bottom has low cell adhesive properties, a curvature radius (R′) of the inner surface of the bottom is from 1.0 mm to 3.5 mm, and the low cell adhesion surface is formed by being performed low-or-non cell adhesion treatment with a water-soluble resin.

摘要翻译： 本发明提供一种用于形成胚状体的培养容器，其使得可以在更高水平上更有效地形成更高质量的胚状体。 本发明涉及一种用于形成具有两个或更多个孔的胚状体的培养容器，其中所述孔具有在垂直方向上观察时具有大致U形的截面的底部，以及具有 大致圆形，底部内表面的至少弯曲部分具有低的电池粘合性能，底部内表面的曲率半径（R'）为1.0mm至3.5mm，低电池粘附表面 是通过用水溶性树脂进行低或非细胞粘附处理而形成的。

公开(公告)号：US06903335B1

公开(公告)日：2005-06-07

申请号：US09701962

申请日：1999-06-04

申请人： Toshihisa Inoue ,  Takeshi Sakura ,  Norimasa Noda

发明人： Toshihisa Inoue ,  Takeshi Sakura ,  Norimasa Noda

IPC分类号： G01N27/401 ,  G01N33/487 ,  G09B19/00 ,  G09B21/00 ,  G01N27/30

CPC分类号： G09B19/0038 ,  G01N27/401 ,  G09B21/001 ,  G09B21/003

摘要： The ion activity-measuring device of the present invention is provided with a hydrophobic bridge of which portion contacting with a liquid reservoir is hydrophilic. The hydrophobic bridge is made of, for example, at least one selected from the group consisting of polyester, nylon, polypropylene, rayon and polyethylene, and is produced by treating a portion contacting with the liquid reservoir with a spreading accelerator. The spreading accelerator is at least one selected from the group consisting of a surfactant and a hydrophilic polymer. There is also provided a method for producing the above ion activity-measuring device, comprising embedding nonwoven fabric in the cover plate to bond the nonwoven fabric to the cover plate.

摘要翻译： 本发明的离子活性测定装置设置有与液体储存部接触的部分是亲水性的疏水性桥接体。 疏水桥由例如由聚酯，尼龙，聚丙烯，人造纤维和聚乙烯组成的组中的至少一种制成，并且通过用扩散促进剂处理与液体储存器接触的部分来制造。 铺展促进剂是选自表面活性剂和亲水性聚合物中的至少一种。 还提供了一种用于制造上述离子活度测量装置的方法，包括将无纺布嵌入到盖板中以将无纺布粘合到盖板上。

公开(公告)号：US5772606A

公开(公告)日：1998-06-30

申请号：US397953

申请日：1995-03-03

申请人： Emi Ashibe ,  Takeshi Sakura ,  Harumi Uenoyama ,  Xu Kexin ,  Hiroko Kubo

发明人： Emi Ashibe ,  Takeshi Sakura ,  Harumi Uenoyama ,  Xu Kexin ,  Hiroko Kubo

IPC分类号： G01N21/35 ,  G01N33/483 ,  G01N33/493 ,  A61B5/00

CPC分类号： G01N33/493 ,  G01N21/359

摘要： A urinal is provided on its forward end with a cell, which internally communicates with the urinal to outwardly project from the same. A measuring part comprises a light source part for applying a measuring beam of the visible or near infrared wavelength region to the cell which is set in a cell setting part and a light receiving part for receiving and detecting the measuring beam transmitted through the cell, and measures absorbances of uric components to be measured at wavelengths selected therefor respectively. A sensor part is adapted to detect that the cell is set in the cell setting part, so that the measuring part starts a measuring operation on the basis of a signal indicating that the sensor detects the cell. It is possible to carry out a urine test on a person who lies on bed.

摘要翻译： 在其前端设有一个小便器，一个细胞，其内部与小便器通信以向外突出。 测量部分包括用于将可见光或近红外波长区域的测量光束施加到设置在单元设置部分中的单元的光源部分和用于接收和检测透过单元的测量光束的光接收部分，以及 测量在分别选择的波长处测量的尿液成分的吸光度。 传感器部分适于检测单元设置在单元设置部分中，使得测量部件基于表示传感器检测到单元的信号开始测量操作。 可以对躺在床上的人进行尿检。