公开(公告)号：US20120015399A1

公开(公告)日：2012-01-19

申请号：US13147042

申请日：2010-02-04

申请人： Koji Yamada ,  Yutaka Yamagishi ,  Tokiyoshi Ayabe ,  Sang-Hyun Son ,  Maiko Aoyagi ,  Toshio Taira

发明人： Koji Yamada ,  Yutaka Yamagishi ,  Tokiyoshi Ayabe ,  Sang-Hyun Son ,  Maiko Aoyagi ,  Toshio Taira

IPC分类号： G01N1/30 ,  C12N5/071 ,  C07D409/04

CPC分类号： C07D409/04 ,  C07D333/20 ,  C09B57/00 ,  C09K9/02 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1029 ,  C09K2211/1044 ,  C09K2211/1092

摘要： The present invention presents a novel fluorescent solvatochromic dye that (1) has an ionic terminal that makes it easier to use in a hydrophilic surface or in polar solvents, (2) can be efficiently excited by commonly used Argon lasers (488 nm), (3) shifts the wavelength of emitted light according to the change of polarity, and (4) can effectively stain living tissues such as cells and the like.A pyridinium group was introduced to the electron attracting group of the neutral fluorescent solvatochromic dye (Japanese Patent Application Public Disclosure No. 20008-291210 A) displaying an excellent emission wavelength response and synthesized a fluorescent solvatochromic dye. Then it was found that the fluorescence wavelength of the fluorescent solvatochromic dye changed extensively when the polarity changed on a hydrophilic surface and that the fluorescent solvatochromic dye, when altered to a cationic form, stained cell membranes and could be used to observe the behavior thereof.

摘要翻译： 本发明提供了一种新的荧光溶剂化染料，其具有使其易于在亲水性表面或极性溶剂中使用的离子性末端，（2）可以通过常用的氩激光（488nm），（ 3）根据极性的变化使发射光的波长发生变化，（4）能够有效地染色细胞等活体组织。 将吡啶鎓基引入到显示出优异的发射波长响应的中性荧光溶剂化染料（日本专利申请公开No.20008-291210A）的吸电子基团中，并合成荧光溶剂化染料。 然后发现当极性在亲水表面上变化时，荧光溶剂化染料染料的荧光波长变化很大，而当改变为阳离子形式时，荧光溶剂化染料染色细胞膜并可用于观察其行为。