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    Map-Based Genome Mining Method for Identifying Regulatory Loci Controlling the Level of Gene Transcripts and Products
    2.
    发明申请
    Map-Based Genome Mining Method for Identifying Regulatory Loci Controlling the Level of Gene Transcripts and Products 失效

    公开(公告)号：US20080233574A1

    公开(公告)日：2008-09-25

    申请号：US11923946

    申请日：2007-10-25

    申请人： Yves Castonguay ,  Louise S. O'Donoughue ,  Serge Laberge ,  Antonio F. Monroy ,  Louis P. Vezina ,  Benoit S. Landry

    发明人： Yves Castonguay ,  Louise S. O'Donoughue ,  Serge Laberge ,  Antonio F. Monroy ,  Louis P. Vezina ,  Benoit S. Landry

    IPC分类号： C12Q1/68 ,  C12N15/87

    CPC分类号： C12Q1/6897 ,  C12Q1/6809

    摘要： The invention pertains to a method for identifying one or more regions within a genome of an organism of interest that mediate the expression of one or more genes of interest. The method comprises identifying a first and a second organism of interest, the first organism of interest is characterized by exhibiting a measurable response to an environmental stimulus, or otherwise, exhibiting a phenotype associated with differential gene expression associated with a process of interest. The second organism of interest is characterized by lacking or not exhibit as strong a response to the stimulus as that observed within the first organism of interest, or it exhibits a different phenotype compared with that of the first organism of interest, wherein the different phenotype is associated with the process of interest, or it exhibits a phenotype of interest that segregates when compared with the first organism of interest, or a combination thereof. The first and second organisms of interest are crossed to produce a population of segregated progeny and RNA is extracted from each of the segregated progeny. The level of gene expression for one or more genes of interest that are associated with the response to an environmental stimulus, or process of interest is quantified. A linkage map of the segregated progeny using one or more markers is prepared, and the relationship between said one or more markers on the linkage map and the gene expression of the one or more genes of interest is determined and one or more quantitative trait loci (QTL) identified. This method also pertains to identifying one or more QTLs associated with one or more genes of interest in segregated progeny that are subjected a desired environmental stimulus. Furthermore, this method may be used for the identification of one or more QTL corresponding to a transcription factor or any factor controlling the expression of one or more genes of interest, and the one or more genes located at the one or more QTL may be isolated and characterized.

    Map-based genome mining method for identifying regulatory loci controlling the level of gene transcripts and products
    3.
    发明授权
    Map-based genome mining method for identifying regulatory loci controlling the level of gene transcripts and products 失效
    基于地图的基因组挖掘方法，用于鉴定控制基因转录本和产物水平的调控基因座

    公开(公告)号：US08090541B2

    公开(公告)日：2012-01-03

    申请号：US11923946

    申请日：2007-10-25

    申请人： Yves Castonguay ,  Louise S. O'Donoughue ,  Serge Laberge ,  Antonio F. Monroy ,  Louis P. Vezina ,  Benoit S. Landry

    发明人： Yves Castonguay ,  Louise S. O'Donoughue ,  Serge Laberge ,  Antonio F. Monroy ,  Louis P. Vezina ,  Benoit S. Landry

    IPC分类号： G01N33/48

    CPC分类号： C12Q1/6897 ,  C12Q1/6809

    摘要： A method is provided for identifying one or more regions within a genome of an organism of interest that mediate the expression of one or more genes of interest. The method comprises identifying a first organism of interest exhibiting a measurable response to an environmental stimulus; identifying a second organism of interest that lacks or does not exhibit as strong a response to the stimulus as compared with the first organism of interest; crossing the first and second organisms of interest to produce a population of progeny; extracting RNA from each individual population of progeny; quantifying a level of gene expression for one or more genes of interest that are associated with the response to an environmental stimulus; identifying one or more Quantitative Trait Locus (QTL), wherein gene expression level is a quantitative trait, and using one or more markers comprising one or more regulatory sequences to mediate said expression of said one or more genes of interest induced by said environmental stimulus; and identifying the one or more regulatory sequences located at said one or more QTL.

    摘要翻译： 提供了一种用于鉴定介导一种或多种感兴趣的基因的表达的感兴趣生物体的基因组内的一个或多个区域的方法。 该方法包括鉴定对环境刺激具有可测量响应的感兴趣的第一生物体; 识别与所关注的第一有机体相比，缺乏或不表现出对刺激的强烈反应的目的的第二生物体; 穿过感兴趣的第一和第二生物产生一群后代; 从每个个体的后代提取RNA; 量化与对环境刺激的反应相关的一种或多种感兴趣的基因的基因表达水平; 鉴定一个或多个定量性状基因座（QTL），其中基因表达水平是数量性状，并且使用一种或多种包含一种或多种调节序列的标记来介导由所述环境刺激诱导的所述一种或多种感兴趣的基因的所述表达; 以及鉴定位于所述一个或多个QTL处的一个或多个调节序列。

    Protein production in transgenic alfalfa plants
    5.
    发明授权
    Protein production in transgenic alfalfa plants 失效

    公开(公告)号：US5990385A

    公开(公告)日：1999-11-23

    申请号：US968688

    申请日：1997-11-12

    申请人： Louis-P. Vezina ,  Serge Laberge ,  Renee Bazin ,  Habib Khoudi ,  Real Lemieux ,  Guy Allard

    发明人： Louis-P. Vezina ,  Serge Laberge ,  Renee Bazin ,  Habib Khoudi ,  Real Lemieux ,  Guy Allard

    IPC分类号： C07K14/705 ,  C12N15/13 ,  C12N15/82 ,  A01H1/02 ,  C07K1/14 ,  C07K1/22

    CPC分类号： C07K14/70503 ,  C12N15/8258

    摘要： This invention is directed to characterizing a host system suitable for the production of functional transgenic proteins, such as anti-human IgG, for use in applications requiring Government regulatory approval. It is well known that regulatory agencies required stable, consistent master cell banks and master cell lines for the production of transgenic proteins in order to ensure sufficient material for appropriate characterization, clinical trials, and potential sales. Current plant production systems require the establishment of seed banks for this purpose. However, there are many draw backs related to such a system for the production of a continuous reliable transgenic protein source. An aspect of this invention is directed to characterizing a plant production system suitable for transgenic proteins that meet the stringent regulatory requirements. Another aspect of this invention exemplifies the production and characterization of an anti-human IgG for use as a blood grouping reagents, through the expression of corresponding genes in transgenic alfalfa plants. The cDNAs of the heavy and light chains of a human IgG-specific IgG2a(kappa) murine mAb (C5-1) were transferred into alfalfa through Agrobacterium infection. Transgenic plants expressing the light- and heavy-chain encoding mRNAs were obtained and plants from the F1 progeny (obtained by sexual crossing) were found to express fully assembled C5-1. Furthermore, the transgenic protein was stable in vivo, as well as during extraction and purification procedures. Purification yielded a unique H2L2 form with a reactivity indistinguishable from hybridoma-derived C5-1 in standardized serological tests. Results indicate that plant-derived transgenic proteins, such as mAbs can be used as diagnostic reagents as effectively as hybridoma-derived mAbs, and demonstrates the usefulness of the transformed alfalfa system to produce large amounts of proteins, including multimeric proteins such as mAbs.